Fluorescence in situ hybridization analysis of the hTERC region in acute myeloid leukemia patients
OBJECTIVE: The telomerase RNA component (hTERC) gene is located at 3q26. Increased hTERC gene expression has been frequently observed and amplification was shown using fluorescence in situ hybridization (FISH) in different cancers. The aim of this study was to determine whether hTERC gene amplificat...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2011-05-01
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| Series: | Turkish Journal of Hematology |
| Subjects: | |
| Online Access: | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-37167 |
| _version_ | 1797912454696534016 |
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| author | Özge Özer Tuğçe Bulakbaşı Balcı Zerrin Yılmaz Feride İffet Şahin |
| author_facet | Özge Özer Tuğçe Bulakbaşı Balcı Zerrin Yılmaz Feride İffet Şahin |
| author_sort | Özge Özer |
| collection | DOAJ |
| description | OBJECTIVE: The telomerase RNA component (hTERC) gene is located at 3q26. Increased hTERC gene expression has been frequently observed and amplification was shown using fluorescence in situ hybridization (FISH) in different cancers. The aim of this study was to determine whether hTERC gene amplification is detectable by FISH in acute myeloid leukemia (AML) cells. METHODS: FISH and karyotype results at the time of diagnosis of 23 adult AML patients were retrospectively evaluated. Additionally, fixed cells were hybridized with an hTERC region-specific FISH probe to determine gene amplification. RESULTS: Ten of the 23 patients had a normal karyotype and 6 had an abnormal karyotype. hTERC region amplification was not observed in any of the patients. CONCLUSION: Although it was reported that hTERC gene amplification may partially contribute to increased telomerase expression and activity in leukemic cells, it is not possible to make such a conclusion based on the results of the this study, as hTERC amplification was not observed in the study group. This suggests that increased telomerase activity via gene amplification in the development of AML may not be as important a factor as it is in solid tumors. |
| first_indexed | 2024-04-10T11:57:29Z |
| format | Article |
| id | doaj.art-c62b160bfb954be79bf0a534e2fbe674 |
| institution | Directory Open Access Journal |
| issn | 1308-5263 |
| language | English |
| last_indexed | 2024-04-10T11:57:29Z |
| publishDate | 2011-05-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Turkish Journal of Hematology |
| spelling | doaj.art-c62b160bfb954be79bf0a534e2fbe6742023-02-15T16:16:47ZengGalenos Publishing HouseTurkish Journal of Hematology1308-52632011-05-0128210310610.5152/tjh.2011.25TJH-37167Fluorescence in situ hybridization analysis of the hTERC region in acute myeloid leukemia patientsÖzge Özer0Tuğçe Bulakbaşı Balcı1Zerrin Yılmaz2Feride İffet Şahin3Department Of Medical Genetics, Faculty Of Medicine, Başkent University, Ankara, TurkeyDepartment Of Medical Genetics, Faculty Of Medicine, Başkent University, Ankara, TurkeyDepartment Of Medical Genetics, Faculty Of Medicine, Başkent University, Ankara, TurkeyDepartment Of Medical Genetics, Faculty Of Medicine, Başkent University, Ankara, TurkeyOBJECTIVE: The telomerase RNA component (hTERC) gene is located at 3q26. Increased hTERC gene expression has been frequently observed and amplification was shown using fluorescence in situ hybridization (FISH) in different cancers. The aim of this study was to determine whether hTERC gene amplification is detectable by FISH in acute myeloid leukemia (AML) cells. METHODS: FISH and karyotype results at the time of diagnosis of 23 adult AML patients were retrospectively evaluated. Additionally, fixed cells were hybridized with an hTERC region-specific FISH probe to determine gene amplification. RESULTS: Ten of the 23 patients had a normal karyotype and 6 had an abnormal karyotype. hTERC region amplification was not observed in any of the patients. CONCLUSION: Although it was reported that hTERC gene amplification may partially contribute to increased telomerase expression and activity in leukemic cells, it is not possible to make such a conclusion based on the results of the this study, as hTERC amplification was not observed in the study group. This suggests that increased telomerase activity via gene amplification in the development of AML may not be as important a factor as it is in solid tumors.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-37167amlhtercfish |
| spellingShingle | Özge Özer Tuğçe Bulakbaşı Balcı Zerrin Yılmaz Feride İffet Şahin Fluorescence in situ hybridization analysis of the hTERC region in acute myeloid leukemia patients Turkish Journal of Hematology aml hterc fish |
| title | Fluorescence in situ hybridization analysis of the hTERC region in acute myeloid leukemia patients |
| title_full | Fluorescence in situ hybridization analysis of the hTERC region in acute myeloid leukemia patients |
| title_fullStr | Fluorescence in situ hybridization analysis of the hTERC region in acute myeloid leukemia patients |
| title_full_unstemmed | Fluorescence in situ hybridization analysis of the hTERC region in acute myeloid leukemia patients |
| title_short | Fluorescence in situ hybridization analysis of the hTERC region in acute myeloid leukemia patients |
| title_sort | fluorescence in situ hybridization analysis of the hterc region in acute myeloid leukemia patients |
| topic | aml hterc fish |
| url | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-37167 |
| work_keys_str_mv | AT ozgeozer fluorescenceinsituhybridizationanalysisofthehtercregioninacutemyeloidleukemiapatients AT tugcebulakbasıbalcı fluorescenceinsituhybridizationanalysisofthehtercregioninacutemyeloidleukemiapatients AT zerrinyılmaz fluorescenceinsituhybridizationanalysisofthehtercregioninacutemyeloidleukemiapatients AT ferideiffetsahin fluorescenceinsituhybridizationanalysisofthehtercregioninacutemyeloidleukemiapatients |