Decoding chikungunya virus non-structural protein 3 interacting partners in THP-1 derived infected macrophages through proteomic profiling
IntroductionChikungunya virus (CHIKV), an alphavirus transmitted by mosquitoes, has instigated several epidemics in recent years, sparking intensive efforts to understand its biology. Despite progress, the understanding of CHIKV’s molecular interactions with host cell constituents, especially in sus...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Virology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fviro.2024.1310161/full |
| _version_ | 1797263084240240640 |
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| author | Priyanshu Srivastava Nimisha Mishra Sakshi Chaudhary Sujatha Sunil |
| author_facet | Priyanshu Srivastava Nimisha Mishra Sakshi Chaudhary Sujatha Sunil |
| author_sort | Priyanshu Srivastava |
| collection | DOAJ |
| description | IntroductionChikungunya virus (CHIKV), an alphavirus transmitted by mosquitoes, has instigated several epidemics in recent years, sparking intensive efforts to understand its biology. Despite progress, the understanding of CHIKV’s molecular interactions with host cell constituents, especially in susceptible cells such as macrophages remains limited.MethodsWe used a mass spectrometry platform to characterize the interactions between CHIKV-nsP3, a viral nonstructural protein, and proteins in human THP-1 macrophage cells.Results and DiscussionOur findings revealed 196 high-confidence interactions primarily involving nsP3. Further, the sub-cellular localization, and pathways these interacting proteins might be involved in were deduced using computational methods. The interacting partners were further incorporated into a comprehensive host-virus interaction network derived from extensive literature on alphavirus-host interactions. Collectively, this study offers the first interaction map between CHIKV nsP3 protein and THP-1 cells, illuminating new probable roles of host cell proteins in CHIKV’s replication cycle. |
| first_indexed | 2024-04-25T00:07:23Z |
| format | Article |
| id | doaj.art-c64d9cf86d4041a68701ef7dbcb49ce0 |
| institution | Directory Open Access Journal |
| issn | 2673-818X |
| language | English |
| last_indexed | 2024-04-25T00:07:23Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Virology |
| spelling | doaj.art-c64d9cf86d4041a68701ef7dbcb49ce02024-03-14T05:03:00ZengFrontiers Media S.A.Frontiers in Virology2673-818X2024-03-01410.3389/fviro.2024.13101611310161Decoding chikungunya virus non-structural protein 3 interacting partners in THP-1 derived infected macrophages through proteomic profilingPriyanshu SrivastavaNimisha MishraSakshi ChaudharySujatha SunilIntroductionChikungunya virus (CHIKV), an alphavirus transmitted by mosquitoes, has instigated several epidemics in recent years, sparking intensive efforts to understand its biology. Despite progress, the understanding of CHIKV’s molecular interactions with host cell constituents, especially in susceptible cells such as macrophages remains limited.MethodsWe used a mass spectrometry platform to characterize the interactions between CHIKV-nsP3, a viral nonstructural protein, and proteins in human THP-1 macrophage cells.Results and DiscussionOur findings revealed 196 high-confidence interactions primarily involving nsP3. Further, the sub-cellular localization, and pathways these interacting proteins might be involved in were deduced using computational methods. The interacting partners were further incorporated into a comprehensive host-virus interaction network derived from extensive literature on alphavirus-host interactions. Collectively, this study offers the first interaction map between CHIKV nsP3 protein and THP-1 cells, illuminating new probable roles of host cell proteins in CHIKV’s replication cycle.https://www.frontiersin.org/articles/10.3389/fviro.2024.1310161/fullchikungunya virus (CHIKV)macrophagesnon-structural protein 3proteomeTHP1 |
| spellingShingle | Priyanshu Srivastava Nimisha Mishra Sakshi Chaudhary Sujatha Sunil Decoding chikungunya virus non-structural protein 3 interacting partners in THP-1 derived infected macrophages through proteomic profiling Frontiers in Virology chikungunya virus (CHIKV) macrophages non-structural protein 3 proteome THP1 |
| title | Decoding chikungunya virus non-structural protein 3 interacting partners in THP-1 derived infected macrophages through proteomic profiling |
| title_full | Decoding chikungunya virus non-structural protein 3 interacting partners in THP-1 derived infected macrophages through proteomic profiling |
| title_fullStr | Decoding chikungunya virus non-structural protein 3 interacting partners in THP-1 derived infected macrophages through proteomic profiling |
| title_full_unstemmed | Decoding chikungunya virus non-structural protein 3 interacting partners in THP-1 derived infected macrophages through proteomic profiling |
| title_short | Decoding chikungunya virus non-structural protein 3 interacting partners in THP-1 derived infected macrophages through proteomic profiling |
| title_sort | decoding chikungunya virus non structural protein 3 interacting partners in thp 1 derived infected macrophages through proteomic profiling |
| topic | chikungunya virus (CHIKV) macrophages non-structural protein 3 proteome THP1 |
| url | https://www.frontiersin.org/articles/10.3389/fviro.2024.1310161/full |
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