Characterization of C30 carotenoid and identification of its biosynthetic gene cluster in Methylobacterium extorquens AM1
Methylobacterium species, the representative bacteria distributed in phyllosphere region of plants, often synthesize carotenoids to resist harmful UV radiations. Methylobacterium extorquens is known to produce a carotenoid pigment and recent research revealed that this carotenoid has a C30 backbone....
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2023-09-01
|
| Series: | Synthetic and Systems Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405805X23000595 |
| _version_ | 1827267043307552768 |
|---|---|
| author | Xu-Hua Mo Yu-Man Sun Yu-Xing Bi Yan Zhao Gui-Hong Yu Ling-ling Tan Song Yang |
| author_facet | Xu-Hua Mo Yu-Man Sun Yu-Xing Bi Yan Zhao Gui-Hong Yu Ling-ling Tan Song Yang |
| author_sort | Xu-Hua Mo |
| collection | DOAJ |
| description | Methylobacterium species, the representative bacteria distributed in phyllosphere region of plants, often synthesize carotenoids to resist harmful UV radiations. Methylobacterium extorquens is known to produce a carotenoid pigment and recent research revealed that this carotenoid has a C30 backbone. However, its exact structure remains unknown. In the present study, the carotenoid produced by M. extorquens AM1 was isolated and its structure was determined as 4-[2-O-11Z-octadecenoyl-β-glucopyranosyl]-4,4′-diapolycopenedioc acid (1), a glycosylated C30 carotenoid. Furthermore, the genes related to the C30 carotenoid synthesis were investigated. Squalene, the precursor of the C30 carotenoid, is synthesized by the co-occurrence of META1p1815, META1p1816 and META1p1817. Further overexpression of the genes related to squalene synthesis improved the titer of carotenoid 1. By using gene deletion and gene complementation experiments, the glycosyltransferase META1p3663 and acyltransferase META1p3664 were firstly confirmed to catalyze the tailoring steps from 4,4′-diapolycopene-4,4′-dioic acid to carotenoid 1. In conclusion, the structure and biosynthetic genes of carotenoid 1 produced by M. extorquens AM1 were firstly characterized in this work, which shed lights on engineering M. extorquens AM1 for producing carotenoid 1 in high yield. |
| first_indexed | 2024-03-12T11:02:52Z |
| format | Article |
| id | doaj.art-c61ea116f2234b198607879da2692d4a |
| institution | Directory Open Access Journal |
| issn | 2405-805X |
| language | English |
| last_indexed | 2025-03-22T04:27:28Z |
| publishDate | 2023-09-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Synthetic and Systems Biotechnology |
| spelling | doaj.art-c61ea116f2234b198607879da2692d4a2024-04-28T05:28:10ZengKeAi Communications Co., Ltd.Synthetic and Systems Biotechnology2405-805X2023-09-0183527535Characterization of C30 carotenoid and identification of its biosynthetic gene cluster in Methylobacterium extorquens AM1Xu-Hua Mo0Yu-Man Sun1Yu-Xing Bi2Yan Zhao3Gui-Hong Yu4Ling-ling Tan5Song Yang6School of Life Sciences, Qingdao Agricultural University, 700 Changcheng Road, Qingdao, Shandong, 266109, China; Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural University, 700 Changcheng Road, Qingdao, Shandong, 266109, China; Qingdao International Center on Microbes Utilizing Biogas, Qingdao Agricultural University, Qingdao, Shandong Province, ChinaSchool of Life Sciences, Qingdao Agricultural University, 700 Changcheng Road, Qingdao, Shandong, 266109, China; Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural University, 700 Changcheng Road, Qingdao, Shandong, 266109, China; Qingdao International Center on Microbes Utilizing Biogas, Qingdao Agricultural University, Qingdao, Shandong Province, ChinaSchool of Life Sciences, Qingdao Agricultural University, 700 Changcheng Road, Qingdao, Shandong, 266109, China; Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural University, 700 Changcheng Road, Qingdao, Shandong, 266109, China; Qingdao International Center on Microbes Utilizing Biogas, Qingdao Agricultural University, Qingdao, Shandong Province, ChinaSchool of Life Sciences, Qingdao Agricultural University, 700 Changcheng Road, Qingdao, Shandong, 266109, China; Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural University, 700 Changcheng Road, Qingdao, Shandong, 266109, China; Qingdao International Center on Microbes Utilizing Biogas, Qingdao Agricultural University, Qingdao, Shandong Province, ChinaSchool of Life Sciences, Qingdao Agricultural University, 700 Changcheng Road, Qingdao, Shandong, 266109, China; Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural University, 700 Changcheng Road, Qingdao, Shandong, 266109, China; Qingdao International Center on Microbes Utilizing Biogas, Qingdao Agricultural University, Qingdao, Shandong Province, ChinaSchool