SPR and Double Resonance LPG Biosensors for <i>Helicobacter pylori</i> BabA Antigen Detection
Given the medical and social significance of <i>Helicobacter pylori</i> infection, timely and reliable diagnosis of the disease is required. The traditional invasive and non-invasive conventional diagnostic techniques have several limitations. Recently, opportunities for new diagnostic m...
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MDPI AG
2024-03-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/24/7/2118 |
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| author | Georgi Dyankov Tinko Eftimov Evdokiya Hikova Hristo Najdenski Vesselin Kussovski Petia Genova-Kalou Vihar Mankov Hristo Kisov Petar Veselinov Sanaz Shoar Ghaffari Mila Kovacheva-Slavova Borislav Vladimirov Nikola Malinowski |
| author_facet | Georgi Dyankov Tinko Eftimov Evdokiya Hikova Hristo Najdenski Vesselin Kussovski Petia Genova-Kalou Vihar Mankov Hristo Kisov Petar Veselinov Sanaz Shoar Ghaffari Mila Kovacheva-Slavova Borislav Vladimirov Nikola Malinowski |
| author_sort | Georgi Dyankov |
| collection | DOAJ |
| description | Given the medical and social significance of <i>Helicobacter pylori</i> infection, timely and reliable diagnosis of the disease is required. The traditional invasive and non-invasive conventional diagnostic techniques have several limitations. Recently, opportunities for new diagnostic methods have appeared based on the recent advance in the study of <i>H. pylori</i> outer membrane proteins and their identified receptors. In the present study we assess the way in which outer membrane protein–cell receptor reactions are applicable in establishing a reliable diagnosis. Herein, as well as in other previous studies of ours, we explore the reliability of the binding reaction between the best characterized <i>H. pylori</i> adhesin BabA and its receptor, the blood antigen Le<sup>b</sup>. For the purpose we developed surface plasmon resonance (SPR) and double resonance long period grating (DR LPG) biosensors based on the BabA–Le<sup>b</sup> binding reaction for diagnosing <i>H. pylori</i> infection. In SPR detection, the sensitivity was estimated at 3000 CFU/mL—a much higher sensitivity than that of the RUT test. The DR LPG biosensor proved to be superior in terms of accuracy and sensitivity—concentrations as low as 10<sup>2</sup> CFU/mL were detected. |
| first_indexed | 2024-04-24T10:34:56Z |
| format | Article |
| id | doaj.art-c61d9f17642e4713823b43fcc5e8ac75 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-24T10:34:56Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-c61d9f17642e4713823b43fcc5e8ac752024-04-12T13:26:15ZengMDPI AGSensors1424-82202024-03-01247211810.3390/s24072118SPR and Double Resonance LPG Biosensors for <i>Helicobacter pylori</i> BabA Antigen DetectionGeorgi Dyankov0Tinko Eftimov1Evdokiya Hikova2Hristo Najdenski3Vesselin Kussovski4Petia Genova-Kalou5Vihar Mankov6Hristo Kisov7Petar Veselinov8Sanaz Shoar Ghaffari9Mila Kovacheva-Slavova10Borislav Vladimirov11Nikola Malinowski12Institute of Optical Materials and Technologies “Acad. J. Malinowski” (IOMT), Bulgarian Academy of Sciences (BAS), 109 “Acad. G. Bonchev” Str., 1113 Sofia, BulgariaCentral Laboratory of Applied Physics, Bulgarian Academy of Sciences, 61 Sankt Petersburg Blvd., 4000 Plovdiv, BulgariaInstitute of Optical Materials and Technologies “Acad. J. Malinowski” (IOMT), Bulgarian Academy of Sciences (BAS), 109 “Acad. G. Bonchev” Str., 1113 Sofia, BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 Acad. G. Bonchev Str., 1113 Sofia, BulgariaThe Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 Acad. G. Bonchev Str., 1113 Sofia, BulgariaNational Center of Infectious and Parasitic Diseases, 44A “Gen. Stoletov” Blvd., 1233 Sofia, BulgariaInstitute of Optical Materials and Technologies “Acad. J. Malinowski” (IOMT), Bulgarian Academy of