Quantifying the global binding and target-search dynamics of epigenetic regulatory factors using live-cell single-molecule tracking

Quantifying the global binding and target-search dynamics of epigenetic regulatory factors using live-cell single-molecule tracking

Summary: This protocol provides instructions to track the global dynamics of single epigenetic regulatory factors in live cells. We describe an approach to generate cell lines that stably express HaloTag-fused proteins. We then use live-cell single-molecule tracking to obtain kinetic populations and...

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Bibliographic Details
Main Authors: Kyle Brown, Samantha Kent, Xiaojun Ren
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Biophysics
Single-molecule Assays
Cell Biology
Cell-based Assays
Microscopy
Gene Expression
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721006651

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