Interlaboratory study of ethanol usage as an internal standard in direct determination of volatile compounds in alcoholic products
A collaborative interlaboratory study on the method of direct quantitation of volatile compounds in spirit drinks and alcoholic products was conducted. The discussed method applies ethanol, the major volatile component of an alcoholic product, as an internal standard. In this study 9 laboratories fr...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2019-01-01
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| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/full_html/2019/04/bioconf-oiv2019_02030/bioconf-oiv2019_02030.html |
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| author | Charapitsa S. Sytova S. Korban A. Sobolenko L. Egorov V. Leschev S. Zakharov M. Čabala R. Busarova R. Shestakovich I. Tolstouhova A. Ondroušek S. Vávra J. Yilmaztekin M. Cabaroglu T. |
| author_facet | Charapitsa S. Sytova S. Korban A. Sobolenko L. Egorov V. Leschev S. Zakharov M. Čabala R. Busarova R. Shestakovich I. Tolstouhova A. Ondroušek S. Vávra J. Yilmaztekin M. Cabaroglu T. |
| author_sort | Charapitsa S. |
| collection | DOAJ |
| description | A collaborative interlaboratory study on the method of direct quantitation of volatile compounds in spirit drinks and alcoholic products was conducted. The discussed method applies ethanol, the major volatile component of an alcoholic product, as an internal standard. In this study 9 laboratories from 4 different countries were supplied with standard solutions for gas chromatographic measurements. Five aqueous ethanol 40% (v/v) standard solutions containing target compounds in concentrations ranging from 10 mg/L to 400 mg/L of absolute alcohol were prepared and sent to the participants for quantification of acetaldehyde, methyl acetate, ethyl acetate, methanol, 2-propanol, 1-propanol, 2-methyl-1-propanol, 1-butanol and 3-methyl-1-butanol. The interlaboratory study was evaluated according to the ISO 5725 standards and the Eurachem guide. The within-laboratory precision varied between 0.4% and 7.5% for all samples and compounds, showing a sufficiently high repeatability of the method. The between-laboratory precision was found to vary within a satisfactory range of 0.5% ÷ 10.0%. Precision of the method was well within the range predicted by the Horwitz equation for all analytes. The analysis of trueness showed that the bias of the method is insignificant at the significance level α = 5%. The determined concentrations of the analytes compared well to the gravimetrical values thus showing very satisfactory accuracy of the method. The results of the interlaboratory study confirmed that “Ethanol as Internal Standard” method is robust and reliable and can be used as a standard reference method for analysing volatile compounds in water-ethanol samples. The possibilities of method validation according to the previously obtained experimental data were shown. |
| first_indexed | 2024-12-21T10:45:51Z |
| format | Article |
| id | doaj.art-c63b14589c2f4722ae1aa06847a1216d |
| institution | Directory Open Access Journal |
| issn | 2117-4458 |
| language | English |
| last_indexed | 2024-12-21T10:45:51Z |
| publishDate | 2019-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj.art-c63b14589c2f4722ae1aa06847a1216d2022-12-21T19:06:49ZengEDP SciencesBIO Web of Conferences2117-44582019-01-01150203010.1051/bioconf/20191502030bioconf-oiv2019_02030Interlaboratory study of ethanol usage as an internal standard in direct determination of volatile compounds in alcoholic productsCharapitsa S.0Sytova S.1Korban A.Sobolenko L.Egorov V.2Leschev S.3Zakharov M.4Čabala R.5Busarova R.6Shestakovich I.7Tolstouhova A.8Ondroušek S.9Vávra J.10Yilmaztekin M.11Cabaroglu T.12Institute for Nuclear Problems of Belarusian State UniversityInstitute for Nuclear Problems of Belarusian State UniversityChemistry Faculty, Department of Analytical Chemistry, Belarusian State UniversityChemistry Faculty, Department of Analytical Chemistry, Belarusian State UniversityAll-Russian Scientific Research Institute of the Brewing, Non-Alcoholic and Wine Industry – Branch of the V.M. Gorbatov Federal Scientific Center of Food Systems of RASCharles University, Faculty of Science, Department of Analytical ChemistryJS “Mosazervinzavod”Republican Centre for Hygiene, Epidemiology and Public HealthRepublican Centre for Hygiene, Epidemiology and Public HealthGeneral Directorate of Customs, Customs Technical LaboratoryGeneral Directorate of Customs, Customs Technical LaboratoryInonu University, Faculty of Engineering, Department of Food EngineeringCukurova University, Faculty of Agriculture, Department of Food EngineeringA collaborative interlaboratory study on the method of direct quantitation of volatile compounds in spirit drinks and alcoholic products was conducted. The discussed method applies ethanol, the major volatile component of an alcoholic product, as an internal standard. In this study 9 laboratories from 4 different countries were supplied with standard solutions for gas chromatographic measurements. Five aqueous ethanol 40% (v/v) standard solutions containing target compounds in concentrations ranging from 10 mg/L to 400 mg/L of absolute alcohol were prepared and sent to the participants for quantification of acetaldehyde, methyl acetate, ethyl acetate, methanol, 2-propanol, 1-propanol, 2-methyl-1-propanol, 1-butanol and 3-methyl-1-butanol. The interlaboratory study was evaluated according to the ISO 5725 standards and the Eurachem guide. The within-laboratory precision varied between 0.4% and 7.5% for all samples and compounds, showing a sufficiently high repeatability of the method. The between-laboratory precision was found to vary within a satisfactory range of 0.5% ÷ 10.0%. Precision of the method was well within the range predicted by the Horwitz equation for all analytes. The analysis of trueness showed that the bias of the method is insignificant at the significance level α = 5%. The determined concentrations of the analytes compared well to the gravimetrical values thus showing very satisfactory accuracy of the method. The results of the interlaboratory study confirmed that “Ethanol as Internal Standard” method is robust and reliable and can be used as a standard reference method for analysing volatile compounds in water-ethanol samples. The possibilities of method validation according to the previously obtained experimental data were shown.https://www.bio-conferences.org/articles/bioconf/full_html/2019/04/bioconf-oiv2019_02030/bioconf-oiv2019_02030.html |
| spellingShingle | Charapitsa S. Sytova S. Korban A. Sobolenko L. Egorov V. Leschev S. Zakharov M. Čabala R. Busarova R. Shestakovich I. Tolstouhova A. Ondroušek S. Vávra J. Yilmaztekin M. Cabaroglu T. Interlaboratory study of ethanol usage as an internal standard in direct determination of volatile compounds in alcoholic products BIO Web of Conferences |
| title | Interlaboratory study of ethanol usage as an internal standard in direct determination of volatile compounds in alcoholic products |
| title_full | Interlaboratory study of ethanol usage as an internal standard in direct determination of volatile compounds in alcoholic products |
| title_fullStr | Interlaboratory study of ethanol usage as an internal standard in direct determination of volatile compounds in alcoholic products |
| title_full_unstemmed | Interlaboratory study of ethanol usage as an internal standard in direct determination of volatile compounds in alcoholic products |
| title_short | Interlaboratory study of ethanol usage as an internal standard in direct determination of volatile compounds in alcoholic products |
| title_sort | interlaboratory study of ethanol usage as an internal standard in direct determination of volatile compounds in alcoholic products |
| url | https://www.bio-conferences.org/articles/bioconf/full_html/2019/04/bioconf-oiv2019_02030/bioconf-oiv2019_02030.html |
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