Degenerative Changes in the Claustrum and Endopiriform Nucleus after Early-Life Status Epilepticus in Rats
The aim of the present study was to analyze the location of degenerating neurons in the dorsal (insular) claustrum (DCL, VCL) and the dorsal, intermediate and ventral endopiriform nucleus (DEn, IEn, VEn) in rat pups following lithium–pilocarpine status epilepticus (SE) induced at postnatal days [P]1...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-01-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/25/2/1296 |
| _version_ | 1797339771927789568 |
|---|---|
| author | Rastislav Druga Pavel Mares Martin Salaj Hana Kubova |
| author_facet | Rastislav Druga Pavel Mares Martin Salaj Hana Kubova |
| author_sort | Rastislav Druga |
| collection | DOAJ |
| description | The aim of the present study was to analyze the location of degenerating neurons in the dorsal (insular) claustrum (DCL, VCL) and the dorsal, intermediate and ventral endopiriform nucleus (DEn, IEn, VEn) in rat pups following lithium–pilocarpine status epilepticus (SE) induced at postnatal days [P]12, 15, 18, 21 and 25. The presence of Fluoro-Jade B-positive neurons was evaluated at 4, 12, 24, 48 h and 1 week later. A small number of degenerated neurons was observed in the CL, as well as in the DEn at P12 and P15. The number of degenerated neurons was increased in the CL as well as in the DEn at P18 and above and was highest at longer survival intervals. The CL at P15 and 18 contained a small or moderate number of degenerated neurons mainly close to the medial and dorsal margins also designated as DCl (“shell”) while isolated degenerated neurons were distributed in the VCl (“core”). In P21 and 25, a larger number of degenerated neurons occurred in both subdivisions of the dorsal claustrum. The majority of degenerated neurons in the endopiriform nucleus were found in the intermediate and caudal third of the DEn. A small number of degenerated neurons was dispersed in the whole extent of the DEn with prevalence to its medial margin. Our results indicate that degenerated neurons in the claustrum CL and endopiriform nucleus are distributed mainly in subdivisions originating from the ventral pallium; their distribution correlates with chemoarchitectonics of both nuclei and with their intrinsic and extrinsic connections. |
| first_indexed | 2024-03-08T09:53:13Z |
| format | Article |
| id | doaj.art-c60c4d2edbd746dab444b25996f4cafb |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T09:53:13Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c60c4d2edbd746dab444b25996f4cafb2024-01-29T13:59:22ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-01-01252129610.3390/ijms25021296Degenerative Changes in the Claustrum and Endopiriform Nucleus after Early-Life Status Epilepticus in RatsRastislav Druga0Pavel Mares1Martin Salaj2Hana Kubova3Institute of Anatomy, 2nd Medical Faculty, Charles University, 15006 Prague, Czech RepublicLaboratory of Developmental Epileptology, Institute of Physiology, Czech Academy of Sciences, 14200 Prague, Czech RepublicInstitute of Anatomy, 2nd Medical Faculty, Charles University, 15006 Prague, Czech RepublicLaboratory of Developmental Epileptology, Institute of Physiology, Czech Academy of Sciences, 14200 Prague, Czech RepublicThe aim of the present study was to analyze the location of degenerating neurons in the dorsal (insular) claustrum (DCL, VCL) and the dorsal, intermediate and ventral endopiriform nucleus (DEn, IEn, VEn) in rat pups following lithium–pilocarpine status epilepticus (SE) induced at postnatal days [P]12, 15, 18, 21 and 25. The presence of Fluoro-Jade B-positive neurons was evaluated at 4, 12, 24, 48 h and 1 week later. A small number of degenerated neurons was observed in the CL, as well as in the DEn at P12 and P15. The number of degenerated neurons was increased in the CL as well as in the DEn at P18 and above and was highest at longer survival intervals. The CL at P15 and 18 contained a small or moderate number of degenerated neurons mainly close to the medial and dorsal margins also designated as DCl (“shell”) while isolated degenerated neurons were distributed in the VCl (“core”). In P21 and 25, a larger number of degenerated neurons occurred in both subdivisions of the dorsal claustrum. The majority of degenerated neurons in the endopiriform nucleus were found in the intermediate and caudal third of the DEn. A small number of degenerated neurons was dispersed in the whole extent of the DEn with prevalence to its medial margin. Our results indicate that degenerated neurons in the claustrum CL and endopiriform nucleus are distributed mainly in subdivisions originating from the ventral pallium; their distribution correlates with chemoarchitectonics of both nuclei and with their intrinsic and extrinsic connections.https://www.mdpi.com/1422-0067/25/2/1296status epilepticusclaustrumendopiriform nucleusclaustroamygdaloid complexneurodegenerationontogeny |
| spellingShingle | Rastislav Druga Pavel Mares Martin Salaj Hana Kubova Degenerative Changes in the Claustrum and Endopiriform Nucleus after Early-Life Status Epilepticus in Rats International Journal of Molecular Sciences status epilepticus claustrum endopiriform nucleus claustroamygdaloid complex neurodegeneration ontogeny |
| title | Degenerative Changes in the Claustrum and Endopiriform Nucleus after Early-Life Status Epilepticus in Rats |
| title_full | Degenerative Changes in the Claustrum and Endopiriform Nucleus after Early-Life Status Epilepticus in Rats |
| title_fullStr | Degenerative Changes in the Claustrum and Endopiriform Nucleus after Early-Life Status Epilepticus in Rats |
| title_full_unstemmed | Degenerative Changes in the Claustrum and Endopiriform Nucleus after Early-Life Status Epilepticus in Rats |
| title_short | Degenerative Changes in the Claustrum and Endopiriform Nucleus after Early-Life Status Epilepticus in Rats |
| title_sort | degenerative changes in the claustrum and endopiriform nucleus after early life status epilepticus in rats |
| topic | status epilepticus claustrum endopiriform nucleus claustroamygdaloid complex neurodegeneration ontogeny |
| url | https://www.mdpi.com/1422-0067/25/2/1296 |
| work_keys_str_mv | AT rastislavdruga degenerativechangesintheclaustrumandendopiriformnucleusafterearlylifestatusepilepticusinrats AT pavelmares degenerativechangesintheclaustrumandendopiriformnucleusafterearlylifestatusepilepticusinrats AT martinsalaj degenerativechangesintheclaustrumandendopiriformnucleusafterearlylifestatusepilepticusinrats AT hanakubova degenerativechangesintheclaustrumandendopiriformnucleusafterearlylifestatusepilepticusinrats |