Surveillance and molecular epidemiology of Neisseria gonorrhoeae isolates in Shenzhen, China, 2010–2017
Objectives: The development and emergence of antimicrobial resistance in Neisseria gonorrhoeae (NG) have become a major public-health problem worldwide. This study aimed to analyse the antimicrobial susceptibility and molecular characteristics of NG isolates in Shenzhen, China. Methods: A total of 1...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716520302198 |
| _version_ | 1818612374831104000 |
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| author | Feng Wang Jing-Wei Liu Yi-Zhun Li Li-Jun Zhang Jing Huang Xiang-Sheng Chen Shao-Chun Chen Yue-Ping Yin |
| author_facet | Feng Wang Jing-Wei Liu Yi-Zhun Li Li-Jun Zhang Jing Huang Xiang-Sheng Chen Shao-Chun Chen Yue-Ping Yin |
| author_sort | Feng Wang |
| collection | DOAJ |
| description | Objectives: The development and emergence of antimicrobial resistance in Neisseria gonorrhoeae (NG) have become a major public-health problem worldwide. This study aimed to analyse the antimicrobial susceptibility and molecular characteristics of NG isolates in Shenzhen, China. Methods: A total of 1282 NG isolates were consecutively collected between 2010 and 2017. Patient demographic information was also collected. MICs of ceftriaxone, spectinomycin, ciprofloxacin, azithromycin and penicillin were determined by agar dilution. Isolates were genotyped using N. gonorrhoeae multi-antigen sequence typing (NG-MAST). Results: Among the isolates, 97.4% were resistant to ciprofloxacin and 68.2% to penicillin. Moreover, 5.0% showed decreased susceptibility to ceftriaxone (CROD) and 17.3% were resistant to azithromycin (AZM-R); 1.3% were simultaneously CROD and AZM-R. All isolates were susceptible to spectinomycin. Increasing ceftriaxone MICs were found from 2010 to 2017. A total of 427 sequence types (STs) and 68 genogroups were identified from 724 isolates. ST5061, ST3741 and ST1766 were observed across the study years. ST14638 (n = 3) was predominant among 32 CROD isolates. Prevalent STs were ST5061 (n = 6), ST1866 (n = 5) and ST11133 (n = 5) among 96 AZM-R isolates. Conclusions: A high prevalence of isolates resistant to ciprofloxacin and penicillin was found in this study. Azithromycin, one antimicrobial of dual antimicrobial therapy recommended by the WHO, showed a high prevalence of resistance. The other, ceftriaxone, can be used continuously in this region owing to lower resistance levels. However, the emergence of CROD and decreasing susceptibility to ceftriaxone indicate that continuous antimicrobial resistance surveillance is essential. |
| first_indexed | 2024-12-16T15:45:13Z |
| format | Article |
| id | doaj.art-c61c09bb726043279226dd09a6528684 |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-16T15:45:13Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-c61c09bb726043279226dd09a65286842022-12-21T22:25:50ZengElsevierJournal of Global Antimicrobial Resistance2213-71652020-12-0123269274Surveillance and molecular epidemiology of Neisseria gonorrhoeae isolates in Shenzhen, China, 2010–2017Feng Wang0Jing-Wei Liu1Yi-Zhun Li2Li-Jun Zhang3Jing Huang4Xiang-Sheng Chen5Shao-Chun Chen6Yue-Ping Yin7Shenzhen Center for Chronic Disease Control, Shenzhen, ChinaNational Center for STD Control, Chinese Center for Disease Control and Prevention, Nanjing, China; Institute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing, ChinaShenzhen Center for Chronic Disease Control, Shenzhen, ChinaShenzhen Maternity & Healthcare Hospital, Shenzhen, ChinaShenzhen Center for Chronic Disease Control, Shenzhen, ChinaNational Center for STD Control, Chinese Center for Disease Control and Prevention, Nanjing, China; Institute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing, ChinaNational Center for STD Control, Chinese Center for Disease Control and