miR-708–3p promotes gastric cancer progression through downregulating ETNK1
MicroRNAs (miRNAs) are small, evolutionarily conserved, non-coding RNAs playing a role in the proliferation, metastasis, apoptosis, chemo-sensitivity, and chemo-resistance of gastric cancer, as well as the stemness of gastric cancer stem cells. miR-708–3p induces gastric cancer cell chemo-resistance...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584402306752X |
| _version_ | 1797669862004228096 |
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| author | Jincai Shang Qingdong Wang Jingren Wang Bo Xu Shuang Liu |
| author_facet | Jincai Shang Qingdong Wang Jingren Wang Bo Xu Shuang Liu |
| author_sort | Jincai Shang |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are small, evolutionarily conserved, non-coding RNAs playing a role in the proliferation, metastasis, apoptosis, chemo-sensitivity, and chemo-resistance of gastric cancer, as well as the stemness of gastric cancer stem cells. miR-708–3p induces gastric cancer cell chemo-resistance, but its actual role in gastric cancer progression remains unclear. This paper shows that miR-708–3p is upregulated in gastric cancer samples and that a high miR-708–3p expression in gastric cancer patients is associated with poor overall survival. Our functional study results indicate that miR-708–3p overexpression promotes gastric cancer cell proliferation and migration, inhibits cell apoptosis, and facilitates the transition from the G0/G1 to the G2/M phase. Furthermore, reducing miR-708–3p levels yielded opposite effects. Next, our in vivo experiments revealed that miR-708–3p advanced gastric cancer cell growth in nude mice. The underlying mechanism was the regulation of ethanolamine kinase 1 (ETNK1) expression by miR-708–3p, which bound to the 3′UTR of the ETNK1 gene in gastric cancer cells. Finally, the recovery assay results showed that ETNK1 overexpression could slow miR-708-3p-induced gastric cancer progression. In conclusion, we identified a new miR-708–3p/ETNK1 pathway involved in gastric cancer progression. These results may offer new targets for gastric cancer therapy and markers for gastric cancer prognosis. |
| first_indexed | 2024-03-11T20:50:57Z |
| format | Article |
| id | doaj.art-c633a4a8ddef4d17a5e2888b89fa8e4d |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-11T20:50:57Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-c633a4a8ddef4d17a5e2888b89fa8e4d2023-10-01T06:00:02ZengElsevierHeliyon2405-84402023-09-0199e19544miR-708–3p promotes gastric cancer progression through downregulating ETNK1Jincai Shang0Qingdong Wang1Jingren Wang2Bo Xu3Shuang Liu4Key Laboratory of Microecology-immune Regulatory Network and Related Diseases, School of Basic Medicine, Jiamusi University, Jiamusi, Heilongjiang, 154000, ChinaKey Laboratory of Microecology-immune Regulatory Network and Related Diseases, School of Basic Medicine, Jiamusi University, Jiamusi, Heilongjiang, 154000, ChinaKey Laboratory of Microecology-immune Regulatory Network and Related Diseases, School of Basic Medicine, Jiamusi University, Jiamusi, Heilongjiang, 154000, ChinaKey Laboratory of Microecology-immune Regulatory Network and Related Diseases, School of Basic Medicine, Jiamusi University, Jiamusi, Heilongjiang, 154000, ChinaCorresponding author. Key Laboratory of Microecology-immune Regulatory Network and Related Diseases, School of Basic Medicine, Jiamusi University, No. 258, Xuefu Street, Jiamusi City, Heilongjiang Province, 154000, China.; Key Laboratory of Microecology-immune Regulatory Network and Related Diseases, School of Basic Medicine, Jiamusi University, Jiamusi, Heilongjiang, 154000, ChinaMicroRNAs (miRNAs) are small, evolutionarily conserved, non-coding RNAs playing a role in the proliferation, metastasis, apoptosis, chemo-sensitivity, and chemo-resistance of gastric cancer, as well as the stemness of gastric cancer stem cells. miR-708–3p induces gastric cancer cell chemo-resistance, but its actual role in gastric cancer progression remains unclear. This paper shows that miR-708–3p is upregulated in gastric cancer samples and that a high miR-708–3p expression in gastric cancer patients is associated with poor overall survival. Our functional study results indicate that miR-708–3p overexpression promotes gastric cancer cell proliferation and migration, inhibits cell apoptosis, and facilitates the transition from the G0/G1 to the G2/M phase. Furthermore, reducing miR-708–3p levels yielded opposite effects. Next, our in vivo experiments revealed that miR-708–3p advanced gastric cancer cell growth in nude mice. The underlying mechanism was the regulation of ethanolamine kinase 1 (ETNK1) expression by miR-708–3p, which bound to the 3′UTR of the ETNK1 gene in gastric cancer cells. Finally, the recovery assay results showed that ETNK1 overexpression could slow miR-708-3p-induced gastric cancer progression. In conclusion, we identified a new miR-708–3p/ETNK1 pathway involved in gastric cancer progression. These results may offer new targets for gastric cancer therapy and markers for gastric cancer prognosis.http://www.sciencedirect.com/science/article/pii/S240584402306752XmiR-708–3pETNK1Gastric cancer |
| spellingShingle | Jincai Shang Qingdong Wang Jingren Wang Bo Xu Shuang Liu miR-708–3p promotes gastric cancer progression through downregulating ETNK1 Heliyon miR-708–3p ETNK1 Gastric cancer |
| title | miR-708–3p promotes gastric cancer progression through downregulating ETNK1 |
| title_full | miR-708–3p promotes gastric cancer progression through downregulating ETNK1 |
| title_fullStr | miR-708–3p promotes gastric cancer progression through downregulating ETNK1 |
| title_full_unstemmed | miR-708–3p promotes gastric cancer progression through downregulating ETNK1 |
| title_short | miR-708–3p promotes gastric cancer progression through downregulating ETNK1 |
| title_sort | mir 708 3p promotes gastric cancer progression through downregulating etnk1 |
| topic | miR-708–3p ETNK1 Gastric cancer |
| url | http://www.sciencedirect.com/science/article/pii/S240584402306752X |
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