Reproducible reformation of a bilayer lipid membrane using microair bubbles
Abstract Planar bilayer lipid membranes (BLMs) are widely used as models for cell membranes in various applications, including drug discovery and biosensors. However, the nanometer‐thick bilayer structure, assembled through hydrophobic interactions of amphiphilic lipid molecules, makes such BLM syst...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-07-01
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| Series: | Droplet |
| Online Access: | https://doi.org/10.1002/dro2.73 |
| _version_ | 1797516083188465664 |
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| author | Izumi Hashimoto Toshihisa Osaki Hirotaka Sugiura Hisatoshi Mimura Sho Takamori Norihisa Miki Shoji Takeuchi |
| author_facet | Izumi Hashimoto Toshihisa Osaki Hirotaka Sugiura Hisatoshi Mimura Sho Takamori Norihisa Miki Shoji Takeuchi |
| author_sort | Izumi Hashimoto |
| collection | DOAJ |
| description | Abstract Planar bilayer lipid membranes (BLMs) are widely used as models for cell membranes in various applications, including drug discovery and biosensors. However, the nanometer‐thick bilayer structure, assembled through hydrophobic interactions of amphiphilic lipid molecules, makes such BLM systems mechanically and electrically unstable. In this study, we developed a device to reform BLMs using a microair bubble. The device consists of a double well divided by a separator with a microaperture, where a BLM was formed by infusing a lipid‐dispersed solvent and an aqueous droplet into each well in series. When the BLM ruptured, a microair bubble was injected from the bottom of the well to split the merged aqueous droplet at the microaperture, which resulted in the reformation of two lipid monolayers on the split droplets. By bringing the two droplets into contact, a new BLM was formed. An angled step design was introduced in the BLM device to guide the bubble and ensure the splitting of the merged droplet. We also elucidated the optimal bubble inflow rate for the reproducible BLM reformation. Using a 4‐channel parallel device, we demonstrated the individual and repeatable reformation of BLMs. Our approach will aid the development of automated and arrayed BLM systems. |
| first_indexed | 2024-03-10T06:56:19Z |
| format | Article |
| id | doaj.art-c621641fc4304ecf80f4f746750d730c |
| institution | Directory Open Access Journal |
| issn | 2731-4375 |
| language | English |
| last_indexed | 2024-03-10T06:56:19Z |
| publishDate | 2023-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Droplet |
| spelling | doaj.art-c621641fc4304ecf80f4f746750d730c2023-11-22T16:25:33ZengWileyDroplet2731-43752023-07-0123n/an/a10.1002/dro2.73Reproducible reformation of a bilayer lipid membrane using microair bubblesIzumi Hashimoto0Toshihisa Osaki1Hirotaka Sugiura2Hisatoshi Mimura3Sho Takamori4Norihisa Miki5Shoji Takeuchi6Artificial Cell Membrane Systems Group Kanagawa Institute of Industrial Science and Technology Kanagawa JapanArtificial Cell Membrane Systems Group Kanagawa Institute of Industrial Science and Technology Kanagawa JapanArtificial Cell Membrane Systems Group Kanagawa Institute of Industrial Science and Technology Kanagawa JapanArtificial Cell Membrane Systems Group Kanagawa Institute of Industrial Science and Technology Kanagawa JapanArtificial Cell Membrane Systems Group Kanagawa Institute of Industrial Science and Technology Kanagawa JapanArtificial Cell Membrane Systems Group Kanagawa Institute of Industrial Science and Technology Kanagawa JapanArtificial Cell Membrane Systems Group Kanagawa Institute of Industrial Science and Technology Kanagawa JapanAbstract Planar bilayer lipid membranes (BLMs) are widely used as models for cell membranes in various applications, including drug discovery and biosensors. However, the nanometer‐thick bilayer structure, assembled through hydrophobic interactions of amphiphilic lipid molecules, makes such BLM systems mechanically and electrically unstable. In this study, we developed a device to reform BLMs using a microair bubble. The device consists of a double well divided by a separator with a microaperture, where a BLM was formed by infusing a lipid‐dispersed solvent and an aqueous droplet into each well in series. When the BLM ruptured, a microair bubble was injected from the bottom of the well to split the merged aqueous droplet at the microaperture, which resulted in the reformation of two lipid monolayers on the split droplets. By bringing the two droplets into contact, a new BLM was formed. An angled step design was introduced in the BLM device to guide the bubble and ensure the splitting of the merged droplet. We also elucidated the optimal bubble inflow rate for the reproducible BLM reformation. Using a 4‐channel parallel device, we demonstrated the individual and repeatable reformation of BLMs. Our approach will aid the development of automated and arrayed BLM systems.https://doi.org/10.1002/dro2.73 |
| spellingShingle | Izumi Hashimoto Toshihisa Osaki Hirotaka Sugiura Hisatoshi Mimura Sho Takamori Norihisa Miki Shoji Takeuchi Reproducible reformation of a bilayer lipid membrane using microair bubbles Droplet |
| title | Reproducible reformation of a bilayer lipid membrane using microair bubbles |
| title_full | Reproducible reformation of a bilayer lipid membrane using microair bubbles |
| title_fullStr | Reproducible reformation of a bilayer lipid membrane using microair bubbles |
| title_full_unstemmed | Reproducible reformation of a bilayer lipid membrane using microair bubbles |
| title_short | Reproducible reformation of a bilayer lipid membrane using microair bubbles |
| title_sort | reproducible reformation of a bilayer lipid membrane using microair bubbles |
| url | https://doi.org/10.1002/dro2.73 |
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