Evaluation of the Effectiveness of Overheated Dry-Saturated Steam Disinfection in the Control of the Dental Chair Contamination by Bioluminescence Analysis: A Pilot In Vitro Study
This study aimed to evaluate, through Adenosine triphosphate (ATP) bioluminescence analysis, the effectiveness of an overheated dry-saturated steam device (<i>Polti Sani System</i>) in decreasing the superficial microbial contamination on dental chairs’ surfaces after 30 s steam disinfec...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/13/6/3685 |
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| author | Valentina Luppieri Manuela Giangreco Maddalena Chermetz Luca Ronfani Milena Cadenaro |
| author_facet | Valentina Luppieri Manuela Giangreco Maddalena Chermetz Luca Ronfani Milena Cadenaro |
| author_sort | Valentina Luppieri |
| collection | DOAJ |
| description | This study aimed to evaluate, through Adenosine triphosphate (ATP) bioluminescence analysis, the effectiveness of an overheated dry-saturated steam device (<i>Polti Sani System</i>) in decreasing the superficial microbial contamination on dental chairs’ surfaces after 30 s steam disinfection (T1) in comparison to baseline (T0), i.e., at the end of an aerosol-generating procedure (AGDP), and to investigate any differences in the tested surfaces’ contamination at T0 in relation to the surface’s type. Three dental chair surfaces (scialytic lamp, control button panel, spit bowl), sized 10 × 10 cm each, were swabbed and analyzed before and after steam application. The procedure was repeated 20 times for a total of 60 before–after evaluations. Non-parametric tests were used to analyze Relative Light Unit (RLU) values and categorical data on the ATP molecules’ amount detected on the tested surfaces. Statistically significant differences were found for both RLU and categorical data for all surfaces, and each type of surface evaluated at T0 and T1 (<i>p</i> < 0.05). Differences in RLU among the tested surfaces at T0 were not significant. By reducing the microbial contamination on the evaluated surfaces, the overheated dry-saturated steam system was an effective measure for the disinfection of the dental chair’s surfaces after AGDPs, potentially reducing the risk of cross-infections. |
| first_indexed | 2024-03-11T06:58:55Z |
| format | Article |
| id | doaj.art-c62b92c403954639916ec538be533fa9 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-11T06:58:55Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-c62b92c403954639916ec538be533fa92023-11-17T09:25:20ZengMDPI AGApplied Sciences2076-34172023-03-01136368510.3390/app13063685Evaluation of the Effectiveness of Overheated Dry-Saturated Steam Disinfection in the Control of the Dental Chair Contamination by Bioluminescence Analysis: A Pilot In Vitro StudyValentina Luppieri0Manuela Giangreco1Maddalena Chermetz2Luca Ronfani3Milena Cadenaro4Institute for Maternal and Child Health–IRCCS “Burlo Garofolo”–Trieste, Via dell’Istria 65, 34137 Trieste, ItalyInstitute for Maternal and Child Health–IRCCS “Burlo Garofolo”–Trieste, Via dell’Istria 65, 34137 Trieste, ItalyInstitute for Maternal and Child Health–IRCCS “Burlo Garofolo”–Trieste, Via dell’Istria 65, 34137 Trieste, ItalyInstitute for Maternal and Child Health–IRCCS “Burlo Garofolo”–Trieste, Via dell’Istria 65, 34137 Trieste, ItalyInstitute for Maternal and Child Health–IRCCS “Burlo Garofolo”–Trieste, Via dell’Istria 65, 34137 Trieste, ItalyThis study aimed to evaluate, through Adenosine triphosphate (ATP) bioluminescence analysis, the effectiveness of an overheated dry-saturated steam device (<i>Polti Sani System</i>) in decreasing the superficial microbial contamination on dental chairs’ surfaces after 30 s steam disinfection (T1) in comparison to baseline (T0), i.e., at the end of an aerosol-generating procedure (AGDP), and to investigate any differences in the tested surfaces’ contamination at T0 in relation to the surface’s type. Three dental chair surfaces (scialytic lamp, control button panel, spit bowl), sized 10 × 10 cm each, were swabbed and analyzed before and after steam application. The procedure was repeated 20 times for a total of 60 before–after evaluations. Non-parametric tests were used to analyze Relative Light Unit (RLU) values and categorical data on the ATP molecules’ amount detected on the tested surfaces. Statistically significant differences were found for both RLU and categorical data for all surfaces, and each type of surface evaluated at T0 and T1 (<i>p</i> < 0.05). Differences in RLU among the tested surfaces at T0 were not significant. By reducing the microbial contamination on the evaluated surfaces, the overheated dry-saturated steam system was an effective measure for the disinfection of the dental chair’s surfaces after AGDPs, potentially reducing the risk of cross-infections.https://www.mdpi.com/2076-3417/13/6/3685microbial contaminationaerosol-generating dental proceduresinfection controloverheated dry-saturated steam disinfectiondental chair |
| spellingShingle | Valentina Luppieri Manuela Giangreco Maddalena Chermetz Luca Ronfani Milena Cadenaro Evaluation of the Effectiveness of Overheated Dry-Saturated Steam Disinfection in the Control of the Dental Chair Contamination by Bioluminescence Analysis: A Pilot In Vitro Study Applied Sciences microbial contamination aerosol-generating dental procedures infection control overheated dry-saturated steam disinfection dental chair |
| title | Evaluation of the Effectiveness of Overheated Dry-Saturated Steam Disinfection in the Control of the Dental Chair Contamination by Bioluminescence Analysis: A Pilot In Vitro Study |
| title_full | Evaluation of the Effectiveness of Overheated Dry-Saturated Steam Disinfection in the Control of the Dental Chair Contamination by Bioluminescence Analysis: A Pilot In Vitro Study |
| title_fullStr | Evaluation of the Effectiveness of Overheated Dry-Saturated Steam Disinfection in the Control of the Dental Chair Contamination by Bioluminescence Analysis: A Pilot In Vitro Study |
| title_full_unstemmed | Evaluation of the Effectiveness of Overheated Dry-Saturated Steam Disinfection in the Control of the Dental Chair Contamination by Bioluminescence Analysis: A Pilot In Vitro Study |
| title_short | Evaluation of the Effectiveness of Overheated Dry-Saturated Steam Disinfection in the Control of the Dental Chair Contamination by Bioluminescence Analysis: A Pilot In Vitro Study |
| title_sort | evaluation of the effectiveness of overheated dry saturated steam disinfection in the control of the dental chair contamination by bioluminescence analysis a pilot in vitro study |
| topic | microbial contamination aerosol-generating dental procedures infection control overheated dry-saturated steam disinfection dental chair |
| url | https://www.mdpi.com/2076-3417/13/6/3685 |
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