Translation regulatory long non-coding RNA 1 (TRERNA1) sponges microRNA-23a to suppress granulosa cell apoptosis in premature ovarian failure
Translation regulatory long non-coding RNA 1 (TRERNA1) plays critical roles in cancer biology. We predicted the direct interaction of TRERNA1 with microRNA (miR)-23a, which promotes granulosa apoptosis. Granulosa apoptosis is involved in premature ovarian failure (POF). This study was therefore carr...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-02-01
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| Series: | Bioengineered |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21655979.2021.2023802 |
| _version_ | 1798014867546832896 |
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| author | Lili Zhang Bin Mao Xiaodong Zhao Yue Yuan Wei Wang Shaohua Lin |
| author_facet | Lili Zhang Bin Mao Xiaodong Zhao Yue Yuan Wei Wang Shaohua Lin |
| author_sort | Lili Zhang |
| collection | DOAJ |
| description | Translation regulatory long non-coding RNA 1 (TRERNA1) plays critical roles in cancer biology. We predicted the direct interaction of TRERNA1 with microRNA (miR)-23a, which promotes granulosa apoptosis. Granulosa apoptosis is involved in premature ovarian failure (POF). This study was therefore carried out to explore the involvement of TRERNA1 and miR-23a in POF. The expression of TRERNA1 and miR-23a in POF and control groups were detected by RT-qPCRs. The subcellular locations of TRERNA1 in granulosa cell line COV434 was detected by subcellular fractionation assay. The interaction between TRERNA1 and miR-23a was predicted using IntaRNA2.0. The direct interaction between COV434 and miR-23a was explored with RNA pull-down assay. In granulosa cells, the direct interaction between TRERNA1 and miR-23a was verified by overexpression assay. Cell apoptosis assay was performed to evaluate cell apoptosis. Both TRERNA1 and miR-23a were downregulated in POF. In addition, TRERNA1 was detected in both cytoplasm and nuclear samples of granulosa cells, and directly interacted with miR-23a. TRERNA1 did not affect the expression of miR-23a in granulosa cells, while TRERNA1 suppressed the role of miR-23a in enhancing cell apoptosis. In conclusion, TRERNA1 may sponge miR-23a to suppress granulosa cell apoptosis in POF. |
| first_indexed | 2024-04-11T15:24:01Z |
| format | Article |
| id | doaj.art-c63284073c1843e187071be71fe5df7b |
| institution | Directory Open Access Journal |
| issn | 2165-5979 2165-5987 |
| language | English |
| last_indexed | 2024-04-11T15:24:01Z |
| publishDate | 2022-02-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Bioengineered |
| spelling | doaj.art-c63284073c1843e187071be71fe5df7b2022-12-22T04:16:18ZengTaylor & Francis GroupBioengineered2165-59792165-59872022-02-011322173218010.1080/21655979.2021.20238022023802Translation regulatory long non-coding RNA 1 (TRERNA1) sponges microRNA-23a to suppress granulosa cell apoptosis in premature ovarian failureLili Zhang0Bin Mao1Xiaodong Zhao2Yue Yuan3Wei Wang4Shaohua Lin5The Reproductive Medicine Center of the First Hospital of Lanzhou UniversityThe Reproductive Medicine Center of the First Hospital of Lanzhou UniversityThe Reproductive Medicine Center of the First Hospital of Lanzhou UniversityThe Reproductive Medicine Center of the First Hospital of Lanzhou UniversityThe Reproductive Medicine Center of the First Hospital of Lanzhou UniversityReproductive Department of Guangxi International Zhuang Medical HospitalTranslation regulatory long non-coding RNA 1 (TRERNA1) plays critical roles in cancer biology. We predicted the direct interaction of TRERNA1 with microRNA (miR)-23a, which promotes granulosa apoptosis. Granulosa apoptosis is involved in premature ovarian failure (POF). This study was therefore carried out to explore the involvement of TRERNA1 and miR-23a in POF. The expression of TRERNA1 and miR-23a in POF and control groups were detected by RT-qPCRs. The subcellular locations of TRERNA1 in granulosa cell line COV434 was detected by subcellular fractionation assay. The interaction between TRERNA1 and miR-23a was predicted using IntaRNA2.0. The direct interaction between COV434 and miR-23a was explored with RNA pull-down assay. In granulosa cells, the direct interaction between TRERNA1 and miR-23a was verified by overexpression assay. Cell apoptosis assay was performed to evaluate cell apoptosis. Both TRERNA1 and miR-23a were downregulated in POF. In addition, TRERNA1 was detected in both cytoplasm and nuclear samples of granulosa cells, and directly interacted with miR-23a. TRERNA1 did not affect the expression of miR-23a in granulosa cells, while TRERNA1 suppressed the role of miR-23a in enhancing cell apoptosis. In conclusion, TRERNA1 may sponge miR-23a to suppress granulosa cell apoptosis in POF.http://dx.doi.org/10.1080/21655979.2021.2023802trerna1mir-23apremature ovarian failureapoptosis |
| spellingShingle | Lili Zhang Bin Mao Xiaodong Zhao Yue Yuan Wei Wang Shaohua Lin Translation regulatory long non-coding RNA 1 (TRERNA1) sponges microRNA-23a to suppress granulosa cell apoptosis in premature ovarian failure Bioengineered trerna1 mir-23a premature ovarian failure apoptosis |
| title | Translation regulatory long non-coding RNA 1 (TRERNA1) sponges microRNA-23a to suppress granulosa cell apoptosis in premature ovarian failure |
| title_full | Translation regulatory long non-coding RNA 1 (TRERNA1) sponges microRNA-23a to suppress granulosa cell apoptosis in premature ovarian failure |
| title_fullStr | Translation regulatory long non-coding RNA 1 (TRERNA1) sponges microRNA-23a to suppress granulosa cell apoptosis in premature ovarian failure |
| title_full_unstemmed | Translation regulatory long non-coding RNA 1 (TRERNA1) sponges microRNA-23a to suppress granulosa cell apoptosis in premature ovarian failure |
| title_short | Translation regulatory long non-coding RNA 1 (TRERNA1) sponges microRNA-23a to suppress granulosa cell apoptosis in premature ovarian failure |
| title_sort | translation regulatory long non coding rna 1 trerna1 sponges microrna 23a to suppress granulosa cell apoptosis in premature ovarian failure |
| topic | trerna1 mir-23a premature ovarian failure apoptosis |
| url | http://dx.doi.org/10.1080/21655979.2021.2023802 |
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