Comparison of PCR-based molecular markers for the characterization of Proteus mirabilis clinical isolates
Proteus mirabilis is one of the most important pathogens associated with complicated urinary tract infections (acute pyelonephritis, bladder infections, kidney stones) and bacteremia, affecting patients with anatomical abnormalities, immunodeficiency, and long-term urinary catheterization. For epide...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
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| Series: | Brazilian Journal of Infectious Diseases |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500014&lng=en&tlng=en |
| _version_ | 1819009140445413376 |
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| author | Lessandra Michelim Gabriela Muller Jucimar Zacaria Ana Paula Longaray Delamare Sérgio Olavo Pinto da Costa Sergio Echeverrigaray |
| author_facet | Lessandra Michelim Gabriela Muller Jucimar Zacaria Ana Paula Longaray Delamare Sérgio Olavo Pinto da Costa Sergio Echeverrigaray |
| author_sort | Lessandra Michelim |
| collection | DOAJ |
| description | Proteus mirabilis is one of the most important pathogens associated with complicated urinary tract infections (acute pyelonephritis, bladder infections, kidney stones) and bacteremia, affecting patients with anatomical abnormalities, immunodeficiency, and long-term urinary catheterization. For epidemiological purposes, various molecular typing methods, such as pulse-field gel electrophoresis (PFGE) or ribotyping, have been developed for this pathogen. However, these methods are labor intensive and time-consuming. We evaluated the discriminatory power of several PCR-based fingerprinting methods (RAPD, ISSR, ERIC-PCR, BOX-PCR and rep-PCR) for P. mirabilis clinical isolates. Typing patterns and clustering analysis indicated that RAPD, BOX-PCR and ERIC-PCR differentiated P. mirabilis strains from Escherichia coli, Hafnia alvei, and Morganella morganii. With the exception of rep-PCR, the methods gave medium to high discriminatory efficiency in P. mirabilis. In general, the results obtained with RAPD, BOX-PCR and ERIC-PCR were in good agreement. We concluded that a combination of ERIC-PCR and BOX-PCR results is a rapid and reliable alternative for discrimination among P. mirabilis clinical isolates, contributing to epidemiological studies. |
| first_indexed | 2024-12-21T00:51:38Z |
| format | Article |
| id | doaj.art-c647f107f06247b493ce2c4e25c908d6 |
| institution | Directory Open Access Journal |
| issn | 1678-4391 |
| language | English |
| last_indexed | 2024-12-21T00:51:38Z |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-c647f107f06247b493ce2c4e25c908d62022-12-21T19:21:24ZengElsevierBrazilian Journal of Infectious Diseases1678-439112542342910.1590/S1413-86702008000500014S1413-86702008000500014Comparison of PCR-based molecular markers for the characterization of Proteus mirabilis clinical isolatesLessandra Michelim0Gabriela Muller1Jucimar Zacaria2Ana Paula Longaray Delamare3Sérgio Olavo Pinto da Costa4Sergio Echeverrigaray5Universidade de Caxias do SulUniversidade de Caxias do SulUniversidade de Caxias do SulUniversidade de Caxias do SulUniversidade de Caxias do SulUniversidade de Caxias do SulProteus mirabilis is one of the most important pathogens associated with complicated urinary tract infections (acute pyelonephritis, bladder infections, kidney stones) and bacteremia, affecting patients with anatomical abnormalities, immunodeficiency, and long-term urinary catheterization. For epidemiological purposes, various molecular typing methods, such as pulse-field gel electrophoresis (PFGE) or ribotyping, have been developed for this pathogen. However, these methods are labor intensive and time-consuming. We evaluated the discriminatory power of several PCR-based fingerprinting methods (RAPD, ISSR, ERIC-PCR, BOX-PCR and rep-PCR) for P. mirabilis clinical isolates. Typing patterns and clustering analysis indicated that RAPD, BOX-PCR and ERIC-PCR differentiated P. mirabilis strains from Escherichia coli, Hafnia alvei, and Morganella morganii. With the exception of rep-PCR, the methods gave medium to high discriminatory efficiency in P. mirabilis. In general, the results obtained with RAPD, BOX-PCR and ERIC-PCR were in good agreement. We concluded that a combination of ERIC-PCR and BOX-PCR results is a rapid and reliable alternative for discrimination among P. mirabilis clinical isolates, contributing to epidemiological studies.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500014&lng=en&tlng=enProteus mirabilismolecular markersfingerprintingPCR |
| spellingShingle | Lessandra Michelim Gabriela Muller Jucimar Zacaria Ana Paula Longaray Delamare Sérgio Olavo Pinto da Costa Sergio Echeverrigaray Comparison of PCR-based molecular markers for the characterization of Proteus mirabilis clinical isolates Brazilian Journal of Infectious Diseases Proteus mirabilis molecular markers fingerprinting PCR |
| title | Comparison of PCR-based molecular markers for the characterization of Proteus mirabilis clinical isolates |
| title_full | Comparison of PCR-based molecular markers for the characterization of Proteus mirabilis clinical isolates |
| title_fullStr | Comparison of PCR-based molecular markers for the characterization of Proteus mirabilis clinical isolates |
| title_full_unstemmed | Comparison of PCR-based molecular markers for the characterization of Proteus mirabilis clinical isolates |
| title_short | Comparison of PCR-based molecular markers for the characterization of Proteus mirabilis clinical isolates |
| title_sort | comparison of pcr based molecular markers for the characterization of proteus mirabilis clinical isolates |
| topic | Proteus mirabilis molecular markers fingerprinting PCR |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500014&lng=en&tlng=en |
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