A combined use of silver pretreatment and impregnation with consequent Nissl staining for cortex and striatum architectonics study
Despite a rapid growth in the application of modern techniques for visualization studies in life sciences, the classical methods of histological examination are yet to be outdated. Herein, we introduce a new approach that involves combining silver nitrate pretreatment and impregnation with consequen...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Neuroanatomy |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnana.2022.940993/full |
| _version_ | 1798019923207782400 |
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| author | Gennadii Piavchenko Vladislav Soldatov Artem Venediktov Natalia Kartashkina Natalia Novikova Marina Gorbunova Tatiana Boronikhina Alexander Yatskovskiy Igor Meglinski Igor Meglinski Sergey Kuznetsov |
| author_facet | Gennadii Piavchenko Vladislav Soldatov Artem Venediktov Natalia Kartashkina Natalia Novikova Marina Gorbunova Tatiana Boronikhina Alexander Yatskovskiy Igor Meglinski Igor Meglinski Sergey Kuznetsov |
| author_sort | Gennadii Piavchenko |
| collection | DOAJ |
| description | Despite a rapid growth in the application of modern techniques for visualization studies in life sciences, the classical methods of histological examination are yet to be outdated. Herein, we introduce a new approach that involves combining silver nitrate pretreatment and impregnation with consequent Nissl (cresyl violet) staining for cortex and striatum architectonics study on the same microscopy slide. The developed approach of hybrid staining provides a high-quality visualization of cellular and subcellular structures, including impregnated neurons (about 10%), Nissl-stained neurons (all the remaining ones), and astrocytes, as well as chromatophilic substances, nucleoli, and neuropil in paraffin sections. We provide a comparative study of the neuronal architectonics in both the motor cortex and striatum based on the differences in their tinctorial properties. In addition to a comparative study of the neuronal architectonics in both the motor cortex and striatum, the traditional methods to stain the cortex (motor and piriform) and the striatum are considered. The proposed staining approach compiles the routine conventional methods for thin sections, expanding avenues for more advanced examination of neurons, blood–brain barrier components, and fibers both under normal and pathological conditions. One of the main hallmarks of our method is the ability to detect changes in the number of glial cells. The results of astrocyte visualization in the motor cortex obtained by the developed technique agree well with the alternative studies by glial fibrillary acidic protein (GFAP) immunohistochemical reaction. The presented approach of combined staining has great potential in current histological practice, in particular for the evaluation of several neurological disorders in clinical, pre-clinical, or neurobiological animal studies. |
| first_indexed | 2024-04-11T16:49:17Z |
| format | Article |
| id | doaj.art-c5fd6705144d4779ae9bd874f495d824 |
| institution | Directory Open Access Journal |
| issn | 1662-5129 |
| language | English |
| last_indexed | 2024-04-11T16:49:17Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroanatomy |
| spelling | doaj.art-c5fd6705144d4779ae9bd874f495d8242022-12-22T04:13:30ZengFrontiers Media S.A.Frontiers in Neuroanatomy1662-51292022-10-011610.3389/fnana.2022.940993940993A combined use of silver pretreatment and impregnation with consequent Nissl staining for cortex and striatum architectonics studyGennadii Piavchenko0Vladislav Soldatov1Artem Venediktov2Natalia Kartashkina3Natalia Novikova4Marina Gorbunova5Tatiana Boronikhina6Alexander Yatskovskiy7Igor Meglinski8Igor Meglinski9Sergey Kuznetsov10Department of Histology, Cytology, and Embryology, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, RussiaDepartment of Pharmacology and Clinical Pharmacology, Belgorod National Research University, Belgorod, RussiaDepartment of Histology, Cytology, and Embryology, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, RussiaDepartment of Histology, Cytology, and Embryology, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, RussiaLaboratory of Pathophysiology, Institute of Experimental Medicine, Saint Petersburg, RussiaDepartment of Histology, Cytology, and Embryology, Orel State University named after I.S. Turgenev, Orel, RussiaDepartment of Histology, Cytology, and Embryology, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, RussiaDepartment of Histology, Cytology, and Embryology, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, RussiaOpto-Electronics and Measurement Techniques, Faculty of Information and Electrical Engineering, University of Oulu, Oulu, FinlandCollege of Engineering and Applied Science, Aston University, Birmingham, United KingdomDepartment of Histology, Cytology, and Embryology, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, RussiaDespite a rapid growth in the application of modern techniques for visualization studies in life sciences, the classical methods of histological examination are yet to be outdated. Herein, we introduce a new approach that involves combining silver nitrate pretreatment and impregnation with consequent Nissl (cresyl violet) staining for cortex and striatum architectonics study on the same microscopy slide. The developed approach of hybrid staining provides a high-quality visualization of cellular and subcellular structures, including impregnated neurons (about 10%), Nissl-stained neurons (all the remaining ones), and astrocytes, as well as chromatophilic substances, nucleoli, and neuropil in paraffin sections. We provide a comparative study of the neuronal architectonics in both the motor cortex and striatum based on the differences in their tinctorial properties. In addition to a comparative study of the neuronal architectonics in both the motor cortex and striatum, the traditional methods to stain the cortex (motor and piriform) and the striatum are considered. The proposed staining approach compiles the routine conventional methods for thin sections, expanding avenues for more advanced examination of neurons, blood–brain barrier components, and fibers both under normal and pathological conditions. One of the main hallmarks of our method is the ability to detect changes in the number of glial cells. The results of astrocyte visualization in the motor cortex obtained by the developed technique agree well with the alternative studies by glial fibrillary acidic protein (GFAP) immunohistochemical reaction. The presented approach of combined staining has great potential in current histological practice, in particular for the evaluation of several neurological disorders in clinical, pre-clinical, or neurobiological animal studies.https://www.frontiersin.org/articles/10.3389/fnana.2022.940993/fullstaining combinationNissl stainingsilver impregnationprotoplasmic astrocytesartificial pseudostaining |
| spellingShingle | Gennadii Piavchenko Vladislav Soldatov Artem Venediktov Natalia Kartashkina Natalia Novikova Marina Gorbunova Tatiana Boronikhina Alexander Yatskovskiy Igor Meglinski Igor Meglinski Sergey Kuznetsov A combined use of silver pretreatment and impregnation with consequent Nissl staining for cortex and striatum architectonics study Frontiers in Neuroanatomy staining combination Nissl staining silver impregnation protoplasmic astrocytes artificial pseudostaining |
| title | A combined use of silver pretreatment and impregnation with consequent Nissl staining for cortex and striatum architectonics study |
| title_full | A combined use of silver pretreatment and impregnation with consequent Nissl staining for cortex and striatum architectonics study |
| title_fullStr | A combined use of silver pretreatment and impregnation with consequent Nissl staining for cortex and striatum architectonics study |
| title_full_unstemmed | A combined use of silver pretreatment and impregnation with consequent Nissl staining for cortex and striatum architectonics study |
| title_short | A combined use of silver pretreatment and impregnation with consequent Nissl staining for cortex and striatum architectonics study |
| title_sort | combined use of silver pretreatment and impregnation with consequent nissl staining for cortex and striatum architectonics study |
| topic | staining combination Nissl staining silver impregnation protoplasmic astrocytes artificial pseudostaining |
| url | https://www.frontiersin.org/articles/10.3389/fnana.2022.940993/full |
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