| Summary: | <i>Nacobbus aberrans</i> ranks among the “top ten” plant-parasitic nematodes of phytosanitary importance. It causes significant losses in commercial interest crops in America and is a potential risk in the European Union. The nematicidal and phytotoxic activities of seven plant extracts against <i>N. aberrans</i> and <i>Solanum lycopersicum</i> were evaluated in vitro, respectively. The chemical nature of three nematicidal extracts (EC<sub>50,48h</sub> ≤ 113 µg mL<sup>−1</sup>) was studied through NMR analysis. Plant extracts showed nematicidal activity on second-stage juveniles (J2): (≥87%) at 1000 µg mL<sup>−1</sup> after 72 h, and their EC<sub>50</sub> values were 71.4–468.1 and 31.5–299.8 µg mL<sup>−1</sup> after 24 and 48 h, respectively. Extracts with the best nematicidal potential (EC<sub>50,48h</sub> < 113 µg mL<sup>−1</sup>) were those from <i>Adenophyllum aurantium</i>, <i>Alloispermum integrifolium</i>, and <i>Tournefortia densiflora</i>, which inhibited <i>L. esculentum</i> seed growth by 100% at 20 µg mL<sup>−1</sup>. Stigmasterol (<b>1</b>), β-sitosterol (<b>2</b>), and α-terthienyl (<b>3</b>) were identified from <i>A. aurantium</i>, while <b>1</b>, <b>2</b>, lutein (<b>4</b>), centaurin (<b>5</b>), patuletin-7-β-<i>O</i>-glucoside (<b>6</b>), pendulin (<b>7</b>), and penduletin (<b>8</b>) were identified from <i>A. integrifolium</i>. From <i>T. densiflora</i> extract, allantoin (<b>9</b>), 9-<i>O</i>-angeloyl-retronecine (<b>10</b>), and its N-oxide (<b>11</b>) were identified. The present research is the first to report the effect of <i>T. densiflora</i>, <i>A. integrifolium</i>, and <i>A. aurantium</i> against <i>N. aberrans</i> and chemically characterized nematicidal extracts that may provide alternative sources of botanical nematicides.
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