of Life Sciences, Qingdao Agricultural University, 700 Changcheng Road, Qingdao, Shandong, 266109, China; Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural University, 700 Changcheng Road, Qingdao, Shandong, 266109, China; Qingdao International Center on Microbes Utilizing Biogas, Qingdao Agricultural University, Qingdao, Shandong Province, China; Corresponding author. School of Life Sciences, Qingdao Agricultural University, 700 Changcheng Road, Qingdao, Shandong, 266109, China.School of Life Sciences, Qingdao Agricultural University, 700 Changcheng Road, Qingdao, Shandong, 266109, China; Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural University, 700 Changcheng Road, Qingdao, Shandong, 266109, China; Qingdao International Center on Microbes Utilizing Biogas, Qingdao Agricultural University, Qingdao, Shandong Province, China; Corresponding author. School of Life Sciences, Qingdao Agricultural University, 700 Changcheng Road, Qingdao, Shandong, 266109, China.Methylobacterium species, the representative bacteria distributed in phyllosphere region of plants, often synthesize carotenoids to resist harmful UV radiations. Methylobacterium extorquens is known to produce a carotenoid pigment and recent research revealed that this carotenoid has a C30 backbone. However, its exact structure remains unknown. In the present study, the carotenoid produced by M. extorquens AM1 was isolated and its structure was determined as 4-[2-O-11Z-octadecenoyl-β-glucopyranosyl]-4,4′-diapolycopenedioc acid (1), a glycosylated C30 carotenoid. Furthermore, the genes related to the C30 carotenoid synthesis were investigated. Squalene, the precursor of the C30 carotenoid, is synthesized by the co-occurrence of META1p1815, META1p1816 and META1p1817. Further overexpression of the genes related to squalene synthesis improved the titer of carotenoid 1. By using gene deletion and gene complementation experiments, the glycosyltransferase META1p3663 and acyltransferase META1p3664 were firstly confirmed to catalyze the tailoring steps from 4,4′-diapolycopene-4,4′-dioic acid to carotenoid 1. In conclusion, the structure and biosynthetic genes of carotenoid 1 produced by M. extorquens AM1 were firstly characterized in this work, which shed lights on engineering M. extorquens AM1 for producing carotenoid 1 in high yield.http://www.sciencedirect.com/science/article/pii/S2405805X23000595C30 carotenoidMethylobacterium extorquensBiosynthetic gene clusterGlycosyltransferaseAcyltransferase |
| spellingShingle | Xu-Hua Mo Yu-Man Sun Yu-Xing Bi Yan Zhao Gui-Hong Yu Ling-ling Tan Song Yang Characterization of C30 carotenoid and identification of its biosynthetic gene cluster in Methylobacterium extorquens AM1 Synthetic and Systems Biotechnology C30 carotenoid Methylobacterium extorquens Biosynthetic gene cluster Glycosyltransferase Acyltransferase |
| title | Characterization of C30 carotenoid and identification of its biosynthetic gene cluster in Methylobacterium extorquens AM1 |
| title_full | Characterization of C30 carotenoid and identification of its biosynthetic gene cluster in Methylobacterium extorquens AM1 |
| title_fullStr | Characterization of C30 carotenoid and identification of its biosynthetic gene cluster in Methylobacterium extorquens AM1 |
| title_full_unstemmed | Characterization of C30 carotenoid and identification of its biosynthetic gene cluster in Methylobacterium extorquens AM1 |
| title_short | Characterization of C30 carotenoid and identification of its biosynthetic gene cluster in Methylobacterium extorquens AM1 |
| title_sort | characterization of c30 carotenoid and identification of its biosynthetic gene cluster in methylobacterium extorquens am1 |
| topic | C30 carotenoid Methylobacterium extorquens Biosynthetic gene cluster Glycosyltransferase Acyltransferase |
| url | http://www.sciencedirect.com/science/article/pii/S2405805X23000595 |
| work_keys_str_mv | AT xuhuamo characterizationofc30carotenoidandidentificationofitsbiosyntheticgeneclusterinmethylobacteriumextorquensam1 AT yumansun characterizationofc30carotenoidandidentificationofitsbiosyntheticgeneclusterinmethylobacteriumextorquensam1 AT yuxingbi characterizationofc30carotenoidandidentificationofitsbiosyntheticgeneclusterinmethylobacteriumextorquensam1 AT yanzhao characterizationofc30carotenoidandidentificationofitsbiosyntheticgeneclusterinmethylobacteriumextorquensam1 AT guihongyu characterizationofc30carotenoidandidentificationofitsbiosyntheticgeneclusterinmethylobacteriumextorquensam1 AT linglingtan characterizationofc30carotenoidandidentificationofitsbiosyntheticgeneclusterinmethylobacteriumextorquensam1 AT songyang characterizationofc30carotenoidandidentificationofitsbiosyntheticgeneclusterinmethylobacteriumextorquensam1 |