Characterization of the Brassica napus Flavonol Synthase Gene Family Reveals Bifunctional Flavonol Synthases
Flavonol synthase (FLS) is a key enzyme for the formation of flavonols, which are a subclass of the flavonoids. FLS catalyzes the conversion of dihydroflavonols to flavonols. The enzyme belongs to the 2-oxoglutarate-dependent dioxygenases (2-ODD) superfamily. We characterized the FLS gene family of...
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.733762/full |
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| author | Hanna Marie Schilbert Maximilian Schöne Thomas Baier Mareike Busche Prisca Viehöver Bernd Weisshaar Daniela Holtgräwe |
| author_facet | Hanna Marie Schilbert Maximilian Schöne Thomas Baier Mareike Busche Prisca Viehöver Bernd Weisshaar Daniela Holtgräwe |
| author_sort | Hanna Marie Schilbert |
| collection | DOAJ |
| description | Flavonol synthase (FLS) is a key enzyme for the formation of flavonols, which are a subclass of the flavonoids. FLS catalyzes the conversion of dihydroflavonols to flavonols. The enzyme belongs to the 2-oxoglutarate-dependent dioxygenases (2-ODD) superfamily. We characterized the FLS gene family of Brassica napus that covers 13 genes, based on the genome sequence of the B. napus cultivar Express 617. The goal was to unravel which BnaFLS genes are relevant for seed flavonol accumulation in the amphidiploid species B. napus. Two BnaFLS1 homeologs were identified and shown to encode bifunctional enzymes. Both exhibit FLS activity as well as flavanone 3-hydroxylase (F3H) activity, which was demonstrated in vivo and in planta. BnaFLS1-1 and -2 are capable of converting flavanones into dihydroflavonols and further into flavonols. Analysis of spatio-temporal transcription patterns revealed similar expression profiles of BnaFLS1 genes. Both are mainly expressed in reproductive organs and co-expressed with the genes encoding early steps of flavonoid biosynthesis. Our results provide novel insights into flavonol biosynthesis in B. napus and contribute information for breeding targets with the aim to modify the flavonol content in rapeseed. |
| first_indexed | 2024-12-17T22:43:28Z |
| format | Article |
| id | doaj.art-c615703e05ba446ba4ef0fd0e61d0802 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-17T22:43:28Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-c615703e05ba446ba4ef0fd0e61d08022022-12-21T21:29:52ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-10-011210.3389/fpls.2021.733762733762Characterization of the Brassica napus Flavonol Synthase Gene Family Reveals Bifunctional Flavonol SynthasesHanna Marie Schilbert0Maximilian Schöne1Thomas Baier2Mareike Busche3Prisca Viehöver4Bernd Weisshaar5Daniela Holtgräwe6Genetics and Genomics of Plants, CeBiTec and Faculty of Biology, Bielefeld University, Bielefeld, GermanyGenetics and Genomics of Plants, CeBiTec and Faculty of Biology, Bielefeld University, Bielefeld, GermanyAlgae Biotechnology and Bioenergy, CeBiTec and Faculty of Biology, Bielefeld University, Bielefeld, GermanyGenetics and Genomics of Plants, CeBiTec and Faculty of Biology, Bielefeld University, Bielefeld, GermanyGenetics and Genomics of Plants, CeBiTec and Faculty of Biology, Bielefeld University, Bielefeld, GermanyGenetics and Genomics of Plants, CeBiTec and Faculty of Biology, Bielefeld University, Bielefeld, GermanyGenetics and Genomics of Plants, CeBiTec and Faculty of Biology, Bielefeld University, Bielefeld, GermanyFlavonol synthase (FLS) is a key enzyme for the formation of flavonols, which are a subclass of the flavonoids. FLS catalyzes the conversion of dihydroflavonols to flavonols. The enzyme belongs to the 2-oxoglutarate-dependent dioxygenases (2-ODD) superfamily. We characterized the FLS gene family of Brassica napus that covers 13 genes, based on the genome sequence of the B. napus cultivar Express 617. The goal was to unravel which BnaFLS genes are relevant for seed flavonol accumulation in the amphidiploid species B. napus. Two BnaFLS1 homeologs were identified and shown to encode bifunctional enzymes. Both exhibit FLS activity as well as flavanone 3-hydroxylase (F3H) activity, which was demonstrated in vivo and in planta. BnaFLS1-1 and -2 are capable of converting flavanones into dihydroflavonols and further into flavonols. Analysis of spatio-temporal transcription patterns revealed similar expression profiles of BnaFLS1 genes. Both are mainly expressed in reproductive organs and co-expressed with the genes encoding early steps of flavonoid biosynthesis. Our results provide novel insights into flavonol biosynthesis in B. napus and contribute information for breeding targets with the aim to modify the flavonol content in rapeseed.https://www.frontiersin.org/articles/10.3389/fpls.2021.733762/fullflavonoid biosynthesisspecialized metabolismrapeseed2-oxoglutarate-dependent dioxygenasesflavanone 3-hydroxylasebifunctionality |
| spellingShingle | Hanna Marie Schilbert Maximilian Schöne Thomas Baier Mareike Busche Prisca Viehöver Bernd Weisshaar Daniela Holtgräwe Characterization of the Brassica napus Flavonol Synthase Gene Family Reveals Bifunctional Flavonol Synthases Frontiers in Plant Science flavonoid biosynthesis specialized metabolism rapeseed 2-oxoglutarate-dependent dioxygenases flavanone 3-hydroxylase bifunctionality |
| title | Characterization of the Brassica napus Flavonol Synthase Gene Family Reveals Bifunctional Flavonol Synthases |
| title_full | Characterization of the Brassica napus Flavonol Synthase Gene Family Reveals Bifunctional Flavonol Synthases |
| title_fullStr | Characterization of the Brassica napus Flavonol Synthase Gene Family Reveals Bifunctional Flavonol Synthases |
| title_full_unstemmed | Characterization of the Brassica napus Flavonol Synthase Gene Family Reveals Bifunctional Flavonol Synthases |
| title_short | Characterization of the Brassica napus Flavonol Synthase Gene Family Reveals Bifunctional Flavonol Synthases |
| title_sort | characterization of the brassica napus flavonol synthase gene family reveals bifunctional flavonol synthases |
| topic | flavonoid biosynthesis specialized metabolism rapeseed 2-oxoglutarate-dependent dioxygenases flavanone 3-hydroxylase bifunctionality |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.733762/full |
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