Sciences (BAS), 109 “Acad. G. Bonchev” Str., 1113 Sofia, BulgariaInstitute of Optical Materials and Technologies “Acad. J. Malinowski” (IOMT), Bulgarian Academy of Sciences (BAS), 109 “Acad. G. Bonchev” Str., 1113 Sofia, BulgariaInstitute of Optical Materials and Technologies “Acad. J. Malinowski” (IOMT), Bulgarian Academy of Sciences (BAS), 109 “Acad. G. Bonchev” Str., 1113 Sofia, BulgariaPhotonics Research Center, Université du Québec en Outaouais, Rue 101 St-Jean Bosco, Gatineau, QC J8X 3G5, CanadaDepartment of Gastroenterology, University Hospital Tsaritsa Ioanna-ISUL, Medical University Sofia, 8 “Byalo More” Str., 1527 Sofia, BulgariaDepartment of Gastroenterology, University Hospital Tsaritsa Ioanna-ISUL, Medical University Sofia, 8 “Byalo More” Str., 1527 Sofia, BulgariaInstitute of Optical Materials and Technologies “Acad. J. Malinowski” (IOMT), Bulgarian Academy of Sciences (BAS), 109 “Acad. G. Bonchev” Str., 1113 Sofia, BulgariaGiven the medical and social significance of <i>Helicobacter pylori</i> infection, timely and reliable diagnosis of the disease is required. The traditional invasive and non-invasive conventional diagnostic techniques have several limitations. Recently, opportunities for new diagnostic methods have appeared based on the recent advance in the study of <i>H. pylori</i> outer membrane proteins and their identified receptors. In the present study we assess the way in which outer membrane protein–cell receptor reactions are applicable in establishing a reliable diagnosis. Herein, as well as in other previous studies of ours, we explore the reliability of the binding reaction between the best characterized <i>H. pylori</i> adhesin BabA and its receptor, the blood antigen Le<sup>b</sup>. For the purpose we developed surface plasmon resonance (SPR) and double resonance long period grating (DR LPG) biosensors based on the BabA–Le<sup>b</sup> binding reaction for diagnosing <i>H. pylori</i> infection. In SPR detection, the sensitivity was estimated at 3000 CFU/mL—a much higher sensitivity than that of the RUT test. The DR LPG biosensor proved to be superior in terms of accuracy and sensitivity—concentrations as low as 10<sup>2</sup> CFU/mL were detected.https://www.mdpi.com/1424-8220/24/7/2118<i>Helicobacter pylori</i> diagnosisouter membrane protein BabAblood antigensurface plasmon resonance (SPR)double resonance long period grating (DR LPG)biosensors |
| spellingShingle | Georgi Dyankov Tinko Eftimov Evdokiya Hikova Hristo Najdenski Vesselin Kussovski Petia Genova-Kalou Vihar Mankov Hristo Kisov Petar Veselinov Sanaz Shoar Ghaffari Mila Kovacheva-Slavova Borislav Vladimirov Nikola Malinowski SPR and Double Resonance LPG Biosensors for <i>Helicobacter pylori</i> BabA Antigen Detection Sensors <i>Helicobacter pylori</i> diagnosis outer membrane protein BabA blood antigen surface plasmon resonance (SPR) double resonance long period grating (DR LPG) biosensors |
| title | SPR and Double Resonance LPG Biosensors for <i>Helicobacter pylori</i> BabA Antigen Detection |
| title_full | SPR and Double Resonance LPG Biosensors for <i>Helicobacter pylori</i> BabA Antigen Detection |
| title_fullStr | SPR and Double Resonance LPG Biosensors for <i>Helicobacter pylori</i> BabA Antigen Detection |
| title_full_unstemmed | SPR and Double Resonance LPG Biosensors for <i>Helicobacter pylori</i> BabA Antigen Detection |
| title_short | SPR and Double Resonance LPG Biosensors for <i>Helicobacter pylori</i> BabA Antigen Detection |
| title_sort | spr and double resonance lpg biosensors for i helicobacter pylori i baba antigen detection |
| topic | <i>Helicobacter pylori</i> diagnosis outer membrane protein BabA blood antigen surface plasmon resonance (SPR) double resonance long period grating (DR LPG) biosensors |
| url | https://www.mdpi.com/1424-8220/24/7/2118 |
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