Characteristics of HIV-1 natural drug resistance-associated mutations in former paid blood donors in Henan Province, China.
BACKGROUND: Natural drug resistance is a major cause of antiviral treatment failure. The characteristics of HIV-1 natural drug resistance-associated mutations in former paid blood donors in Henan Province remain unclear. METHODS: One hundred and fifty HIV-1-positive plasma samples were collected. Pl...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3929713?pdf=render |
| _version_ | 1811274054956482560 |
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| author | Yi Bao Di Tian Ying-Ying Zheng Hong-Li Xi Dan Liu Min Yu Xiao-Yuan Xu |
| author_facet | Yi Bao Di Tian Ying-Ying Zheng Hong-Li Xi Dan Liu Min Yu Xiao-Yuan Xu |
| author_sort | Yi Bao |
| collection | DOAJ |
| description | BACKGROUND: Natural drug resistance is a major cause of antiviral treatment failure. The characteristics of HIV-1 natural drug resistance-associated mutations in former paid blood donors in Henan Province remain unclear. METHODS: One hundred and fifty HIV-1-positive plasma samples were collected. Plasma viral RNA was extracted for pol gene amplification and sequencing. The sequencing results were submitted to the HIV-1 drug resistance database for drug-resistance analysis. RESULTS: The rates of natural drug resistance and resistance-associated mutations were 17.7% (19/107) and 40.2% (43/107), respectively. The rates of PI major, PI minor, NRTI, and NNRTI mutations were: 0, 30.8% (33/107), 10.3% (11/107), and 18.7% (20/107), respectively. Nine cases (8.4%) had both NRTI and NNRTI resistance-associated mutations. Seven cases (6.5%) had PI minor, NRTI and NNRTI resistance-associated mutations. NNRTI resistance was the most serious, followed by NRTI resistance and PI resistance. Polymorphism mutation sites with mutation rates in the protease region higher than 60.0% were: L63A/P/S/T 89.7%, V77I 82.2%, I72E/M/K/T/V 80.4%, I93L 75.7%, and E35D 72.9%. Polymorphism mutation sites with mutation rates in the RT region higher than 60.0% were: I135A/L/M/R/T/V 93.5%, T200A/E/I/P/V 89.7%, Q278E/K/N/T 88.8%, S162C/Y 82.2%, and K277R/S 66.4%. The distribution of 107 gene sequences was scattered, with some drug-resistant strains grouped in the same cluster. CONCLUSION: The natural drug resistance mutation rate of HIV-1 in former paid blood donors in Henan Province was 17.7%, with NNRTI resistance the most serious. The distribution of drug-resistant strains was scattered, with some correlations found in certain resistance loci. |
| first_indexed | 2024-04-12T23:10:58Z |
| format | Article |
| id | doaj.art-c62be166771644a3a9c04f3e02feda93 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T23:10:58Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c62be166771644a3a9c04f3e02feda932022-12-22T03:12:48ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e8929110.1371/journal.pone.0089291Characteristics of HIV-1 natural drug resistance-associated mutations in former paid blood donors in Henan Province, China.Yi BaoDi TianYing-Ying ZhengHong-Li XiDan LiuMin YuXiao-Yuan XuBACKGROUND: Natural drug resistance is a major cause of antiviral treatment failure. The characteristics of HIV-1 natural drug resistance-associated mutations in former paid blood donors in Henan Province remain unclear. METHODS: One hundred and fifty HIV-1-positive plasma samples were collected. Plasma viral RNA was extracted for pol gene amplification and sequencing. The sequencing results were submitted to the HIV-1 drug resistance database for drug-resistance analysis. RESULTS: The rates of natural drug resistance and resistance-associated mutations were 17.7% (19/107) and 40.2% (43/107), respectively. The rates of PI major, PI minor, NRTI, and NNRTI mutations were: 0, 30.8% (33/107), 10.3% (11/107), and 18.7% (20/107), respectively. Nine cases (8.4%) had both NRTI and NNRTI resistance-associated mutations. Seven cases (6.5%) had PI minor, NRTI and NNRTI resistance-associated mutations. NNRTI resistance was the most serious, followed by NRTI resistance and PI resistance. Polymorphism mutation sites with mutation rates in the protease region higher than 60.0% were: L63A/P/S/T 89.7%, V77I 82.2%, I72E/M/K/T/V 80.4%, I93L 75.7%, and E35D 72.9%. Polymorphism mutation sites with mutation rates in the RT region higher than 60.0% were: I135A/L/M/R/T/V 93.5%, T200A/E/I/P/V 89.7%, Q278E/K/N/T 88.8%, S162C/Y 82.2%, and K277R/S 66.4%. The distribution of 107 gene sequences was scattered, with some drug-resistant strains grouped in the same cluster. CONCLUSION: The natural drug resistance mutation rate of HIV-1 in former paid blood donors in Henan Province was 17.7%, with NNRTI resistance the most serious. The distribution of drug-resistant strains was scattered, with some correlations found in certain resistance loci.http://europepmc.org/articles/PMC3929713?pdf=render |
| spellingShingle | Yi Bao Di Tian Ying-Ying Zheng Hong-Li Xi Dan Liu Min Yu Xiao-Yuan Xu Characteristics of HIV-1 natural drug resistance-associated mutations in former paid blood donors in Henan Province, China. PLoS ONE |
| title | Characteristics of HIV-1 natural drug resistance-associated mutations in former paid blood donors in Henan Province, China. |
| title_full | Characteristics of HIV-1 natural drug resistance-associated mutations in former paid blood donors in Henan Province, China. |
| title_fullStr | Characteristics of HIV-1 natural drug resistance-associated mutations in former paid blood donors in Henan Province, China. |
| title_full_unstemmed | Characteristics of HIV-1 natural drug resistance-associated mutations in former paid blood donors in Henan Province, China. |
| title_short | Characteristics of HIV-1 natural drug resistance-associated mutations in former paid blood donors in Henan Province, China. |
| title_sort | characteristics of hiv 1 natural drug resistance associated mutations in former paid blood donors in henan province china |
| url | http://europepmc.org/articles/PMC3929713?pdf=render |
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