MRI of Transgene Expression: Correlation to Therapeutic Gene Expression
Magnetic resonance imaging (MRI) can provide highresolution 3D maps of structural and functional information, yet its use of mapping in vivo gene expression has only recently been explored. A potential application for this technology is to noninvasively image transgene expression. The current study...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2002-01-01
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| Series: | Neoplasia: An International Journal for Oncology Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1476558602800574 |
| _version_ | 1818564862600544256 |
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| author | Tomotsugu Ichikawa Dagmar Högemann Yoshinaga Saeki Edyta Tyminski Kinya Terada Ralph Weissleder E. Antonio Chiocca James P. Basilion |
| author_facet | Tomotsugu Ichikawa Dagmar Högemann Yoshinaga Saeki Edyta Tyminski Kinya Terada Ralph Weissleder E. Antonio Chiocca James P. Basilion |
| author_sort | Tomotsugu Ichikawa |
| collection | DOAJ |
| description | Magnetic resonance imaging (MRI) can provide highresolution 3D maps of structural and functional information, yet its use of mapping in vivo gene expression has only recently been explored. A potential application for this technology is to noninvasively image transgene expression. The current study explores the latter using a nonregulatable internalizing engineered transferrin receptor (ETR) whose expression can be probed for with a superparamagnetic Tf-CLIO probe. Using an HSV-based amplicon vector system for transgene delivery, we demonstrate that: 1) ETR is a sensitive MR marker gene; 2) several transgenes can be efficiently expressed from a single amplicon; 3) expression of each transgene results in functional gene product; and 4) ETR gene expression correlates with expression of therapeutic genes when the latter are contained within the same amplicon. These data, taken together, suggest that MRI of ETR expression can serve as a surrogate for measuring therapeutic transgene expression. |
| first_indexed | 2024-12-14T01:34:04Z |
| format | Article |
| id | doaj.art-c62c2c31b86b4f0b85a5e26f2452e144 |
| institution | Directory Open Access Journal |
| issn | 1476-5586 1522-8002 |
| language | English |
| last_indexed | 2024-12-14T01:34:04Z |
| publishDate | 2002-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neoplasia: An International Journal for Oncology Research |
| spelling | doaj.art-c62c2c31b86b4f0b85a5e26f2452e1442022-12-21T23:21:57ZengElsevierNeoplasia: An International Journal for Oncology Research1476-55861522-80022002-01-014652353010.1038/sj.neo.7900266MRI of Transgene Expression: Correlation to Therapeutic Gene ExpressionTomotsugu Ichikawa0Dagmar Högemann1Yoshinaga Saeki2Edyta Tyminski3Kinya Terada4Ralph Weissleder5E. Antonio Chiocca6James P. Basilion7Neurosurgical Service and Molecular Neuro-Oncology Laboratory, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USACenter for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USANeurosurgical Service and Molecular Neuro-Oncology Laboratory, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USANeurosurgical Service and Molecular Neuro-Oncology Laboratory, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USANeurosurgical Service and Molecular Neuro-Oncology Laboratory, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USACenter for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USANeurosurgical Service and Molecular Neuro-Oncology Laboratory, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USANeurosurgical Service and Molecular Neuro-Oncology Laboratory, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USAMagnetic resonance imaging (MRI) can provide highresolution 3D maps of structural and functional information, yet its use of mapping in vivo gene expression has only recently been explored. A potential application for this technology is to noninvasively image transgene expression. The current study explores the latter using a nonregulatable internalizing engineered transferrin receptor (ETR) whose expression can be probed for with a superparamagnetic Tf-CLIO probe. Using an HSV-based amplicon vector system for transgene delivery, we demonstrate that: 1) ETR is a sensitive MR marker gene; 2) several transgenes can be efficiently expressed from a single amplicon; 3) expression of each transgene results in functional gene product; and 4) ETR gene expression correlates with expression of therapeutic genes when the latter are contained within the same amplicon. These data, taken together, suggest that MRI of ETR expression can serve as a surrogate for measuring therapeutic transgene expression.http://www.sciencedirect.com/science/article/pii/S1476558602800574CLIOETRtransgeneentailedMRI |
| spellingShingle | Tomotsugu Ichikawa Dagmar Högemann Yoshinaga Saeki Edyta Tyminski Kinya Terada Ralph Weissleder E. Antonio Chiocca James P. Basilion MRI of Transgene Expression: Correlation to Therapeutic Gene Expression Neoplasia: An International Journal for Oncology Research CLIO ETR transgene entailed MRI |
| title | MRI of Transgene Expression: Correlation to Therapeutic Gene Expression |
| title_full | MRI of Transgene Expression: Correlation to Therapeutic Gene Expression |
| title_fullStr | MRI of Transgene Expression: Correlation to Therapeutic Gene Expression |
| title_full_unstemmed | MRI of Transgene Expression: Correlation to Therapeutic Gene Expression |
| title_short | MRI of Transgene Expression: Correlation to Therapeutic Gene Expression |
| title_sort | mri of transgene expression correlation to therapeutic gene expression |
| topic | CLIO ETR transgene entailed MRI |
| url | http://www.sciencedirect.com/science/article/pii/S1476558602800574 |
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