Extraction, characterization of polyphenols from certain medicinal plants and evaluation of their antioxidant, antitumor, antidiabetic, antimicrobial properties, and potential use in human nutrition
IntroductionDietary medicinal plants are among the most sought-after topics in alternative medicine today due to their preventive and healing properties against many diseases.AimThis study aimed to extract and determine the polyphenols from indigenous plants extracts, i.e., Mentha longifolia, M. arv...
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2023.1125106/full |
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| author | Abdul Mueed Abdul Mueed Sahar Shibli Diana A. Al-Quwaie Mada F. Ashkan Mona Alharbi Humidah Alanazi Najat Binothman Majidah Aljadani Kamlah Ali Majrashi Mashael Huwaikem Mohammed A. S. Abourehab Mohammed A. S. Abourehab Sameh A. Korma Mohamed T. El-Saadony |
| author_facet | Abdul Mueed Abdul Mueed Sahar Shibli Diana A. Al-Quwaie Mada F. Ashkan Mona Alharbi Humidah Alanazi Najat Binothman Majidah Aljadani Kamlah Ali Majrashi Mashael Huwaikem Mohammed A. S. Abourehab Mohammed A. S. Abourehab Sameh A. Korma Mohamed T. El-Saadony |
| author_sort | Abdul Mueed |
| collection | DOAJ |
| description | IntroductionDietary medicinal plants are among the most sought-after topics in alternative medicine today due to their preventive and healing properties against many diseases.AimThis study aimed to extract and determine the polyphenols from indigenous plants extracts, i.e., Mentha longifolia, M. arvensis, Tinospora cordifolia, Cymbopogon citratus, Foeniculum vulgare, Cassia absus, Camellia sinensis, Trachyspermum ammi, C. sinensis and M. arvensis, then evaluate the antioxidant, cytotoxicity, and antimicrobial properties, besides enzyme inhibition of isolated polyphenols.MethodsThe antioxidant activity was evaluated by DPPH, Superoxide radical, Hydroxyl radical (OH.), and Nitric oxide (NO.) scavenging activity; the antidiabetic activity was evaluated by enzymatic methods, and anticancer activity using MTT assay, while the antibacterial activity.ResultsThe results showed that tested medicinal plants’ polyphenolic extracts (MPPE) exhibited the most significant antioxidant activity in DPPH, hydroxyl, nitric oxide, and superoxide radical scavenging methods because of the considerable amounts of total polyphenol and flavonoid contents. UHPLC profile showed twenty-five polyphenol complexes in eight medicinal plant extracts, categorized into phenolic acids, flavonoids, and alkaloids. The main polyphenol was 3-Feroylquinic acid (1,302 mg/L), also found in M. longifolia, C. absus, and C. sinensis, has a higher phenolic content, i.e., rosmarinic acid, vanillic acid, chlorogenic acid, p-coumaric acid, ferulic acid, gallic acid, catechin, luteolin, 7-O-neohesperideside, quercetin 3,7-O-glucoside, hesperidin, rutin, quercetin, and caffeine in the range of (560–780 mg/L). At the same time, other compounds are of medium content (99–312 mg/L). The phenolics in C. sinensis were 20–116% more abundant than those in M. longifolia, C. absus, and other medicinal plants. While T. cordifolia is rich in alkaloids, T. ammi has a lower content. The MTT assay against Caco-2 cells showed that polyphenolic extracts of T. ammi and C. citratus had maximum cytotoxicity. While M. arvensis, C. sinensis, and F. vulgare extracts showed significant enzyme inhibition activity, C. sinensis showed minor inhibition activity against α-amylase. Furthermore, F. vulgare and C. sinensis polyphenolic extracts showed considerable antibacterial activity against S. aureus, B. cereus, E. coli, and S. enterica.DiscussionThe principal component analysis demonstrated clear separation among medicinal plants’ extracts based on their functional properties. These findings prove the therapeutic effectiveness of indigenous plants and highlight their importance as natural reserves of phytogenic compounds with untapped potential that needs to be discovered through advanced analytical methods. |
| first_indexed | 2024-03-13T04:05:39Z |
| format | Article |
| id | doaj.art-c6499ecdcba1464c89896d53a39ad39c |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-03-13T04:05:39Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Nutrition |
| spelling | doaj.art-c6499ecdcba1464c89896d53a39ad39c2023-06-21T08:18:59ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2023-06-011010.3389/fnut.2023.11251061125106Extraction, characterization of polyphenols from certain medicinal plants and evaluation of their antioxidant, antitumor, antidiabetic, antimicrobial properties, and potential use in human nutritionAbdul Mueed0Abdul Mueed1Sahar Shibli2Diana A. Al-Quwaie3Mada F. Ashkan4Mona Alharbi5Humidah Alanazi6Najat Binothman7Majidah Aljadani8Kamlah Ali Majrashi9Mashael Huwaikem10Mohammed A. S. Abourehab11Mohammed A. S. Abourehab12Sameh A. Korma13Mohamed T. El-Saadony14State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi, ChinaDepartment of Food Technology, Institute of Food and Nutrition, Arid Agriculture University, Rawalpindi, PakistanFood Science Research Institute, National Agricultural Research Centre, Islamabad, PakistanBiological Sciences Department, College of Science & Arts, King Abdulaziz University, Rabigh, Saudi ArabiaBiological Sciences Department, College of Science & Arts, King Abdulaziz University, Rabigh, Saudi ArabiaDepartment of Biochemistry, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Biochemistry, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Chemistry, College of Sciences & Arts, King Abdulaziz University, Rabigh, Saudi ArabiaDepartment of Chemistry, College of Sciences & Arts, King Abdulaziz University, Rabigh, Saudi ArabiaBiological Sciences Department, College of Science & Arts, King Abdulaziz University, Rabigh, Saudi ArabiaCinical Nutrition Department, College of Applied Medical Sciences, King Faisal University, Al Ahsa, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, Umm Al-Qura University, Makkah, Saudi ArabiaDepartment of Pharmaceutics and Industrial Pharmacy, College of Pharmacy, Minia University, Minia, Egypt0Department of Food Science, Faculty of Agriculture, Zagazig University, Zagazig, Egypt1Department of Agricultural Microbiology, Faculty of Agriculture, Zagazig University, Zagazig, EgyptIntroductionDietary medicinal plants are among the most sought-after topics in alternative medicine today due to their preventive and healing properties against many diseases.AimThis study aimed to extract and determine the polyphenols from indigenous plants extracts, i.e., Mentha longifolia, M. arvensis, Tinospora cordifolia, Cymbopogon citratus, Foeniculum vulgare, Cassia absus, Camellia sinensis, Trachyspermum ammi, C. sinensis and M. arvensis, then evaluate the antioxidant, cytotoxicity, and antimicrobial properties, besides enzyme inhibition of isolated polyphenols.MethodsThe antioxidant activity was evaluated by DPPH, Superoxide radical, Hydroxyl radical (OH.), and Nitric oxide (NO.) scavenging activity; the antidiabetic activity was evaluated by enzymatic methods, and anticancer activity using MTT assay, while the antibacterial activity.ResultsThe results showed that tested medicinal plants’ polyphenolic extracts (MPPE) exhibited the most significant antioxidant activity in DPPH, hydroxyl, nitric oxide, and superoxide radical scavenging methods because of the considerable amounts of total polyphenol and flavonoid contents. UHPLC profile showed twenty-five polyphenol complexes in eight medicinal plant extracts, categorized into phenolic acids, flavonoids, and alkaloids. The main polyphenol was 3-Feroylquinic acid (1,302 mg/L), also found in M. longifolia, C. absus, and C. sinensis, has a higher phenolic content, i.e., rosmarinic acid, vanillic acid, chlorogenic acid, p-coumaric acid, ferulic acid, gallic acid, catechin, luteolin, 7-O-neohesperideside, quercetin 3,7-O-glucoside, hesperidin, rutin, quercetin, and caffeine in the range of (560–780 mg/L). At the same time, other compounds are of medium content (99–312 mg/L). The phenolics in C. sinensis were 20–116% more abundant than those in M. longifolia, C. absus, and other medicinal plants. While T. cordifolia is rich in alkaloids, T. ammi has a lower content. The MTT assay against Caco-2 cells showed that polyphenolic extracts of T. ammi and C. citratus had maximum cytotoxicity. While M. arvensis, C. sinensis, and F. vulgare extracts showed significant enzyme inhibition activity, C. sinensis showed minor inhibition activity against α-amylase. Furthermore, F. vulgare and C. sinensis polyphenolic extracts showed considerable antibacterial activity against S. aureus, B. cereus, E. coli, and S. enterica.DiscussionThe principal component analysis demonstrated clear separation among medicinal plants’ extracts based on their functional properties. These findings prove the therapeutic effectiveness of indigenous plants and highlight their importance as natural reserves of phytogenic compounds with untapped potential that needs to be discovered through advanced analytical methods.https://www.frontiersin.org/articles/10.3389/fnut.2023.1125106/fullmedicinal plantspolyphenolsantioxidantantidiabeticantitumorantimicrobial |
| spellingShingle | Abdul Mueed Abdul Mueed Sahar Shibli Diana A. Al-Quwaie Mada F. Ashkan Mona Alharbi Humidah Alanazi Najat Binothman Majidah Aljadani Kamlah Ali Majrashi Mashael Huwaikem Mohammed A. S. Abourehab Mohammed A. S. Abourehab Sameh A. Korma Mohamed T. El-Saadony Extraction, characterization of polyphenols from certain medicinal plants and evaluation of their antioxidant, antitumor, antidiabetic, antimicrobial properties, and potential use in human nutrition Frontiers in Nutrition medicinal plants polyphenols antioxidant antidiabetic antitumor antimicrobial |
| title | Extraction, characterization of polyphenols from certain medicinal plants and evaluation of their antioxidant, antitumor, antidiabetic, antimicrobial properties, and potential use in human nutrition |
| title_full | Extraction, characterization of polyphenols from certain medicinal plants and evaluation of their antioxidant, antitumor, antidiabetic, antimicrobial properties, and potential use in human nutrition |
| title_fullStr | Extraction, characterization of polyphenols from certain medicinal plants and evaluation of their antioxidant, antitumor, antidiabetic, antimicrobial properties, and potential use in human nutrition |
| title_full_unstemmed | Extraction, characterization of polyphenols from certain medicinal plants and evaluation of their antioxidant, antitumor, antidiabetic, antimicrobial properties, and potential use in human nutrition |
| title_short | Extraction, characterization of polyphenols from certain medicinal plants and evaluation of their antioxidant, antitumor, antidiabetic, antimicrobial properties, and potential use in human nutrition |
| title_sort | extraction characterization of polyphenols from certain medicinal plants and evaluation of their antioxidant antitumor antidiabetic antimicrobial properties and potential use in human nutrition |
| topic | medicinal plants polyphenols antioxidant antidiabetic antitumor antimicrobial |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2023.1125106/full |
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