Electroacupuncture activates angiogenesis by regulating the PI3K/Pten/Thbs1 signaling pathway to promote the browning of adipose tissue in HFD-induced obese mice
This study investigated the effect of electroacupuncture (EA) on the browning of white adipose tissue (WAT) via angiogenesis and its potential mechanism in obese mice. Four-week-old male C56BL/6 mice were randomly divided into a high-fat diet (HFD) and a normal chow diet (ND) group. After 12 weeks,...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Biomedicine & Pharmacotherapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332223011836 |
| _version_ | 1797686889674702848 |
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| author | Hongyan Gao Yanhui Li Yue Jin Li Zhang Xiuwen Xia Jinkun Liu Huaifu Wang Ya Xie Weijun Ding |
| author_facet | Hongyan Gao Yanhui Li Yue Jin Li Zhang Xiuwen Xia Jinkun Liu Huaifu Wang Ya Xie Weijun Ding |
| author_sort | Hongyan Gao |
| collection | DOAJ |
| description | This study investigated the effect of electroacupuncture (EA) on the browning of white adipose tissue (WAT) via angiogenesis and its potential mechanism in obese mice. Four-week-old male C56BL/6 mice were randomly divided into a high-fat diet (HFD) and a normal chow diet (ND) group. After 12 weeks, HFD mice were randomly divided into two groups to receive or not receive EA for 3 weeks. After EA treatment, body weight, adipocyte size, serum glucose (GLU), triacylglycerol (TG), cholesterol (CHO), leptin (Lep), monocyte chemoattractant protein-1 (MCP-1), WAT browning-related genes, angiogenesis-related genes, and the PI3K/Pten/Thbs1 signaling pathway were evaluated. The results indicated that EA significantly reduced body weight, adipocyte size, and serum concentrations of GLU, TG, CHO, Lep and MCP-1 and promoted WAT browning. Angiogenesis and the PI3K/Pten/Thbs1 signaling pathway were all activated by EA intervention. The expression levels were consistent with the results of RNA-seq and confirmed via qRTPCR and WB. Our study showed that EA may activate angiogenesis via the PI3K/Pten/Thbs1 signaling pathway in WAT, thereby promoting the browning and thermogenesis of adipose tissue. |
| first_indexed | 2024-03-12T01:11:06Z |
| format | Article |
| id | doaj.art-c621e275e54840a283af5cdcbd97c6a1 |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-03-12T01:11:06Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-c621e275e54840a283af5cdcbd97c6a12023-09-14T04:52:52ZengElsevierBiomedicine & Pharmacotherapy0753-33222023-10-01166115386Electroacupuncture activates angiogenesis by regulating the PI3K/Pten/Thbs1 signaling pathway to promote the browning of adipose tissue in HFD-induced obese miceHongyan Gao0Yanhui Li1Yue Jin2Li Zhang3Xiuwen Xia4Jinkun Liu5Huaifu Wang6Ya Xie7Weijun Ding8School of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaChongqing Hospital of Traditional Chinese Medicine, Chongqing 400021, ChinaSchool of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaSchool of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaSchool of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaChongqing Hospital of Traditional Chinese Medicine, Chongqing 400021, ChinaSchool of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaSichuan Provincial People’s Hospital Jinniu Hospital, Chengdu 610007, ChinaSchool of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China; Correspondence to: School of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, 1166 Liutai Avenue, Chengdu 611137, PR China.This study investigated the effect of electroacupuncture (EA) on the browning of white adipose tissue (WAT) via angiogenesis and its potential mechanism in obese mice. Four-week-old male C56BL/6 mice were randomly divided into a high-fat diet (HFD) and a normal chow diet (ND) group. After 12 weeks, HFD mice were randomly divided into two groups to receive or not receive EA for 3 weeks. After EA treatment, body weight, adipocyte size, serum glucose (GLU), triacylglycerol (TG), cholesterol (CHO), leptin (Lep), monocyte chemoattractant protein-1 (MCP-1), WAT browning-related genes, angiogenesis-related genes, and the PI3K/Pten/Thbs1 signaling pathway were evaluated. The results indicated that EA significantly reduced body weight, adipocyte size, and serum concentrations of GLU, TG, CHO, Lep and MCP-1 and promoted WAT browning. Angiogenesis and the PI3K/Pten/Thbs1 signaling pathway were all activated by EA intervention. The expression levels were consistent with the results of RNA-seq and confirmed via qRTPCR and WB. Our study showed that EA may activate angiogenesis via the PI3K/Pten/Thbs1 signaling pathway in WAT, thereby promoting the browning and thermogenesis of adipose tissue.http://www.sciencedirect.com/science/article/pii/S0753332223011836Electroacupuncture (EA)White adipose tissue (WAT)BrowningAngiogenesisObesity |
| spellingShingle | Hongyan Gao Yanhui Li Yue Jin Li Zhang Xiuwen Xia Jinkun Liu Huaifu Wang Ya Xie Weijun Ding Electroacupuncture activates angiogenesis by regulating the PI3K/Pten/Thbs1 signaling pathway to promote the browning of adipose tissue in HFD-induced obese mice Biomedicine & Pharmacotherapy Electroacupuncture (EA) White adipose tissue (WAT) Browning Angiogenesis Obesity |
| title | Electroacupuncture activates angiogenesis by regulating the PI3K/Pten/Thbs1 signaling pathway to promote the browning of adipose tissue in HFD-induced obese mice |
| title_full | Electroacupuncture activates angiogenesis by regulating the PI3K/Pten/Thbs1 signaling pathway to promote the browning of adipose tissue in HFD-induced obese mice |
| title_fullStr | Electroacupuncture activates angiogenesis by regulating the PI3K/Pten/Thbs1 signaling pathway to promote the browning of adipose tissue in HFD-induced obese mice |
| title_full_unstemmed | Electroacupuncture activates angiogenesis by regulating the PI3K/Pten/Thbs1 signaling pathway to promote the browning of adipose tissue in HFD-induced obese mice |
| title_short | Electroacupuncture activates angiogenesis by regulating the PI3K/Pten/Thbs1 signaling pathway to promote the browning of adipose tissue in HFD-induced obese mice |
| title_sort | electroacupuncture activates angiogenesis by regulating the pi3k pten thbs1 signaling pathway to promote the browning of adipose tissue in hfd induced obese mice |
| topic | Electroacupuncture (EA) White adipose tissue (WAT) Browning Angiogenesis Obesity |
| url | http://www.sciencedirect.com/science/article/pii/S0753332223011836 |
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