Analysis of virulence factors of <it>Helicobacter pylori </it>isolated from a Vietnamese population
<p>Abstract</p> <p>Background</p> <p>The incidence of gastric cancer differs among countries in Asia, and it has been suggested that virulence factors associated with <it>Helicobacter pylori </it>are partly responsible. The aim of this study was to investiga...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2009-08-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/9/175 |
| _version_ | 1818348932073259008 |
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| author | Ta Long Trinh Tuan Matsuhisa Takeshi Murakami Kazunari Kodama Masaaki Okimoto Tadayoshi Takayama Akiko Nguyen Lam Uchida Tomohisa Ho Dang Hoang Hoa Kishida Tetsuko Fujioka Toshio Moriyama Masatsugu Yamaoka Yoshio |
| author_facet | Ta Long Trinh Tuan Matsuhisa Takeshi Murakami Kazunari Kodama Masaaki Okimoto Tadayoshi Takayama Akiko Nguyen Lam Uchida Tomohisa Ho Dang Hoang Hoa Kishida Tetsuko Fujioka Toshio Moriyama Masatsugu Yamaoka Yoshio |
| author_sort | Ta Long |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The incidence of gastric cancer differs among countries in Asia, and it has been suggested that virulence factors associated with <it>Helicobacter pylori </it>are partly responsible. The aim of this study was to investigate several genetic factors regarded as virulence or molecular epidemiologic markers in <it>H. pylori </it>isolates from Vietnamese subjects.</p> <p>Results</p> <p>The <it>cagA</it>, <it>vacA </it>and <it>cag </it>right-end junction genotypes of 103 <it>H. pylori </it>strains from Vietnam (54 from Hanoi and 49 from Ho Chi Minh) were determined by PCR and sequencing. Three types of deletion in the region located upstream of the <it>cagA </it>Glu-Pro-Ile-Tyr-Ala (EPIYA) repeat region were identified: the 39-bp deletion type, the 18-bp deletion type, and the no-deletion type. The majority of strains studied (77%; 80/103) had the 18-bp deletion irrespective of geographical location in the country or clinical outcome. All of the 39-bp and 18-bp deletion-type strains possessed the East Asian type <it>cagA </it>repeat region. The type II <it>cag </it>right-end junction genotype was predominant (84%). The <it>vacA </it>m1 genotype was significantly more common in strains isolated in Hanoi, where the incidence of gastric cancer is higher, than in strains from Ho Chi Minh.</p> <p>Conclusion</p> <p>Pre-EPIYA-region typing of the <it>cagA </it>gene could provide a new genetic marker of <it>H. pylori </it>genomic diversity. Our data support the hypothesis that <it>vacA </it>m1 is closely associated with gastric carcinogenesis.</p> |
| first_indexed | 2024-12-13T17:57:54Z |
| format | Article |
| id | doaj.art-c625e00bfb8241a688bfbfaf93f37fc2 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-13T17:57:54Z |
| publishDate | 2009-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-c625e00bfb8241a688bfbfaf93f37fc22022-12-21T23:36:20ZengBMCBMC Microbiology1471-21802009-08-019117510.1186/1471-2180-9-175Analysis of virulence factors of <it>Helicobacter pylori </it>isolated from a Vietnamese populationTa LongTrinh TuanMatsuhisa TakeshiMurakami KazunariKodama MasaakiOkimoto TadayoshiTakayama AkikoNguyen LamUchida TomohisaHo DangHoang HoaKishida TetsukoFujioka ToshioMoriyama MasatsuguYamaoka Yoshio<p>Abstract</p> <p>Background</p> <p>The incidence of gastric cancer differs among countries in Asia, and it has been suggested that virulence factors associated with <it>Helicobacter pylori </it>are partly responsible. The aim of this study was to investigate several genetic factors regarded as virulence or molecular epidemiologic markers in <it>H. pylori </it>isolates from Vietnamese subjects.</p> <p>Results</p> <p>The <it>cagA</it>, <it>vacA </it>and <it>cag </it>right-end junction genotypes of 103 <it>H. pylori </it>strains from Vietnam (54 from Hanoi and 49 from Ho Chi Minh) were determined by PCR and sequencing. Three types of deletion in the region located upstream of the <it>cagA </it>Glu-Pro-Ile-Tyr-Ala (EPIYA) repeat region were identified: the 39-bp deletion type, the 18-bp deletion type, and the no-deletion type. The majority of strains studied (77%; 80/103) had the 18-bp deletion irrespective of geographical location in the country or clinical outcome. All of the 39-bp and 18-bp deletion-type strains possessed the East Asian type <it>cagA </it>repeat region. The type II <it>cag </it>right-end junction genotype was predominant (84%). The <it>vacA </it>m1 genotype was significantly more common in strains isolated in Hanoi, where the incidence of gastric cancer is higher, than in strains from Ho Chi Minh.</p> <p>Conclusion</p> <p>Pre-EPIYA-region typing of the <it>cagA </it>gene could provide a new genetic marker of <it>H. pylori </it>genomic diversity. Our data support the hypothesis that <it>vacA </it>m1 is closely associated with gastric carcinogenesis.</p>http://www.biomedcentral.com/1471-2180/9/175 |
| spellingShingle | Ta Long Trinh Tuan Matsuhisa Takeshi Murakami Kazunari Kodama Masaaki Okimoto Tadayoshi Takayama Akiko Nguyen Lam Uchida Tomohisa Ho Dang Hoang Hoa Kishida Tetsuko Fujioka Toshio Moriyama Masatsugu Yamaoka Yoshio Analysis of virulence factors of <it>Helicobacter pylori </it>isolated from a Vietnamese population BMC Microbiology |
| title | Analysis of virulence factors of <it>Helicobacter pylori </it>isolated from a Vietnamese population |
| title_full | Analysis of virulence factors of <it>Helicobacter pylori </it>isolated from a Vietnamese population |
| title_fullStr | Analysis of virulence factors of <it>Helicobacter pylori </it>isolated from a Vietnamese population |
| title_full_unstemmed | Analysis of virulence factors of <it>Helicobacter pylori </it>isolated from a Vietnamese population |
| title_short | Analysis of virulence factors of <it>Helicobacter pylori </it>isolated from a Vietnamese population |
| title_sort | analysis of virulence factors of it helicobacter pylori it isolated from a vietnamese population |
| url | http://www.biomedcentral.com/1471-2180/9/175 |
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