Analysis of phenolic compounds, antioxidant and antimicrobial properties of some endemic medicinal plants
This study was designed to evaluate the phenolic compounds and the in vitro antioxidant and antimicrobial activities of Onobrychis nitida, Hedysarum cappadocicum, Ebenus laguroides and Ebenus macrophylla which are medicinal plants and endemic for the flora of Turkey. The RP-HPLC-DAD (reverse phase-h...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
International Journal of Secondary Metabolite
2018-07-01
|
| Series: | International Journal of Secondary Metabolite |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/pub/ijsm/issue/34119/392354?publisher=ijate |
| _version_ | 1797906051638493184 |
|---|---|
| author | Ersan Bektaş Kaan Kaltalıoğlu Hüseyin Şahin Zafer Türkmen Ali Kandemir |
| author_facet | Ersan Bektaş Kaan Kaltalıoğlu Hüseyin Şahin Zafer Türkmen Ali Kandemir |
| author_sort | Ersan Bektaş |
| collection | DOAJ |
| description | This
study was designed to evaluate the phenolic compounds and the in vitro antioxidant and antimicrobial
activities of Onobrychis nitida,
Hedysarum cappadocicum, Ebenus laguroides and Ebenus macrophylla which are
medicinal plants and endemic for the flora of Turkey. The RP-HPLC-DAD (reverse
phase-high performance liquid chromatography with a diode array detector) was
used to evaluate the phenolic contents. The antioxidant properties were
determined to use total phenolic content (TPC), ferric reducing antioxidant
power (FRAP), and DPPH• radical scavenging activity assays. Antibacterial tests
were performed against 11 different microorganisms by using the microwell
dilution method. Each of the plant extracts were confirmed by bioactive assays
which demonstrated a significant activity due to different chemical
characteristics. Especially, rutin was the dominant component in Ebenus species with 19.434-11.808 mg
phenolic/g extract. While the highest phenolic content (101.73 mg gallic acid
equivalent/g extract) was observed in O.
nitida, the highest FRAP value was in E.
laguroides (719.09 μM FeSO4.7H2O equivalent), and the
strongest DPPH degree was in the E.
macrophylla extract with IC50: 69.45 μg/mL, respectively. Although plant extracts didn’t have efficient
values for antimicrobial activity, the slight effect was arisen in O. nitida against B. subtilis and S. aureus.
The results showed that all the extracts could be used in pharmacological or
dietary applications due to their valuable properties. |
| first_indexed | 2024-04-10T10:14:55Z |
| format | Article |
| id | doaj.art-c61245a16fb94a728394df275ccd9c3f |
| institution | Directory Open Access Journal |
| issn | 2148-6905 |
| language | English |
| last_indexed | 2024-04-10T10:14:55Z |
| publishDate | 2018-07-01 |
| publisher | International Journal of Secondary Metabolite |
| record_format | Article |
| series | International Journal of Secondary Metabolite |
| spelling | doaj.art-c61245a16fb94a728394df275ccd9c3f2023-02-15T16:21:58ZengInternational Journal of Secondary MetaboliteInternational Journal of Secondary Metabolite2148-69052018-07-0152758610.21448/ijsm.392354618Analysis of phenolic compounds, antioxidant and antimicrobial properties of some endemic medicinal plantsErsan BektaşKaan KaltalıoğluHüseyin ŞahinZafer TürkmenAli KandemirThis study was designed to evaluate the phenolic compounds and the in vitro antioxidant and antimicrobial activities of Onobrychis nitida, Hedysarum cappadocicum, Ebenus laguroides and Ebenus macrophylla which are medicinal plants and endemic for the flora of Turkey. The RP-HPLC-DAD (reverse phase-high performance liquid chromatography with a diode array detector) was used to evaluate the phenolic contents. The antioxidant properties were determined to use total phenolic content (TPC), ferric reducing antioxidant power (FRAP), and DPPH• radical scavenging activity assays. Antibacterial tests were performed against 11 different microorganisms by using the microwell dilution method. Each of the plant extracts were confirmed by bioactive assays which demonstrated a significant activity due to different chemical characteristics. Especially, rutin was the dominant component in Ebenus species with 19.434-11.808 mg phenolic/g extract. While the highest phenolic content (101.73 mg gallic acid equivalent/g extract) was observed in O. nitida, the highest FRAP value was in E. laguroides (719.09 μM FeSO4.7H2O equivalent), and the strongest DPPH degree was in the E. macrophylla extract with IC50: 69.45 μg/mL, respectively. Although plant extracts didn’t have efficient values for antimicrobial activity, the slight effect was arisen in O. nitida against B. subtilis and S. aureus. The results showed that all the extracts could be used in pharmacological or dietary applications due to their valuable properties.https://dergipark.org.tr/tr/pub/ijsm/issue/34119/392354?publisher=ijateonobrychis nitidahedysarum cappadocicumebenus laguroidesebenus macrophyllaphenolicsbioactivity |
| spellingShingle | Ersan Bektaş Kaan Kaltalıoğlu Hüseyin Şahin Zafer Türkmen Ali Kandemir Analysis of phenolic compounds, antioxidant and antimicrobial properties of some endemic medicinal plants International Journal of Secondary Metabolite onobrychis nitida hedysarum cappadocicum ebenus laguroides ebenus macrophylla phenolics bioactivity |
| title | Analysis of phenolic compounds, antioxidant and antimicrobial properties of some endemic medicinal plants |
| title_full | Analysis of phenolic compounds, antioxidant and antimicrobial properties of some endemic medicinal plants |
| title_fullStr | Analysis of phenolic compounds, antioxidant and antimicrobial properties of some endemic medicinal plants |
| title_full_unstemmed | Analysis of phenolic compounds, antioxidant and antimicrobial properties of some endemic medicinal plants |
| title_short | Analysis of phenolic compounds, antioxidant and antimicrobial properties of some endemic medicinal plants |
| title_sort | analysis of phenolic compounds antioxidant and antimicrobial properties of some endemic medicinal plants |
| topic | onobrychis nitida hedysarum cappadocicum ebenus laguroides ebenus macrophylla phenolics bioactivity |
| url | https://dergipark.org.tr/tr/pub/ijsm/issue/34119/392354?publisher=ijate |
| work_keys_str_mv | AT ersanbektas analysisofphenoliccompoundsantioxidantandantimicrobialpropertiesofsomeendemicmedicinalplants AT kaankaltalıoglu analysisofphenoliccompoundsantioxidantandantimicrobialpropertiesofsomeendemicmedicinalplants AT huseyinsahin analysisofphenoliccompoundsantioxidantandantimicrobialpropertiesofsomeendemicmedicinalplants AT zaferturkmen analysisofphenoliccompoundsantioxidantandantimicrobialpropertiesofsomeendemicmedicinalplants AT alikandemir analysisofphenoliccompoundsantioxidantandantimicrobialpropertiesofsomeendemicmedicinalplants |