Effect of Heat-Killed <i>Lactobacillus casei</i> DKGF7 on a Rat Model of Irritable Bowel Syndrome
Non-viable bacteria, referred to as “paraprobiotics,” have attracted attention as potentially safer alternatives to probiotics. The aim of this study was to investigate the efficacy of heat-killed <i>Lactobacillus casei</i> DKGF7 on the symptomatic improvement of irritable bowel syndrome...
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MDPI AG
2021-02-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/13/2/568 |
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| author | Gyeol Seong Seungbaek Lee Yang Won Min Yeon Sil Jang Hong Seog Kim Eui-Joong Kim So-Young Park Cheol-Hyun Kim Dong Kyung Chang |
| author_facet | Gyeol Seong Seungbaek Lee Yang Won Min Yeon Sil Jang Hong Seog Kim Eui-Joong Kim So-Young Park Cheol-Hyun Kim Dong Kyung Chang |
| author_sort | Gyeol Seong |
| collection | DOAJ |
| description | Non-viable bacteria, referred to as “paraprobiotics,” have attracted attention as potentially safer alternatives to probiotics. The aim of this study was to investigate the efficacy of heat-killed <i>Lactobacillus casei</i> DKGF7 on the symptomatic improvement of irritable bowel syndrome (IBS) in a rat disease model and to elucidate the underlying mechanisms that contribute to the beneficial effects of heat-killed probiotics. Seven male Wistar rats were induced with IBS by restraint stress and administered heat-killed <i>L. casei</i> DKGF7 for four weeks and then compared with seven rats in the control group. Stool consistency measured four weeks after initial treatment was the primary outcome measure. To investigate the mechanism of action of the heat-killed bacteria on IBS, we measured serum corticosterone levels, inflammatory cytokines in colon tissue, and expression of tight junction proteins (TJPs) in the epithelium. The treatment group showed significantly better stool consistency scores than the control group at week 4, as well as at every measured time point (all <i>p</i> values < 0.05). The treatment group showed lower serum corticosterone levels, lower colonic inflammatory cytokine levels, and higher expression of TJPs compared with the control group. Paraprobiotics such as heat-killed <i>L. casei</i> DKGF7 can improve stool consistency in a rat IBS model, which may indicate a potential therapeutic strategy for IBS treatment. |
| first_indexed | 2024-03-09T05:00:27Z |
| format | Article |
| id | doaj.art-c6359960270d4e8d87c9362cdcc2d7ab |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T05:00:27Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-c6359960270d4e8d87c9362cdcc2d7ab2023-12-03T13:00:27ZengMDPI AGNutrients2072-66432021-02-0113256810.3390/nu13020568Effect of Heat-Killed <i>Lactobacillus casei</i> DKGF7 on a Rat Model of Irritable Bowel SyndromeGyeol Seong0Seungbaek Lee1Yang Won Min2Yeon Sil Jang3Hong Seog Kim4Eui-Joong Kim5So-Young Park6Cheol-Hyun Kim7Dong Kyung Chang8Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, KoreaDepartment of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, KoreaDepartment of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, KoreaDepartment of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, KoreaYoudam Co., Ltd. 65, Namsan 2-gil, Jiksan-eup, Seobuk-gu, Cheonan-si 31116, Chungcheongnam-do, KoreaGenofocus, Inc., 65, Techno 1-ro, Yuseong-gu, Daejeon 34014, KoreaLaboratory of Pharmacognosy, College of Pharmacy, Dankook University, Cheonan 31116, KoreaCollege of Biotechnology & Bioengineering, Dankook University, Cheonan 31116, KoreaDepartment of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, KoreaNon-viable bacteria, referred to as “paraprobiotics,” have attracted attention as potentially safer alternatives to probiotics. The aim of this study was to investigate the efficacy of heat-killed <i>Lactobacillus casei</i> DKGF7 on the symptomatic improvement of irritable bowel syndrome (IBS) in a rat disease model and to elucidate the underlying mechanisms that contribute to the beneficial effects of heat-killed probiotics. Seven male Wistar rats were induced with IBS by restraint stress and administered heat-killed <i>L. casei</i> DKGF7 for four weeks and then compared with seven rats in the control group. Stool consistency measured four weeks after initial treatment was the primary outcome measure. To investigate the mechanism of action of the heat-killed bacteria on IBS, we measured serum corticosterone levels, inflammatory cytokines in colon tissue, and expression of tight junction proteins (TJPs) in the epithelium. The treatment group showed significantly better stool consistency scores than the control group at week 4, as well as at every measured time point (all <i>p</i> values < 0.05). The treatment group showed lower serum corticosterone levels, lower colonic inflammatory cytokine levels, and higher expression of TJPs compared with the control group. Paraprobiotics such as heat-killed <i>L. casei</i> DKGF7 can improve stool consistency in a rat IBS model, which may indicate a potential therapeutic strategy for IBS treatment.https://www.mdpi.com/2072-6643/13/2/568irritable bowel syndrome<i>Lactobacillus casei</i>probioticscorticosteronecytokinestight junction proteins |
| spellingShingle | Gyeol Seong Seungbaek Lee Yang Won Min Yeon Sil Jang Hong Seog Kim Eui-Joong Kim So-Young Park Cheol-Hyun Kim Dong Kyung Chang Effect of Heat-Killed <i>Lactobacillus casei</i> DKGF7 on a Rat Model of Irritable Bowel Syndrome Nutrients irritable bowel syndrome <i>Lactobacillus casei</i> probiotics corticosterone cytokines tight junction proteins |
| title | Effect of Heat-Killed <i>Lactobacillus casei</i> DKGF7 on a Rat Model of Irritable Bowel Syndrome |
| title_full | Effect of Heat-Killed <i>Lactobacillus casei</i> DKGF7 on a Rat Model of Irritable Bowel Syndrome |
| title_fullStr | Effect of Heat-Killed <i>Lactobacillus casei</i> DKGF7 on a Rat Model of Irritable Bowel Syndrome |
| title_full_unstemmed | Effect of Heat-Killed <i>Lactobacillus casei</i> DKGF7 on a Rat Model of Irritable Bowel Syndrome |
| title_short | Effect of Heat-Killed <i>Lactobacillus casei</i> DKGF7 on a Rat Model of Irritable Bowel Syndrome |
| title_sort | effect of heat killed i lactobacillus casei i dkgf7 on a rat model of irritable bowel syndrome |
| topic | irritable bowel syndrome <i>Lactobacillus casei</i> probiotics corticosterone cytokines tight junction proteins |
| url | https://www.mdpi.com/2072-6643/13/2/568 |
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