Proline Transport and Growth Changes in Proline Transport Mutants of <i>Staphylococcus aureus</i>
<i>Staphylococcus aureus</i> is a major cause of skin/soft tissue infections and more serious infections in humans. The species usually requires the importation of proline to be able to survive. Previous work has shown that single mutations in genes that encode for proline transporters a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/10/1888 |
| _version_ | 1797471189588770816 |
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| author | William R. Schwan |
| author_facet | William R. Schwan |
| author_sort | William R. Schwan |
| collection | DOAJ |
| description | <i>Staphylococcus aureus</i> is a major cause of skin/soft tissue infections and more serious infections in humans. The species usually requires the importation of proline to be able to survive. Previous work has shown that single mutations in genes that encode for proline transporters affect the ability of <i>S. aureus</i> to survive in vitro and in vivo. To better understand proline transport in <i>S. aureus</i>, double and triple gene mutant strains were created that targeted the <i>opuD</i>, <i>proP</i>, and <i>putP</i> genes. Single gene mutants had some effect on proline transport, whereas double mutants exhibited significantly lower proline transport. An <i>opuD prop putP</i> triple gene mutant displayed the lowest proline transport under low- and high-affinity conditions. To assess growth differences caused by the mutations, the same mutants were grown in brain heart infusion (BHI) broth and defined staphylococcal medium (DSM) with various concentrations of proline. The triple mutant did not grow in DSM with a low concentration of proline and grew poorly in both DSM with a high proline concentration and BHI broth. These results show that <i>S. aureus</i> has multiple mechanisms to import proline into the cell and knocking out three of the main proline transporters significantly hinders <i>S. aureus</i> growth. |
| first_indexed | 2024-03-09T19:45:43Z |
| format | Article |
| id | doaj.art-c64583e5082146a989b479a3d51cb12e |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T19:45:43Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-c64583e5082146a989b479a3d51cb12e2023-11-24T01:24:54ZengMDPI AGMicroorganisms2076-26072022-09-011010188810.3390/microorganisms10101888Proline Transport and Growth Changes in Proline Transport Mutants of <i>Staphylococcus aureus</i>William R. Schwan0Department of Microbiology, University of Wisconsin-La Crosse, 1725 State St., La Crosse, WI 54601, USA<i>Staphylococcus aureus</i> is a major cause of skin/soft tissue infections and more serious infections in humans. The species usually requires the importation of proline to be able to survive. Previous work has shown that single mutations in genes that encode for proline transporters affect the ability of <i>S. aureus</i> to survive in vitro and in vivo. To better understand proline transport in <i>S. aureus</i>, double and triple gene mutant strains were created that targeted the <i>opuD</i>, <i>proP</i>, and <i>putP</i> genes. Single gene mutants had some effect on proline transport, whereas double mutants exhibited significantly lower proline transport. An <i>opuD prop putP</i> triple gene mutant displayed the lowest proline transport under low- and high-affinity conditions. To assess growth differences caused by the mutations, the same mutants were grown in brain heart infusion (BHI) broth and defined staphylococcal medium (DSM) with various concentrations of proline. The triple mutant did not grow in DSM with a low concentration of proline and grew poorly in both DSM with a high proline concentration and BHI broth. These results show that <i>S. aureus</i> has multiple mechanisms to import proline into the cell and knocking out three of the main proline transporters significantly hinders <i>S. aureus</i> growth.https://www.mdpi.com/2076-2607/10/10/1888proline<i>Staphylococcus</i> <i>aureus</i>transportbacterial pathogenesis |
| spellingShingle | William R. Schwan Proline Transport and Growth Changes in Proline Transport Mutants of <i>Staphylococcus aureus</i> Microorganisms proline <i>Staphylococcus</i> <i>aureus</i> transport bacterial pathogenesis |
| title | Proline Transport and Growth Changes in Proline Transport Mutants of <i>Staphylococcus aureus</i> |
| title_full | Proline Transport and Growth Changes in Proline Transport Mutants of <i>Staphylococcus aureus</i> |
| title_fullStr | Proline Transport and Growth Changes in Proline Transport Mutants of <i>Staphylococcus aureus</i> |
| title_full_unstemmed | Proline Transport and Growth Changes in Proline Transport Mutants of <i>Staphylococcus aureus</i> |
| title_short | Proline Transport and Growth Changes in Proline Transport Mutants of <i>Staphylococcus aureus</i> |
| title_sort | proline transport and growth changes in proline transport mutants of i staphylococcus aureus i |
| topic | proline <i>Staphylococcus</i> <i>aureus</i> transport bacterial pathogenesis |
| url | https://www.mdpi.com/2076-2607/10/10/1888 |
| work_keys_str_mv | AT williamrschwan prolinetransportandgrowthchangesinprolinetransportmutantsofistaphylococcusaureusi |