Identification of PDGFRα-positive interstitial cells in the distal segment of the murine vas deferens
Abstract Platelet-derived growth factor receptor-α (PDGFRα)-positive interstitial cells (ICs) are widely distributed in various organs and may be involved in the motility of various tubular organs. We, for the first time, aimed to investigate the distribution, immunohistochemical characteristics, an...
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| Sprache: | English |
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Nature Portfolio
2021-04-01
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| Schriftenreihe: | Scientific Reports |
| Online Zugang: | https://doi.org/10.1038/s41598-021-87049-6 |
| _version_ | 1831730034787745792 |
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| author | Tasuku Hiroshige Kei-Ichiro Uemura Shingo Hirashima Kiyosato Hino Akinobu Togo Keisuke Ohta Tsukasa Igawa Kei-Ichiro Nakamura |
| author_facet | Tasuku Hiroshige Kei-Ichiro Uemura Shingo Hirashima Kiyosato Hino Akinobu Togo Keisuke Ohta Tsukasa Igawa Kei-Ichiro Nakamura |
| author_sort | Tasuku Hiroshige |
| collection | DOAJ |
| description | Abstract Platelet-derived growth factor receptor-α (PDGFRα)-positive interstitial cells (ICs) are widely distributed in various organs and may be involved in the motility of various tubular organs. We, for the first time, aimed to investigate the distribution, immunohistochemical characteristics, and ultrastructure of PDGFRα-positive ICs in murine vas deferens, using confocal laser scanning microscopy, transmission electron microscopy (TEM), and immuno-electron microscopy (immuno-EM). For immunofluorescence, we used antibodies against PDGFRα and other markers of ICs. PDGFRα-positive ICs were distributed widely in the lamina propria, smooth muscles, and serosal layers. Although most PDGFRα-positive ICs labeled CD34, they did not label CD34 in the subepithelial layers. Additionally, PDGFRα-positive ICs were in close proximity to each other, as also to the surrounding cells. TEM and immuno-EM findings revealed that PDGFRα-positive ICs established close physical interactions with adjacent ICs. Extracellular vesicles were also detected around the PDGFRα-positive ICs. Our morphological findings suggest that PDGFRα-positive ICs may have several subpopulations, which can play an important role in intercellular signaling via direct contact with the IC network and the extracellular vesicles in the murine vas deferens. Further investigation on PDGFRα-positive ICs in the vas deferens may lead to understanding the vas deferens mortility. |
| first_indexed | 2024-12-21T09:38:03Z |
| format | Article |
| id | doaj.art-c60511263b4d4fe9a8f6f45f4fa5372a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-21T09:38:03Z |
| publishDate | 2021-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c60511263b4d4fe9a8f6f45f4fa5372a2022-12-21T19:08:34ZengNature PortfolioScientific Reports2045-23222021-04-0111111410.1038/s41598-021-87049-6Identification of PDGFRα-positive interstitial cells in the distal segment of the murine vas deferensTasuku Hiroshige0Kei-Ichiro Uemura1Shingo Hirashima2Kiyosato Hino3Akinobu Togo4Keisuke Ohta5Tsukasa Igawa6Kei-Ichiro Nakamura7Division of Microscopic and Development Anatomy, Department of Anatomy, Kurume University School of MedicineDepartment of Urology, Kurume University School of MedicineDivision of Microscopic and Development Anatomy, Department of Anatomy, Kurume University School of MedicineDivision of Microscopic and Development Anatomy, Department of Anatomy, Kurume University School of MedicineAdvanced Imaging Research Center, Kurume University School of MedicineDivision of Microscopic and Development Anatomy, Department of Anatomy, Kurume University School of MedicineDepartment of Urology, Kurume University School of MedicineDivision of Microscopic and Development Anatomy, Department of Anatomy, Kurume University School of MedicineAbstract Platelet-derived growth factor receptor-α (PDGFRα)-positive interstitial cells (ICs) are widely distributed in various organs and may be involved in the motility of various tubular organs. We, for the first time, aimed to investigate the distribution, immunohistochemical characteristics, and ultrastructure of PDGFRα-positive ICs in murine vas deferens, using confocal laser scanning microscopy, transmission electron microscopy (TEM), and immuno-electron microscopy (immuno-EM). For immunofluorescence, we used antibodies against PDGFRα and other markers of ICs. PDGFRα-positive ICs were distributed widely in the lamina propria, smooth muscles, and serosal layers. Although most PDGFRα-positive ICs labeled CD34, they did not label CD34 in the subepithelial layers. Additionally, PDGFRα-positive ICs were in close proximity to each other, as also to the surrounding cells. TEM and immuno-EM findings revealed that PDGFRα-positive ICs established close physical interactions with adjacent ICs. Extracellular vesicles were also detected around the PDGFRα-positive ICs. Our morphological findings suggest that PDGFRα-positive ICs may have several subpopulations, which can play an important role in intercellular signaling via direct contact with the IC network and the extracellular vesicles in the murine vas deferens. Further investigation on PDGFRα-positive ICs in the vas deferens may lead to understanding the vas deferens mortility.https://doi.org/10.1038/s41598-021-87049-6 |
| spellingShingle | Tasuku Hiroshige Kei-Ichiro Uemura Shingo Hirashima Kiyosato Hino Akinobu Togo Keisuke Ohta Tsukasa Igawa Kei-Ichiro Nakamura Identification of PDGFRα-positive interstitial cells in the distal segment of the murine vas deferens Scientific Reports |
| title | Identification of PDGFRα-positive interstitial cells in the distal segment of the murine vas deferens |
| title_full | Identification of PDGFRα-positive interstitial cells in the distal segment of the murine vas deferens |
| title_fullStr | Identification of PDGFRα-positive interstitial cells in the distal segment of the murine vas deferens |
| title_full_unstemmed | Identification of PDGFRα-positive interstitial cells in the distal segment of the murine vas deferens |
| title_short | Identification of PDGFRα-positive interstitial cells in the distal segment of the murine vas deferens |
| title_sort | identification of pdgfrα positive interstitial cells in the distal segment of the murine vas deferens |
| url | https://doi.org/10.1038/s41598-021-87049-6 |
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