Prevention, Nanjing, China; Institute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing, China; Corresponding authors. Present address: Department of Reference Lab, Institute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 12 Jiangwangmiao Street, Nanjing 210042, China.National Center for STD Control, Chinese Center for Disease Control and Prevention, Nanjing, China; Institute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing, China; Corresponding authors. Present address: Department of Reference Lab, Institute of Dermatology and Hospital for Skin Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 12 Jiangwangmiao Street, Nanjing 210042, China.Objectives: The development and emergence of antimicrobial resistance in Neisseria gonorrhoeae (NG) have become a major public-health problem worldwide. This study aimed to analyse the antimicrobial susceptibility and molecular characteristics of NG isolates in Shenzhen, China. Methods: A total of 1282 NG isolates were consecutively collected between 2010 and 2017. Patient demographic information was also collected. MICs of ceftriaxone, spectinomycin, ciprofloxacin, azithromycin and penicillin were determined by agar dilution. Isolates were genotyped using N. gonorrhoeae multi-antigen sequence typing (NG-MAST). Results: Among the isolates, 97.4% were resistant to ciprofloxacin and 68.2% to penicillin. Moreover, 5.0% showed decreased susceptibility to ceftriaxone (CROD) and 17.3% were resistant to azithromycin (AZM-R); 1.3% were simultaneously CROD and AZM-R. All isolates were susceptible to spectinomycin. Increasing ceftriaxone MICs were found from 2010 to 2017. A total of 427 sequence types (STs) and 68 genogroups were identified from 724 isolates. ST5061, ST3741 and ST1766 were observed across the study years. ST14638 (n = 3) was predominant among 32 CROD isolates. Prevalent STs were ST5061 (n = 6), ST1866 (n = 5) and ST11133 (n = 5) among 96 AZM-R isolates. Conclusions: A high prevalence of isolates resistant to ciprofloxacin and penicillin was found in this study. Azithromycin, one antimicrobial of dual antimicrobial therapy recommended by the WHO, showed a high prevalence of resistance. The other, ceftriaxone, can be used continuously in this region owing to lower resistance levels. However, the emergence of CROD and decreasing susceptibility to ceftriaxone indicate that continuous antimicrobial resistance surveillance is essential.http://www.sciencedirect.com/science/article/pii/S2213716520302198Neisseria gonorrhoeaeAntimicrobial resistanceCeftriaxoneAzithromycinNG-MAST |
| spellingShingle | Feng Wang Jing-Wei Liu Yi-Zhun Li Li-Jun Zhang Jing Huang Xiang-Sheng Chen Shao-Chun Chen Yue-Ping Yin Surveillance and molecular epidemiology of Neisseria gonorrhoeae isolates in Shenzhen, China, 2010–2017 Journal of Global Antimicrobial Resistance Neisseria gonorrhoeae Antimicrobial resistance Ceftriaxone Azithromycin NG-MAST |
| title | Surveillance and molecular epidemiology of Neisseria gonorrhoeae isolates in Shenzhen, China, 2010–2017 |
| title_full | Surveillance and molecular epidemiology of Neisseria gonorrhoeae isolates in Shenzhen, China, 2010–2017 |
| title_fullStr | Surveillance and molecular epidemiology of Neisseria gonorrhoeae isolates in Shenzhen, China, 2010–2017 |
| title_full_unstemmed | Surveillance and molecular epidemiology of Neisseria gonorrhoeae isolates in Shenzhen, China, 2010–2017 |
| title_short | Surveillance and molecular epidemiology of Neisseria gonorrhoeae isolates in Shenzhen, China, 2010–2017 |
| title_sort | surveillance and molecular epidemiology of neisseria gonorrhoeae isolates in shenzhen china 2010 2017 |
| topic | Neisseria gonorrhoeae Antimicrobial resistance Ceftriaxone Azithromycin NG-MAST |
| url | http://www.sciencedirect.com/science/article/pii/S2213716520302198 |
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