The Obesity Amelioration Effect in High-Fat-Diet Fed Mice of a Homogeneous Polysaccharide from <i>Codonopsis pilosula</i>
A homogeneous polysaccharide coded as CPP−1 was extracted and purified from the root of <i>Codonopsis pilosula</i> (Franch.) Nannf. by water extraction, ethanol precipitation, and column chromatography. Its structure was analyzed by HPGPC-ELSD, HPLC, GC-MS, FT-IR, and NMR techniques. The...
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MDPI AG
2022-08-01
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| author | Qi Su Jiangyan Huo Yibin Wang Yang Zhou Dan Luo Jinjun Hou Zijia Zhang Huali Long Xianchun Zhong Cen Xie Min Lei Yameng Liu Wanying Wu |
| author_facet | Qi Su Jiangyan Huo Yibin Wang Yang Zhou Dan Luo Jinjun Hou Zijia Zhang Huali Long Xianchun Zhong Cen Xie Min Lei Yameng Liu Wanying Wu |
| author_sort | Qi Su |
| collection | DOAJ |
| description | A homogeneous polysaccharide coded as CPP−1 was extracted and purified from the root of <i>Codonopsis pilosula</i> (Franch.) Nannf. by water extraction, ethanol precipitation, and column chromatography. Its structure was analyzed by HPGPC-ELSD, HPLC, GC-MS, FT-IR, and NMR techniques. The results indicated that CPP−1 was composed of mannose (Man), glucose (Glc), galactose (Gal), and arabinose (Ara) at a molar ratio of 5.86 : 51.69 : 34.34 : 8.08. The methylation analysis revealed that the main glycosidic linkage types of CPP−1 were (1→)-linked-Glc residue, (1→3)-linked-Glc residues, (1→4)-linked-Gal residue, (1→2,3,4)-linked-Glc residue, (1→)-linked-Man residue, (1→3,4)-linked-Glc residue, and (1→)-linked-Ara residue. In vivo efficacy trial illustrated that CPP−1 supplements could alleviate HFD-induced mice obesity significantly, as well as improve obesity-induced disorders of glucose metabolism, alleviate insulin resistance, and improve the effects of lipid metabolism. The findings indicate that this polysaccharide has the potential for the treatment of obesity. |
| first_indexed | 2024-03-09T12:51:17Z |
| format | Article |
| id | doaj.art-c6425fca5eb94825b47c8d7ced0418ec |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T12:51:17Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c6425fca5eb94825b47c8d7ced0418ec2023-11-30T22:05:59ZengMDPI AGMolecules1420-30492022-08-012716534810.3390/molecules27165348The Obesity Amelioration Effect in High-Fat-Diet Fed Mice of a Homogeneous Polysaccharide from <i>Codonopsis pilosula</i>Qi Su0Jiangyan Huo1Yibin Wang2Yang Zhou3Dan Luo4Jinjun Hou5Zijia Zhang6Huali Long7Xianchun Zhong8Cen Xie9Min Lei10Yameng Liu11Wanying Wu12School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaShanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaSchool of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaA homogeneous polysaccharide coded as CPP−1 was extracted and purified from the root of <i>Codonopsis pilosula</i> (Franch.) Nannf. by water extraction, ethanol precipitation, and column chromatography. Its structure was analyzed by HPGPC-ELSD, HPLC, GC-MS, FT-IR, and NMR techniques. The results indicated that CPP−1 was composed of mannose (Man), glucose (Glc), galactose (Gal), and arabinose (Ara) at a molar ratio of 5.86 : 51.69 : 34.34 : 8.08. The methylation analysis revealed that the main glycosidic linkage types of CPP−1 were (1→)-linked-Glc residue, (1→3)-linked-Glc residues, (1→4)-linked-Gal residue, (1→2,3,4)-linked-Glc residue, (1→)-linked-Man residue, (1→3,4)-linked-Glc residue, and (1→)-linked-Ara residue. In vivo efficacy trial illustrated that CPP−1 supplements could alleviate HFD-induced mice obesity significantly, as well as improve obesity-induced disorders of glucose metabolism, alleviate insulin resistance, and improve the effects of lipid metabolism. The findings indicate that this polysaccharide has the potential for the treatment of obesity.https://www.mdpi.com/1420-3049/27/16/5348<i>Codonopsis pilosula</i>polysaccharidestructural characterizationobesity |
| spellingShingle | Qi Su Jiangyan Huo Yibin Wang Yang Zhou Dan Luo Jinjun Hou Zijia Zhang Huali Long Xianchun Zhong Cen Xie Min Lei Yameng Liu Wanying Wu The Obesity Amelioration Effect in High-Fat-Diet Fed Mice of a Homogeneous Polysaccharide from <i>Codonopsis pilosula</i> Molecules <i>Codonopsis pilosula</i> polysaccharide structural characterization obesity |
| title | The Obesity Amelioration Effect in High-Fat-Diet Fed Mice of a Homogeneous Polysaccharide from <i>Codonopsis pilosula</i> |
| title_full | The Obesity Amelioration Effect in High-Fat-Diet Fed Mice of a Homogeneous Polysaccharide from <i>Codonopsis pilosula</i> |
| title_fullStr | The Obesity Amelioration Effect in High-Fat-Diet Fed Mice of a Homogeneous Polysaccharide from <i>Codonopsis pilosula</i> |
| title_full_unstemmed | The Obesity Amelioration Effect in High-Fat-Diet Fed Mice of a Homogeneous Polysaccharide from <i>Codonopsis pilosula</i> |
| title_short | The Obesity Amelioration Effect in High-Fat-Diet Fed Mice of a Homogeneous Polysaccharide from <i>Codonopsis pilosula</i> |
| title_sort | obesity amelioration effect in high fat diet fed mice of a homogeneous polysaccharide from i codonopsis pilosula i |
| topic | <i>Codonopsis pilosula</i> polysaccharide structural characterization obesity |
| url | https://www.mdpi.com/1420-3049/27/16/5348 |
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