MicroRNA-223-3p downregulates the inflammatory response in preeclampsia placenta via targeting NLRP3
Abstract Objective To investigate the regulatory role of miR-223-3p in the inflammatory response of PE placenta. Methods PE and normal placental tissues were collected to measure the expression of NLRP3 and miR-223-3p. The targeting relationship between NLRP3 and miR-223-3P was verified by bioinform...
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | BMC Pregnancy and Childbirth |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12884-024-06371-9 |
| _version_ | 1797266590285168640 |
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| author | Xueqiong Liu Zhiyue Li Dan Lu |
| author_facet | Xueqiong Liu Zhiyue Li Dan Lu |
| author_sort | Xueqiong Liu |
| collection | DOAJ |
| description | Abstract Objective To investigate the regulatory role of miR-223-3p in the inflammatory response of PE placenta. Methods PE and normal placental tissues were collected to measure the expression of NLRP3 and miR-223-3p. The targeting relationship between NLRP3 and miR-223-3P was verified by bioinformatics analysis and classical double-luciferase reporter gene assay. Lipopolysaccharide (LPS) was used to induce HTR8/SVneo cells as PE placental cell inflammation model. Then we transfected miR-223-3p overexpression/miR-223-3p negative control plasmid into the LPS-induced HTR8/SVneo cells. Next, the expressions of NLRP3, Caspase-1, GSDMD, IL-1β and IL-18 were evaluated to elucidate the regulatory effect of miR-223-3p on the inflammatory response mediated by NLRP3 in PE placenta. Results Compared with normal controls, NLRP3 was significantly up-regulated in PE placenta, while miR-223-3p was down-regulated. In addition, NLRP3 was a direct target of miR-223-3p. Further research revealed that the expression of NLRP3, Caspase-1, GSDMD, IL-1β and IL-18 could be obviously promoted in HTR8/SVneo cells treated with LPS (500 ng/ml) for 24 h, nevertheless it could be significantly suppressesed under the overexpression of miR-223-3p. Conclusion MiR-223-3p suppressed NLRP3 inflamariomes activation, downstream inflammatory factors secretion and pyroptosis in LPS-induced HTR8/SVneo cells indicating that miR-223-3p could serve as an anti-inflammatory factor in preeclampsia. |
| first_indexed | 2024-04-25T01:03:06Z |
| format | Article |
| id | doaj.art-c62e4ab56ece407da0394111efa7bc17 |
| institution | Directory Open Access Journal |
| issn | 1471-2393 |
| language | English |
| last_indexed | 2024-04-25T01:03:06Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pregnancy and Childbirth |
| spelling | doaj.art-c62e4ab56ece407da0394111efa7bc172024-03-10T12:24:07ZengBMCBMC Pregnancy and Childbirth1471-23932024-03-0124111210.1186/s12884-024-06371-9MicroRNA-223-3p downregulates the inflammatory response in preeclampsia placenta via targeting NLRP3Xueqiong Liu0Zhiyue Li1Dan Lu2Clinical Medical College of Yangzhou UniversityClinical Medical College of Yangzhou UniversityClinical Medical College of Yangzhou UniversityAbstract Objective To investigate the regulatory role of miR-223-3p in the inflammatory response of PE placenta. Methods PE and normal placental tissues were collected to measure the expression of NLRP3 and miR-223-3p. The targeting relationship between NLRP3 and miR-223-3P was verified by bioinformatics analysis and classical double-luciferase reporter gene assay. Lipopolysaccharide (LPS) was used to induce HTR8/SVneo cells as PE placental cell inflammation model. Then we transfected miR-223-3p overexpression/miR-223-3p negative control plasmid into the LPS-induced HTR8/SVneo cells. Next, the expressions of NLRP3, Caspase-1, GSDMD, IL-1β and IL-18 were evaluated to elucidate the regulatory effect of miR-223-3p on the inflammatory response mediated by NLRP3 in PE placenta. Results Compared with normal controls, NLRP3 was significantly up-regulated in PE placenta, while miR-223-3p was down-regulated. In addition, NLRP3 was a direct target of miR-223-3p. Further research revealed that the expression of NLRP3, Caspase-1, GSDMD, IL-1β and IL-18 could be obviously promoted in HTR8/SVneo cells treated with LPS (500 ng/ml) for 24 h, nevertheless it could be significantly suppressesed under the overexpression of miR-223-3p. Conclusion MiR-223-3p suppressed NLRP3 inflamariomes activation, downstream inflammatory factors secretion and pyroptosis in LPS-induced HTR8/SVneo cells indicating that miR-223-3p could serve as an anti-inflammatory factor in preeclampsia.https://doi.org/10.1186/s12884-024-06371-9PreeclampsiaPlacenta inflammatory dysregulationNLRP3 inflammasomemicroRNA-223-3p |
| spellingShingle | Xueqiong Liu Zhiyue Li Dan Lu MicroRNA-223-3p downregulates the inflammatory response in preeclampsia placenta via targeting NLRP3 BMC Pregnancy and Childbirth Preeclampsia Placenta inflammatory dysregulation NLRP3 inflammasome microRNA-223-3p |
| title | MicroRNA-223-3p downregulates the inflammatory response in preeclampsia placenta via targeting NLRP3 |
| title_full | MicroRNA-223-3p downregulates the inflammatory response in preeclampsia placenta via targeting NLRP3 |
| title_fullStr | MicroRNA-223-3p downregulates the inflammatory response in preeclampsia placenta via targeting NLRP3 |
| title_full_unstemmed | MicroRNA-223-3p downregulates the inflammatory response in preeclampsia placenta via targeting NLRP3 |
| title_short | MicroRNA-223-3p downregulates the inflammatory response in preeclampsia placenta via targeting NLRP3 |
| title_sort | microrna 223 3p downregulates the inflammatory response in preeclampsia placenta via targeting nlrp3 |
| topic | Preeclampsia Placenta inflammatory dysregulation NLRP3 inflammasome microRNA-223-3p |
| url | https://doi.org/10.1186/s12884-024-06371-9 |
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