BIN2 phosphorylates the Thr280 of CO to restrict its function in promoting Arabidopsis flowering
CONSTANS (CO) is a central regulator of floral initiation in response to photoperiod. In this study, we show that the GSK3 kinase BIN2 physically interacts with CO and the gain-of-function mutant bin2-1 displays late flowering phenotype through down-regulation of FT transcription. Genetic analyses s...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1068949/full |
| _version_ | 1811175362856484864 |
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| author | Lan Ju Huixue Dong Ruizhen Yang Yexing Jing Yunwei Zhang Liangyu Liu Yingfang Zhu Kun-Ming Chen Junai Ping Jiaqiang Sun |
| author_facet | Lan Ju Huixue Dong Ruizhen Yang Yexing Jing Yunwei Zhang Liangyu Liu Yingfang Zhu Kun-Ming Chen Junai Ping Jiaqiang Sun |
| author_sort | Lan Ju |
| collection | DOAJ |
| description | CONSTANS (CO) is a central regulator of floral initiation in response to photoperiod. In this study, we show that the GSK3 kinase BIN2 physically interacts with CO and the gain-of-function mutant bin2-1 displays late flowering phenotype through down-regulation of FT transcription. Genetic analyses show that BIN2 genetically acts upstream of CO in regulating flowering time. Further, we illustrate that BIN2 phosphorylates the Thr280 residue of CO. Importantly, the BIN2 phosphorylation of Thr280 residue restricts the function of CO in promoting flowering through affecting its DNA-binding activity. Moreover, we reveal that the N-terminal part of CO harboring the B-Box domain mediates the interaction of both CO-CO and BIN2-CO. We find that BIN2 inhibits the formation of CO dimer/oligomer. Taken together, this study reveals that BIN2 regulates flowering time through phosphorylating the Thr280 of CO and inhibiting the CO-CO interaction in Arabidopsis. |
| first_indexed | 2024-04-10T19:34:53Z |
| format | Article |
| id | doaj.art-c641e06ba0634c03a2b1c3efc9b03732 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T19:34:53Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-c641e06ba0634c03a2b1c3efc9b037322023-01-30T08:15:07ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-01-011410.3389/fpls.2023.10689491068949BIN2 phosphorylates the Thr280 of CO to restrict its function in promoting Arabidopsis floweringLan Ju0Huixue Dong1Ruizhen Yang2Yexing Jing3Yunwei Zhang4Liangyu Liu5Yingfang Zhu6Kun-Ming Chen7Junai Ping8Jiaqiang Sun9Shanxi Key Laboratory of Sorghum Genetic and Germplasm Innovation, Sorghum Research Institute, Shanxi Agricultural University, Jinzhong, ChinaNational Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaNational Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaNational Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaNational Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaBeijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, and College of Life Sciences, Capital Normal University, Beijing, ChinaState Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Life Sciences, Northwest A&F University, Yangling, ChinaShanxi Key Laboratory of Sorghum Genetic and Germplasm Innovation, Sorghum Research Institute, Shanxi Agricultural University, Jinzhong, ChinaNational Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, ChinaCONSTANS (CO) is a central regulator of floral initiation in response to photoperiod. In this study, we show that the GSK3 kinase BIN2 physically interacts with CO and the gain-of-function mutant bin2-1 displays late flowering phenotype through down-regulation of FT transcription. Genetic analyses show that BIN2 genetically acts upstream of CO in regulating flowering time. Further, we illustrate that BIN2 phosphorylates the Thr280 residue of CO. Importantly, the BIN2 phosphorylation of Thr280 residue restricts the function of CO in promoting flowering through affecting its DNA-binding activity. Moreover, we reveal that the N-terminal part of CO harboring the B-Box domain mediates the interaction of both CO-CO and BIN2-CO. We find that BIN2 inhibits the formation of CO dimer/oligomer. Taken together, this study reveals that BIN2 regulates flowering time through phosphorylating the Thr280 of CO and inhibiting the CO-CO interaction in Arabidopsis.https://www.frontiersin.org/articles/10.3389/fpls.2023.1068949/fullBIN2COfunctionphosphorylationfloweringArabidopsis |
| spellingShingle | Lan Ju Huixue Dong Ruizhen Yang Yexing Jing Yunwei Zhang Liangyu Liu Yingfang Zhu Kun-Ming Chen Junai Ping Jiaqiang Sun BIN2 phosphorylates the Thr280 of CO to restrict its function in promoting Arabidopsis flowering Frontiers in Plant Science BIN2 CO function phosphorylation flowering Arabidopsis |
| title | BIN2 phosphorylates the Thr280 of CO to restrict its function in promoting Arabidopsis flowering |
| title_full | BIN2 phosphorylates the Thr280 of CO to restrict its function in promoting Arabidopsis flowering |
| title_fullStr | BIN2 phosphorylates the Thr280 of CO to restrict its function in promoting Arabidopsis flowering |
| title_full_unstemmed | BIN2 phosphorylates the Thr280 of CO to restrict its function in promoting Arabidopsis flowering |
| title_short | BIN2 phosphorylates the Thr280 of CO to restrict its function in promoting Arabidopsis flowering |
| title_sort | bin2 phosphorylates the thr280 of co to restrict its function in promoting arabidopsis flowering |
| topic | BIN2 CO function phosphorylation flowering Arabidopsis |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1068949/full |
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