An optimized protocol for the retroviral transduction of mouse CD4 T cells
Summary: Transduction of primary T cells has become prominent with the introduction of chimeric antigen receptor T-cell therapy. Although there are many protocols for the transduction of human T cells, it remains a challenge to transduce murine T cells. We present an optimized protocol for the retro...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721004263 |
| _version_ | 1818358834438078464 |
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| author | Ekaterina Eremenko Zoe V. Taylor Bishnu Khand Shir Zaccai Angel Porgador Alon Monsonego |
| author_facet | Ekaterina Eremenko Zoe V. Taylor Bishnu Khand Shir Zaccai Angel Porgador Alon Monsonego |
| author_sort | Ekaterina Eremenko |
| collection | DOAJ |
| description | Summary: Transduction of primary T cells has become prominent with the introduction of chimeric antigen receptor T-cell therapy. Although there are many protocols for the transduction of human T cells, it remains a challenge to transduce murine T cells. We present an optimized protocol for the retroviral transduction of murine CD4 T cells, which overcomes major challenges including large-scale production and long-term culturing of transduced cells. The optimized protocol combines high transduction efficiency with a low rate of cell death.For complete details on the use and execution of this protocol, please refer to Eremenko et al., 2019. |
| first_indexed | 2024-12-13T20:35:18Z |
| format | Article |
| id | doaj.art-c64dd8db782a4cf282175d61248356e4 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-13T20:35:18Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-c64dd8db782a4cf282175d61248356e42022-12-21T23:32:19ZengElsevierSTAR Protocols2666-16672021-09-0123100719An optimized protocol for the retroviral transduction of mouse CD4 T cellsEkaterina Eremenko0Zoe V. Taylor1Bishnu Khand2Shir Zaccai3Angel Porgador4Alon Monsonego5The Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The Department of Life Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The National Institute of Biotechnology in the Negev, Zlotowski Neuroscience Center, and Regenerative Medicine and Stem Cell Research Center, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; Corresponding authorThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The National Institute of Biotechnology in the Negev, Zlotowski Neuroscience Center, and Regenerative Medicine and Stem Cell Research Center, Ben-Gurion University of the Negev, Beer Sheva 8410501, IsraelThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The National Institute of Biotechnology in the Negev, Zlotowski Neuroscience Center, and Regenerative Medicine and Stem Cell Research Center, Ben-Gurion University of the Negev, Beer Sheva 8410501, IsraelThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The Department of Life Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The National Institute of Biotechnology in the Negev, Zlotowski Neuroscience Center, and Regenerative Medicine and Stem Cell Research Center, Ben-Gurion University of the Negev, Beer Sheva 8410501, IsraelThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The National Institute of Biotechnology in the Negev, Zlotowski Neuroscience Center, and Regenerative Medicine and Stem Cell Research Center, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; Corresponding authorThe Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The Department of Physiology and Cell Biology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; The National Institute of Biotechnology in the Negev, Zlotowski Neuroscience Center, and Regenerative Medicine and Stem Cell Research Center, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel; Corresponding authorSummary: Transduction of primary T cells has become prominent with the introduction of chimeric antigen receptor T-cell therapy. Although there are many protocols for the transduction of human T cells, it remains a challenge to transduce murine T cells. We present an optimized protocol for the retroviral transduction of murine CD4 T cells, which overcomes major challenges including large-scale production and long-term culturing of transduced cells. The optimized protocol combines high transduction efficiency with a low rate of cell death.For complete details on the use and execution of this protocol, please refer to Eremenko et al., 2019.http://www.sciencedirect.com/science/article/pii/S2666166721004263Cell cultureCell-based AssaysCell separation/fractionationImmunologyMolecular Biology |
| spellingShingle | Ekaterina Eremenko Zoe V. Taylor Bishnu Khand Shir Zaccai Angel Porgador Alon Monsonego An optimized protocol for the retroviral transduction of mouse CD4 T cells STAR Protocols Cell culture Cell-based Assays Cell separation/fractionation Immunology Molecular Biology |
| title | An optimized protocol for the retroviral transduction of mouse CD4 T cells |
| title_full | An optimized protocol for the retroviral transduction of mouse CD4 T cells |
| title_fullStr | An optimized protocol for the retroviral transduction of mouse CD4 T cells |
| title_full_unstemmed | An optimized protocol for the retroviral transduction of mouse CD4 T cells |
| title_short | An optimized protocol for the retroviral transduction of mouse CD4 T cells |
| title_sort | optimized protocol for the retroviral transduction of mouse cd4 t cells |
| topic | Cell culture Cell-based Assays Cell separation/fractionation Immunology Molecular Biology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721004263 |
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