Comparative study among lactophenol blue, lactophenol solution and proteinase-K lytic solution for rostellar hooks morphometry of Echinococcus granulosus protoscolices
Echinococcus granulosus is a tapeworm whose life cycle includes dogs and other canines as final hosts, while domestic and wild ungulates act as intermediate hosts for the tissue-invading larval stage (metacestode). E. granulosus has a worldwide geographical distribution. Protoscolices and rostellar...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2020-01-01
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| Series: | Helminthologia |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/helm-2020-0010 |
| _version_ | 1797758727135166464 |
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| author | Hammad S. J. Cavallero S. Al-Nasiri F. S. Amelio S. D᾿ |
| author_facet | Hammad S. J. Cavallero S. Al-Nasiri F. S. Amelio S. D᾿ |
| author_sort | Hammad S. J. |
| collection | DOAJ |
| description | Echinococcus granulosus is a tapeworm whose life cycle includes dogs and other canines as final hosts, while domestic and wild ungulates act as intermediate hosts for the tissue-invading larval stage (metacestode). E. granulosus has a worldwide geographical distribution. Protoscolices and rostellar hooks of E. granulosus are useful for diagnosis and rostellar hook morphometric features may be useful to discriminate E. granulosus and related species. The present study was aimed to determine a more suitable lytic solution and to obtain a clearest vision for performing morphometric studies on the rostellar hooks of E. granulosus protoscolices. Five fertile hydatid cyst samples were collected from sheep in Kirkuk slaughterhouse, Iraq, during June of 2015. According to the results of the present study, proteinase-K lytic solution is the best approach in morphometric analysis to get a clear vision of rostellar hooks and a safer usage in comparison with solutions containing lactophenol (lactophenol, lactophenol blue). |
| first_indexed | 2024-03-12T18:34:17Z |
| format | Article |
| id | doaj.art-c616acb8da314fb680d378066f1bd320 |
| institution | Directory Open Access Journal |
| issn | 1336-9083 |
| language | English |
| last_indexed | 2024-03-12T18:34:17Z |
| publishDate | 2020-01-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Helminthologia |
| spelling | doaj.art-c616acb8da314fb680d378066f1bd3202023-08-02T08:09:26ZengSciendoHelminthologia1336-90832020-01-01571637010.2478/helm-2020-0010helm-2020-0010Comparative study among lactophenol blue, lactophenol solution and proteinase-K lytic solution for rostellar hooks morphometry of Echinococcus granulosus protoscolicesHammad S. J.0Cavallero S.1Al-Nasiri F. S.2Amelio S. D᾿3Department of Biology, College of Science, Tikrit University, Tikrit, IraqDepartment of Public Health and Infectious Diseases, Sapienza University of Rome, P.le Aldo Moro 5, 00185Rome, ItalyDepartment of Biology, College of Science, Tikrit University, Tikrit, IraqDepartment of Public Health and Infectious Diseases, Sapienza University of Rome, P.le Aldo Moro 5, 00185Rome, ItalyEchinococcus granulosus is a tapeworm whose life cycle includes dogs and other canines as final hosts, while domestic and wild ungulates act as intermediate hosts for the tissue-invading larval stage (metacestode). E. granulosus has a worldwide geographical distribution. Protoscolices and rostellar hooks of E. granulosus are useful for diagnosis and rostellar hook morphometric features may be useful to discriminate E. granulosus and related species. The present study was aimed to determine a more suitable lytic solution and to obtain a clearest vision for performing morphometric studies on the rostellar hooks of E. granulosus protoscolices. Five fertile hydatid cyst samples were collected from sheep in Kirkuk slaughterhouse, Iraq, during June of 2015. According to the results of the present study, proteinase-K lytic solution is the best approach in morphometric analysis to get a clear vision of rostellar hooks and a safer usage in comparison with solutions containing lactophenol (lactophenol, lactophenol blue).https://doi.org/10.2478/helm-2020-0010echinococcus granulosusprotoscolicesrostellar hookmorphometric study |
| spellingShingle | Hammad S. J. Cavallero S. Al-Nasiri F. S. Amelio S. D᾿ Comparative study among lactophenol blue, lactophenol solution and proteinase-K lytic solution for rostellar hooks morphometry of Echinococcus granulosus protoscolices Helminthologia echinococcus granulosus protoscolices rostellar hook morphometric study |
| title | Comparative study among lactophenol blue, lactophenol solution and proteinase-K lytic solution for rostellar hooks morphometry of Echinococcus granulosus protoscolices |
| title_full | Comparative study among lactophenol blue, lactophenol solution and proteinase-K lytic solution for rostellar hooks morphometry of Echinococcus granulosus protoscolices |
| title_fullStr | Comparative study among lactophenol blue, lactophenol solution and proteinase-K lytic solution for rostellar hooks morphometry of Echinococcus granulosus protoscolices |
| title_full_unstemmed | Comparative study among lactophenol blue, lactophenol solution and proteinase-K lytic solution for rostellar hooks morphometry of Echinococcus granulosus protoscolices |
| title_short | Comparative study among lactophenol blue, lactophenol solution and proteinase-K lytic solution for rostellar hooks morphometry of Echinococcus granulosus protoscolices |
| title_sort | comparative study among lactophenol blue lactophenol solution and proteinase k lytic solution for rostellar hooks morphometry of echinococcus granulosus protoscolices |
| topic | echinococcus granulosus protoscolices rostellar hook morphometric study |
| url | https://doi.org/10.2478/helm-2020-0010 |
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