Development of a europium nanoparticles lateral flow immunoassay for NGAL detection in urine and diagnosis of acute kidney injury
Abstract Background AKI is related to severe adverse outcomes and mortality with Coronavirus Disease 2019 (COVID-19) patients, that early diagnosed and intervened is imperative. Neutrophil gelatinase-associated lipocalin (NGAL) is one of the most promising biomarkers for detection of acute kidney in...
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| Format: | Article |
| Language: | English |
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BMC
2022-01-01
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| Series: | BMC Nephrology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12882-021-02493-w |
| _version_ | 1819006830823604224 |
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| author | Moli Yin Yuanwang Nie Hao Liu Lei Liu Lu Tang Yuan Dong Chuanmin Hu Huiyan Wang |
| author_facet | Moli Yin Yuanwang Nie Hao Liu Lei Liu Lu Tang Yuan Dong Chuanmin Hu Huiyan Wang |
| author_sort | Moli Yin |
| collection | DOAJ |
| description | Abstract Background AKI is related to severe adverse outcomes and mortality with Coronavirus Disease 2019 (COVID-19) patients, that early diagnosed and intervened is imperative. Neutrophil gelatinase-associated lipocalin (NGAL) is one of the most promising biomarkers for detection of acute kidney injury (AKI), but current detection methods are inadequacy, so more rapid, convenient and accuracy methods are needed to detect NGAL for early diagnosis of AKI. Herein, we established a rapid, reliable and accuracy lateral flow immunoassay (LFIA) based on europium nanoparticles (EU-NPS) for the detection of NGAL in human urine specimens. Methods A double-antibody sandwich immunofluorescent assay using europium doped nanoparticles was employed and the NGAL monoclonal antibodies (MAbs) conjugate as labels were generated by optimizing electric fusion parameters. Eighty-three urine samples were used to evaluate the clinical application efficiency of this method. Results The quantitative detection range of NGAL in AKI was 1-3000 ng/mL, and the detection sensitization was 0.36 ng/mL. The coefficient of variation (CV) of intra-assay and inter-assay were 2.57-4.98 % and 4.11-7.83 %, respectively. Meanwhile, the correlation coefficient between europium nanoparticles-based lateral fluorescence immunoassays (EU-NPS-LFIA) and ARCHITECT analyzer was significant (R2 = 0.9829, n = 83, p < 0.01). Conclusions Thus, a faster and easier operation quantitative assay of NGAL for AKI has been established, which is very important and meaningful to diagnose the early AKI, suggesting that the assay can provide an early warning of final outcome of disease. |
| first_indexed | 2024-12-21T00:14:55Z |
| format | Article |
| id | doaj.art-c64360def7064feeadbf6a456df62aca |
| institution | Directory Open Access Journal |
| issn | 1471-2369 |
| language | English |
| last_indexed | 2024-12-21T00:14:55Z |
| publishDate | 2022-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Nephrology |
| spelling | doaj.art-c64360def7064feeadbf6a456df62aca2022-12-21T19:22:16ZengBMCBMC Nephrology1471-23692022-01-012311910.1186/s12882-021-02493-wDevelopment of a europium nanoparticles lateral flow immunoassay for NGAL detection in urine and diagnosis of acute kidney injuryMoli Yin0Yuanwang Nie1Hao Liu2Lei Liu3Lu Tang4Yuan Dong5Chuanmin Hu6Huiyan Wang7Jilin Collaborative Innovation Center for Antibody Engineering, Jilin Medical UniversityAcademy of laboratory, Jilin Medical UniversityAcademy of laboratory, Jilin Medical UniversityJilin Collaborative Innovation Center for Antibody Engineering, Jilin Medical UniversityJilin Collaborative Innovation Center for Antibody Engineering, Jilin Medical UniversityAcademy of laboratory, Jilin Medical UniversityJilin Collaborative Innovation Center for Antibody Engineering, Jilin Medical UniversityJilin Collaborative Innovation Center for Antibody Engineering, Jilin Medical UniversityAbstract Background AKI is related to severe adverse outcomes and mortality with Coronavirus Disease 2019 (COVID-19) patients, that early diagnosed and intervened is imperative. Neutrophil gelatinase-associated lipocalin (NGAL) is one of the most promising biomarkers for detection of acute kidney injury (AKI), but current detection methods are inadequacy, so more rapid, convenient and accuracy methods are needed to detect NGAL for early diagnosis of AKI. Herein, we established a rapid, reliable and accuracy lateral flow immunoassay (LFIA) based on europium nanoparticles (EU-NPS) for the detection of NGAL in human urine specimens. Methods A double-antibody sandwich immunofluorescent assay using europium doped nanoparticles was employed and the NGAL monoclonal antibodies (MAbs) conjugate as labels were generated by optimizing electric fusion parameters. Eighty-three urine samples were used to evaluate the clinical application efficiency of this method. Results The quantitative detection range of NGAL in AKI was 1-3000 ng/mL, and the detection sensitization was 0.36 ng/mL. The coefficient of variation (CV) of intra-assay and inter-assay were 2.57-4.98 % and 4.11-7.83 %, respectively. Meanwhile, the correlation coefficient between europium nanoparticles-based lateral fluorescence immunoassays (EU-NPS-LFIA) and ARCHITECT analyzer was significant (R2 = 0.9829, n = 83, p < 0.01). Conclusions Thus, a faster and easier operation quantitative assay of NGAL for AKI has been established, which is very important and meaningful to diagnose the early AKI, suggesting that the assay can provide an early warning of final outcome of disease.https://doi.org/10.1186/s12882-021-02493-wneutrophil gelatinase-associated lipocalin (NGAL)monoclonal antibodylateral flow immunoassayacute kidney injury (AKI) |
| spellingShingle | Moli Yin Yuanwang Nie Hao Liu Lei Liu Lu Tang Yuan Dong Chuanmin Hu Huiyan Wang Development of a europium nanoparticles lateral flow immunoassay for NGAL detection in urine and diagnosis of acute kidney injury BMC Nephrology neutrophil gelatinase-associated lipocalin (NGAL) monoclonal antibody lateral flow immunoassay acute kidney injury (AKI) |
| title | Development of a europium nanoparticles lateral flow immunoassay for NGAL detection in urine and diagnosis of acute kidney injury |
| title_full | Development of a europium nanoparticles lateral flow immunoassay for NGAL detection in urine and diagnosis of acute kidney injury |
| title_fullStr | Development of a europium nanoparticles lateral flow immunoassay for NGAL detection in urine and diagnosis of acute kidney injury |
| title_full_unstemmed | Development of a europium nanoparticles lateral flow immunoassay for NGAL detection in urine and diagnosis of acute kidney injury |
| title_short | Development of a europium nanoparticles lateral flow immunoassay for NGAL detection in urine and diagnosis of acute kidney injury |
| title_sort | development of a europium nanoparticles lateral flow immunoassay for ngal detection in urine and diagnosis of acute kidney injury |
| topic | neutrophil gelatinase-associated lipocalin (NGAL) monoclonal antibody lateral flow immunoassay acute kidney injury (AKI) |
| url | https://doi.org/10.1186/s12882-021-02493-w |
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