Kinetic Study of 17α-Estradiol Mechanism during Rat Sperm Capacitation
17α-Estradiol (αE2) is a natural diastereoisomer of 17β-estradiol (E2). It is well known that αE2 can bind to estrogen receptors. However, its biological activity is less than that of E2 and is species and tissue specific. The goal of our study was to propose the mechanism of αE2 hormonal response i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/13/4092 |
| _version_ | 1827619389829021696 |
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| author | Tereza Bosakova Antonin Tockstein Zuzana Bosakova Katerina Komrskova |
| author_facet | Tereza Bosakova Antonin Tockstein Zuzana Bosakova Katerina Komrskova |
| author_sort | Tereza Bosakova |
| collection | DOAJ |
| description | 17α-Estradiol (αE2) is a natural diastereoisomer of 17β-estradiol (E2). It is well known that αE2 can bind to estrogen receptors. However, its biological activity is less than that of E2 and is species and tissue specific. The goal of our study was to propose the mechanism of αE2 hormonal response in rat sperm during their capacitation in vitro and compare it with a previously studied mouse model. Concentration changes in externally added αE2 during capacitation of rat sperm were monitored by the high-performance liquid chromatographic method with tandem mass spectrometric detection (HPLC-MS/MS). The calculated values of relative concentrations <i>B</i><sub>t</sub> were subjected to kinetic analysis. The findings indicated that αE2 in rat sperm did not trigger autocatalytic reaction, in contrast to the mouse sperm, and that the initiation of the hormone penetration through the sperm plasma membrane was substantially faster in rats. |
| first_indexed | 2024-03-09T10:27:32Z |
| format | Article |
| id | doaj.art-c61f40dc8e76419d9fe65fd183e66c55 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T10:27:32Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c61f40dc8e76419d9fe65fd183e66c552023-12-01T21:35:58ZengMDPI AGMolecules1420-30492022-06-012713409210.3390/molecules27134092Kinetic Study of 17α-Estradiol Mechanism during Rat Sperm CapacitationTereza Bosakova0Antonin Tockstein1Zuzana Bosakova2Katerina Komrskova3Department of Analytical Chemistry, Faculty of Science, Charles University, Albertov 2030, Prague 2, 128 43 Prague, Czech RepublicDepartment of Analytical Chemistry, Faculty of Science, Charles University, Albertov 2030, Prague 2, 128 43 Prague, Czech RepublicDepartment of Analytical Chemistry, Faculty of Science, Charles University, Albertov 2030, Prague 2, 128 43 Prague, Czech RepublicLaboratory of Reproductive Biology, Institute of Biotechnology, Czech Academy of Sciences, BIOCEV, Prumyslova 595, 252 50 Vestec, Czech Republic17α-Estradiol (αE2) is a natural diastereoisomer of 17β-estradiol (E2). It is well known that αE2 can bind to estrogen receptors. However, its biological activity is less than that of E2 and is species and tissue specific. The goal of our study was to propose the mechanism of αE2 hormonal response in rat sperm during their capacitation in vitro and compare it with a previously studied mouse model. Concentration changes in externally added αE2 during capacitation of rat sperm were monitored by the high-performance liquid chromatographic method with tandem mass spectrometric detection (HPLC-MS/MS). The calculated values of relative concentrations <i>B</i><sub>t</sub> were subjected to kinetic analysis. The findings indicated that αE2 in rat sperm did not trigger autocatalytic reaction, in contrast to the mouse sperm, and that the initiation of the hormone penetration through the sperm plasma membrane was substantially faster in rats.https://www.mdpi.com/1420-3049/27/13/409217α-estradiolestrogen receptorsratspermcapacitationHPLC MS/MS |
| spellingShingle | Tereza Bosakova Antonin Tockstein Zuzana Bosakova Katerina Komrskova Kinetic Study of 17α-Estradiol Mechanism during Rat Sperm Capacitation Molecules 17α-estradiol estrogen receptors rat sperm capacitation HPLC MS/MS |
| title | Kinetic Study of 17α-Estradiol Mechanism during Rat Sperm Capacitation |
| title_full | Kinetic Study of 17α-Estradiol Mechanism during Rat Sperm Capacitation |
| title_fullStr | Kinetic Study of 17α-Estradiol Mechanism during Rat Sperm Capacitation |
| title_full_unstemmed | Kinetic Study of 17α-Estradiol Mechanism during Rat Sperm Capacitation |
| title_short | Kinetic Study of 17α-Estradiol Mechanism during Rat Sperm Capacitation |
| title_sort | kinetic study of 17α estradiol mechanism during rat sperm capacitation |
| topic | 17α-estradiol estrogen receptors rat sperm capacitation HPLC MS/MS |
| url | https://www.mdpi.com/1420-3049/27/13/4092 |
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