Okadaic Acid Toxin at Sublethal Dose Produced Cell Proliferation in Gastric and Colon Epithelial Cell Lines
The aim of this study was to analyze the effect of Okadaic Acid (OA) on the proliferation of gastric and colon epithelial cells, the main target tissues of the toxin. We hypothesized that OA, at sublethal doses, activates multiple signaling pathways, such as Erk and Akt, through the inhibition of PP...
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-12-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/11/12/4751 |
| _version_ | 1817990260599029760 |
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| author | Miguel del Campo Héctor Toledo Néstor Lagos |
| author_facet | Miguel del Campo Héctor Toledo Néstor Lagos |
| author_sort | Miguel del Campo |
| collection | DOAJ |
| description | The aim of this study was to analyze the effect of Okadaic Acid (OA) on the proliferation of gastric and colon epithelial cells, the main target tissues of the toxin. We hypothesized that OA, at sublethal doses, activates multiple signaling pathways, such as Erk and Akt, through the inhibition of PP2A. To demonstrate this, we carried out curves of doses and time response against OA in AGS, MKN-45 and Caco 2 cell lines, and found an increase in the cell proliferation at sublethal doses, at 24 h or 48 h exposure. Indeed, cells can withstand high concentrations of the toxin at 4 h exposure, the time chosen considering the maximum time before total gastric emptying. We have proved that this increased proliferation is due to an overexpression of Cyclin B, a cyclin that promotes the passage from G2 to mitosis. In addition, we have demonstrated that OA induces activation of Akt and Erk in the three cells lines, showing that OA can activate pathways involved in oncogenesis. In conclusion, this study contributes to the knowledge about the possible effects of chronic OA consumption. |
| first_indexed | 2024-04-14T00:56:36Z |
| format | Article |
| id | doaj.art-c64230b6526f43a68ae08ad2aaf6d87a |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-14T00:56:36Z |
| publishDate | 2013-12-01 |
| publisher | MDPI AG |
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| series | Marine Drugs |
| spelling | doaj.art-c64230b6526f43a68ae08ad2aaf6d87a2022-12-22T02:21:34ZengMDPI AGMarine Drugs1660-33972013-12-0111124751476010.3390/md11124751md11124751Okadaic Acid Toxin at Sublethal Dose Produced Cell Proliferation in Gastric and Colon Epithelial Cell LinesMiguel del Campo0Héctor Toledo1Néstor Lagos2Laboratory of Membrane Biochemistry, Program of Physiology and Biophysics, Faculty of Medicine, University of Chile, Independencia 1027, Correo 7, Santiago 8380000, ChileLaboratory of Molecular Microbiology, Program of Cellular and Molecular Biology, Faculty of Medicine, University of Chile, Independencia 1027, Correo 7, Santiago 8380000, ChileLaboratory of Membrane Biochemistry, Program of Physiology and Biophysics, Faculty of Medicine, University of Chile, Independencia 1027, Correo 7, Santiago 8380000, ChileThe aim of this study was to analyze the effect of Okadaic Acid (OA) on the proliferation of gastric and colon epithelial cells, the main target tissues of the toxin. We hypothesized that OA, at sublethal doses, activates multiple signaling pathways, such as Erk and Akt, through the inhibition of PP2A. To demonstrate this, we carried out curves of doses and time response against OA in AGS, MKN-45 and Caco 2 cell lines, and found an increase in the cell proliferation at sublethal doses, at 24 h or 48 h exposure. Indeed, cells can withstand high concentrations of the toxin at 4 h exposure, the time chosen considering the maximum time before total gastric emptying. We have proved that this increased proliferation is due to an overexpression of Cyclin B, a cyclin that promotes the passage from G2 to mitosis. In addition, we have demonstrated that OA induces activation of Akt and Erk in the three cells lines, showing that OA can activate pathways involved in oncogenesis. In conclusion, this study contributes to the knowledge about the possible effects of chronic OA consumption.http://www.mdpi.com/1660-3397/11/12/4751okadaic acidgastric cancercolon cancerPP2Aproliferation |
| spellingShingle | Miguel del Campo Héctor Toledo Néstor Lagos Okadaic Acid Toxin at Sublethal Dose Produced Cell Proliferation in Gastric and Colon Epithelial Cell Lines Marine Drugs okadaic acid gastric cancer colon cancer PP2A proliferation |
| title | Okadaic Acid Toxin at Sublethal Dose Produced Cell Proliferation in Gastric and Colon Epithelial Cell Lines |
| title_full | Okadaic Acid Toxin at Sublethal Dose Produced Cell Proliferation in Gastric and Colon Epithelial Cell Lines |
| title_fullStr | Okadaic Acid Toxin at Sublethal Dose Produced Cell Proliferation in Gastric and Colon Epithelial Cell Lines |
| title_full_unstemmed | Okadaic Acid Toxin at Sublethal Dose Produced Cell Proliferation in Gastric and Colon Epithelial Cell Lines |
| title_short | Okadaic Acid Toxin at Sublethal Dose Produced Cell Proliferation in Gastric and Colon Epithelial Cell Lines |
| title_sort | okadaic acid toxin at sublethal dose produced cell proliferation in gastric and colon epithelial cell lines |
| topic | okadaic acid gastric cancer colon cancer PP2A proliferation |
| url | http://www.mdpi.com/1660-3397/11/12/4751 |
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