Coenzyme Q2 is a universal substrate for the measurement of respiratory chain enzyme activities in trypanosomatids
The measurement of respiratory chain enzyme activities is an integral part of basic research as well as for specialized examinations in clinical biochemistry. Most of the enzymes use ubiquinone as one of their substrates. For current in vitro measurements, several hydrophilic analogues of native ubi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2019-01-01
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| Series: | Parasite |
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| Online Access: | https://www.parasite-journal.org/articles/parasite/full_html/2019/01/parasite180165/parasite180165.html |
| _version_ | 1827614698754801664 |
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| author | Čermáková Petra Kovalinka Tomáš Ferenczyová Kristína Horváth Anton |
| author_facet | Čermáková Petra Kovalinka Tomáš Ferenczyová Kristína Horváth Anton |
| author_sort | Čermáková Petra |
| collection | DOAJ |
| description | The measurement of respiratory chain enzyme activities is an integral part of basic research as well as for specialized examinations in clinical biochemistry. Most of the enzymes use ubiquinone as one of their substrates. For current in vitro measurements, several hydrophilic analogues of native ubiquinone are used depending on the enzyme and the workplace. We tested five readily available commercial analogues and we showed that Coenzyme Q2 is the most suitable for the measurement of all tested enzyme activities. Use of a single substrate in all laboratories for several respiratory chain enzymes will improve our ability to compare data, in addition to simplifying the stock of chemicals required for this type of research. |
| first_indexed | 2024-03-09T08:59:35Z |
| format | Article |
| id | doaj.art-c6164f2b7116426388b27559a588e5d6 |
| institution | Directory Open Access Journal |
| issn | 1776-1042 |
| language | English |
| last_indexed | 2024-03-09T08:59:35Z |
| publishDate | 2019-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | Parasite |
| spelling | doaj.art-c6164f2b7116426388b27559a588e5d62023-12-02T12:04:04ZengEDP SciencesParasite1776-10422019-01-01261710.1051/parasite/2019017parasite180165Coenzyme Q2 is a universal substrate for the measurement of respiratory chain enzyme activities in trypanosomatidsČermáková PetraKovalinka TomášFerenczyová KristínaHorváth AntonThe measurement of respiratory chain enzyme activities is an integral part of basic research as well as for specialized examinations in clinical biochemistry. Most of the enzymes use ubiquinone as one of their substrates. For current in vitro measurements, several hydrophilic analogues of native ubiquinone are used depending on the enzyme and the workplace. We tested five readily available commercial analogues and we showed that Coenzyme Q2 is the most suitable for the measurement of all tested enzyme activities. Use of a single substrate in all laboratories for several respiratory chain enzymes will improve our ability to compare data, in addition to simplifying the stock of chemicals required for this type of research.https://www.parasite-journal.org/articles/parasite/full_html/2019/01/parasite180165/parasite180165.htmlubiquinonerespiratory chainCoenzyme Q |
| spellingShingle | Čermáková Petra Kovalinka Tomáš Ferenczyová Kristína Horváth Anton Coenzyme Q2 is a universal substrate for the measurement of respiratory chain enzyme activities in trypanosomatids Parasite ubiquinone respiratory chain Coenzyme Q |
| title | Coenzyme Q2 is a universal substrate for the measurement of respiratory chain enzyme activities in trypanosomatids |
| title_full | Coenzyme Q2 is a universal substrate for the measurement of respiratory chain enzyme activities in trypanosomatids |
| title_fullStr | Coenzyme Q2 is a universal substrate for the measurement of respiratory chain enzyme activities in trypanosomatids |
| title_full_unstemmed | Coenzyme Q2 is a universal substrate for the measurement of respiratory chain enzyme activities in trypanosomatids |
| title_short | Coenzyme Q2 is a universal substrate for the measurement of respiratory chain enzyme activities in trypanosomatids |
| title_sort | coenzyme q2 is a universal substrate for the measurement of respiratory chain enzyme activities in trypanosomatids |
| topic | ubiquinone respiratory chain Coenzyme Q |
| url | https://www.parasite-journal.org/articles/parasite/full_html/2019/01/parasite180165/parasite180165.html |
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