Microorganisms from corn stigma with biocontrol potential of Fusarium verticillioides
Abstract The mycotoxigenic fungus Fusarium verticillioides is the primary maize pathogen and causes the maize stalk and ear rot diseases with significant economic losses. Furthermore, the excessive use of fungicides to control F. verticillioides constitutes threats to the environment and human healt...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Instituto Internacional de Ecologia
2022-08-01
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| Series: | Brazilian Journal of Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100722&tlng=en |
| _version_ | 1817995452859023360 |
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| author | G. F. D. Diniz J. E. F. Figueiredo U. G. P. Lana M. S. Marins D. D. Silva L. V. Cota I. E. Marriel C. A. Oliveira-Paiva |
| author_facet | G. F. D. Diniz J. E. F. Figueiredo U. G. P. Lana M. S. Marins D. D. Silva L. V. Cota I. E. Marriel C. A. Oliveira-Paiva |
| author_sort | G. F. D. Diniz |
| collection | DOAJ |
| description | Abstract The mycotoxigenic fungus Fusarium verticillioides is the primary maize pathogen and causes the maize stalk and ear rot diseases with significant economic losses. Furthermore, the excessive use of fungicides to control F. verticillioides constitutes threats to the environment and human health. Thus, sustainable alternatives such as biological control are needed to minimize the hazards associated with the current method. Although much is known about the vulnerability of the maize silks as a gateway for several fungal pathogens invading the developing grains, studies on the chemical properties of silk extracts and their resident microbiota are scarce. This study isolated and characterized bacteria and fungi that colonize the maize stigma to assess new potential biocontrol agents. The samples were collected from maize fields in the Brazilian localities of Sete Lagoas-MG, Sidrolândia-MS, Sertaneja-PR, and Goiânia-GO. One hundred sixty-seven microorganisms were isolated, 46% endophytic and 54% epiphytic. First, the antagonist activity was evaluated by the agar disc diffusion method performed in triplicate, and 83% of the isolates showed antagonist activity against F. verticillioides. Then, the 42 most efficient isolates were identified based on the partial sequencing of the bacterial 16S rRNA gene and fungi ITS region. The bacteria belong to the genera Bacillus (57.1%), Burkholderia (23.8%), Achromobacter (7.1%), Pseudomonas (2.4%), and Serratia (2.4%), while the fungi are Penicillium (2.4%), Candida (2.4), and Aspergillus (2.4%). The results showed that microorganisms from maize stigma might represent new promising agents for F. verticillioides control. |
| first_indexed | 2024-04-14T02:07:22Z |
| format | Article |
| id | doaj.art-c61a461581434a55ba61d2b8a1f11de0 |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-04-14T02:07:22Z |
| publishDate | 2022-08-01 |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-c61a461581434a55ba61d2b8a1f11de02022-12-22T02:18:39ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43752022-08-018210.1590/1519-6984.262567Microorganisms from corn stigma with biocontrol potential of Fusarium verticillioidesG. F. D. Dinizhttps://orcid.org/0000-0001-7018-6574J. E. F. Figueiredohttps://orcid.org/0000-0002-9542-2315U. G. P. Lanahttps://orcid.org/0000-0003-3964-8966M. S. Marinshttps://orcid.org/0000-0002-3508-8204D. D. Silvahttps://orcid.org/0000-0002-4037-3739L. V. Cotahttps://orcid.org/0000-0003-4216-7610I. E. Marrielhttps://orcid.org/0000-0002-1670-0285C. A. Oliveira-Paivahttps://orcid.org/0000-0002-1874-2489Abstract The mycotoxigenic fungus Fusarium verticillioides is the primary maize pathogen and causes the maize stalk and ear rot diseases with significant economic losses. Furthermore, the excessive use of fungicides to control F. verticillioides constitutes threats to the environment and human health. Thus, sustainable alternatives such as biological control are needed to minimize the hazards associated with the current method. Although much is known about the vulnerability of the maize silks as a gateway for several fungal pathogens invading the developing grains, studies on the chemical properties of silk extracts and their resident microbiota are scarce. This study isolated and characterized bacteria and fungi that colonize the maize stigma to assess new potential biocontrol agents. The samples were collected from maize fields in the Brazilian localities of Sete Lagoas-MG, Sidrolândia-MS, Sertaneja-PR, and Goiânia-GO. One hundred sixty-seven microorganisms were isolated, 46% endophytic and 54% epiphytic. First, the antagonist activity was evaluated by the agar disc diffusion method performed in triplicate, and 83% of the isolates showed antagonist activity against F. verticillioides. Then, the 42 most efficient isolates were identified based on the partial sequencing of the bacterial 16S rRNA gene and fungi ITS region. The bacteria belong to the genera Bacillus (57.1%), Burkholderia (23.8%), Achromobacter (7.1%), Pseudomonas (2.4%), and Serratia (2.4%), while the fungi are Penicillium (2.4%), Candida (2.4), and Aspergillus (2.4%). The results showed that microorganisms from maize stigma might represent new promising agents for F. verticillioides control.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100722&tlng=enmicroorganismsantagonistsFusarium moniliformebiofungicidephytopathogenmaize |
| spellingShingle | G. F. D. Diniz J. E. F. Figueiredo U. G. P. Lana M. S. Marins D. D. Silva L. V. Cota I. E. Marriel C. A. Oliveira-Paiva Microorganisms from corn stigma with biocontrol potential of Fusarium verticillioides Brazilian Journal of Biology microorganisms antagonists Fusarium moniliforme biofungicide phytopathogen maize |
| title | Microorganisms from corn stigma with biocontrol potential of Fusarium verticillioides |
| title_full | Microorganisms from corn stigma with biocontrol potential of Fusarium verticillioides |
| title_fullStr | Microorganisms from corn stigma with biocontrol potential of Fusarium verticillioides |
| title_full_unstemmed | Microorganisms from corn stigma with biocontrol potential of Fusarium verticillioides |
| title_short | Microorganisms from corn stigma with biocontrol potential of Fusarium verticillioides |
| title_sort | microorganisms from corn stigma with biocontrol potential of fusarium verticillioides |
| topic | microorganisms antagonists Fusarium moniliforme biofungicide phytopathogen maize |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100722&tlng=en |
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