Somatostatin-Mediated Regulation of Retinoic Acid-Induced Differentiation of SH-SY5Y Cells: Neurotransmitters Phenotype Characterization
During brain development, neurite formation plays a critical role in neuronal communication and cognitive function. In the present study, we compared developmental changes in the expression of crucial markers that govern the functional activity of neurons, including somatostatin (SST), choline acety...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/2/337 |
| _version_ | 1827656714387718144 |
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| author | Sneha Singh Rishi K. Somvanshi Ujendra Kumar |
| author_facet | Sneha Singh Rishi K. Somvanshi Ujendra Kumar |
| author_sort | Sneha Singh |
| collection | DOAJ |
| description | During brain development, neurite formation plays a critical role in neuronal communication and cognitive function. In the present study, we compared developmental changes in the expression of crucial markers that govern the functional activity of neurons, including somatostatin (SST), choline acetyltransferase (ChAT), tyrosine hydroxylase (TH), brain nitric oxide synthase (bNOS), gamma-aminobutyric acid (GABA), glutamic acid decarboxylase (GAD-65) and synaptic vesicle protein synaptophysin (SYP) in non-differentiated and retinoic acid (RA)-induced differentiated SH-SY5Y cells. We further determined the role of SST in regulating subcellular distribution and expression of neurotransmitters. Our results indicate that SST potentiates RA-induced differentiation of SH-SY5Y cells and involves regulating the subcellular distribution and expression of neurotransmitter markers and synaptophysin translocation to neurites in a time-dependent manner, anticipating the therapeutic implication of SST in neurodegeneration. |
| first_indexed | 2024-03-09T22:33:13Z |
| format | Article |
| id | doaj.art-c63e5c06cb654bec8ac32cd8044f5eb5 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T22:33:13Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-c63e5c06cb654bec8ac32cd8044f5eb52023-11-23T18:54:02ZengMDPI AGBiomedicines2227-90592022-02-0110233710.3390/biomedicines10020337Somatostatin-Mediated Regulation of Retinoic Acid-Induced Differentiation of SH-SY5Y Cells: Neurotransmitters Phenotype CharacterizationSneha Singh0Rishi K. Somvanshi1Ujendra Kumar2Faculty of Pharmaceutical Sciences, The University of British Columbia, Vancouver, BC V6T 1Z3, CanadaFaculty of Pharmaceutical Sciences, The University of British Columbia, Vancouver, BC V6T 1Z3, CanadaFaculty of Pharmaceutical Sciences, The University of British Columbia, Vancouver, BC V6T 1Z3, CanadaDuring brain development, neurite formation plays a critical role in neuronal communication and cognitive function. In the present study, we compared developmental changes in the expression of crucial markers that govern the functional activity of neurons, including somatostatin (SST), choline acetyltransferase (ChAT), tyrosine hydroxylase (TH), brain nitric oxide synthase (bNOS), gamma-aminobutyric acid (GABA), glutamic acid decarboxylase (GAD-65) and synaptic vesicle protein synaptophysin (SYP) in non-differentiated and retinoic acid (RA)-induced differentiated SH-SY5Y cells. We further determined the role of SST in regulating subcellular distribution and expression of neurotransmitters. Our results indicate that SST potentiates RA-induced differentiation of SH-SY5Y cells and involves regulating the subcellular distribution and expression of neurotransmitter markers and synaptophysin translocation to neurites in a time-dependent manner, anticipating the therapeutic implication of SST in neurodegeneration.https://www.mdpi.com/2227-9059/10/2/337neurite outgrowthneurotransmitterretinoic acidSH-SY5Y cellssomatostatin |
| spellingShingle | Sneha Singh Rishi K. Somvanshi Ujendra Kumar Somatostatin-Mediated Regulation of Retinoic Acid-Induced Differentiation of SH-SY5Y Cells: Neurotransmitters Phenotype Characterization Biomedicines neurite outgrowth neurotransmitter retinoic acid SH-SY5Y cells somatostatin |
| title | Somatostatin-Mediated Regulation of Retinoic Acid-Induced Differentiation of SH-SY5Y Cells: Neurotransmitters Phenotype Characterization |
| title_full | Somatostatin-Mediated Regulation of Retinoic Acid-Induced Differentiation of SH-SY5Y Cells: Neurotransmitters Phenotype Characterization |
| title_fullStr | Somatostatin-Mediated Regulation of Retinoic Acid-Induced Differentiation of SH-SY5Y Cells: Neurotransmitters Phenotype Characterization |
| title_full_unstemmed | Somatostatin-Mediated Regulation of Retinoic Acid-Induced Differentiation of SH-SY5Y Cells: Neurotransmitters Phenotype Characterization |
| title_short | Somatostatin-Mediated Regulation of Retinoic Acid-Induced Differentiation of SH-SY5Y Cells: Neurotransmitters Phenotype Characterization |
| title_sort | somatostatin mediated regulation of retinoic acid induced differentiation of sh sy5y cells neurotransmitters phenotype characterization |
| topic | neurite outgrowth neurotransmitter retinoic acid SH-SY5Y cells somatostatin |
| url | https://www.mdpi.com/2227-9059/10/2/337 |
| work_keys_str_mv | AT snehasingh somatostatinmediatedregulationofretinoicacidinduceddifferentiationofshsy5ycellsneurotransmittersphenotypecharacterization AT rishiksomvanshi somatostatinmediatedregulationofretinoicacidinduceddifferentiationofshsy5ycellsneurotransmittersphenotypecharacterization AT ujendrakumar somatostatinmediatedregulationofretinoicacidinduceddifferentiationofshsy5ycellsneurotransmittersphenotypecharacterization |