Quantitative Analysis of Nuclear Lamins Imaged by Super-Resolution Light Microscopy
The nuclear lamina consists of a dense fibrous meshwork of nuclear lamins, Type V intermediate filaments, and is ~14 nm thick according to recent cryo-electron tomography studies. Recent advances in light microscopy have extended the resolution to a scale allowing for the fine structure of the lamin...
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| Format: | Article |
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MDPI AG
2019-04-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/8/4/361 |
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| author | Mark Kittisopikul Laura Virtanen Pekka Taimen Robert D. Goldman |
| author_facet | Mark Kittisopikul Laura Virtanen Pekka Taimen Robert D. Goldman |
| author_sort | Mark Kittisopikul |
| collection | DOAJ |
| description | The nuclear lamina consists of a dense fibrous meshwork of nuclear lamins, Type V intermediate filaments, and is ~14 nm thick according to recent cryo-electron tomography studies. Recent advances in light microscopy have extended the resolution to a scale allowing for the fine structure of the lamina to be imaged in the context of the whole nucleus. We review quantitative approaches to analyze the imaging data of the nuclear lamina as acquired by structured illumination microscopy (SIM) and single molecule localization microscopy (SMLM), as well as the requisite cell preparation techniques. In particular, we discuss the application of steerable filters and graph-based methods to segment the structure of the four mammalian lamin isoforms (A, C, B1, and B2) and extract quantitative information. |
| first_indexed | 2024-03-12T07:05:32Z |
| format | Article |
| id | doaj.art-c653fd0c32264b33aa06643e0647a78a |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T07:05:32Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-c653fd0c32264b33aa06643e0647a78a2023-09-02T23:28:23ZengMDPI AGCells2073-44092019-04-018436110.3390/cells8040361cells8040361Quantitative Analysis of Nuclear Lamins Imaged by Super-Resolution Light MicroscopyMark Kittisopikul0Laura Virtanen1Pekka Taimen2Robert D. Goldman3Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USAInstitute of Biomedicine, Research Center for Cancer, Infections and Immunity, University of Turku, 20520 Turku, FinlandInstitute of Biomedicine, Research Center for Cancer, Infections and Immunity, University of Turku, 20520 Turku, FinlandDepartment of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USAThe nuclear lamina consists of a dense fibrous meshwork of nuclear lamins, Type V intermediate filaments, and is ~14 nm thick according to recent cryo-electron tomography studies. Recent advances in light microscopy have extended the resolution to a scale allowing for the fine structure of the lamina to be imaged in the context of the whole nucleus. We review quantitative approaches to analyze the imaging data of the nuclear lamina as acquired by structured illumination microscopy (SIM) and single molecule localization microscopy (SMLM), as well as the requisite cell preparation techniques. In particular, we discuss the application of steerable filters and graph-based methods to segment the structure of the four mammalian lamin isoforms (A, C, B1, and B2) and extract quantitative information.https://www.mdpi.com/2073-4409/8/4/361laminsstructured illumination microscopysingle molecule localization microscopysteerable filterscomputational geometrydelaunay triangulationvoronoi tessellation |
| spellingShingle | Mark Kittisopikul Laura Virtanen Pekka Taimen Robert D. Goldman Quantitative Analysis of Nuclear Lamins Imaged by Super-Resolution Light Microscopy Cells lamins structured illumination microscopy single molecule localization microscopy steerable filters computational geometry delaunay triangulation voronoi tessellation |
| title | Quantitative Analysis of Nuclear Lamins Imaged by Super-Resolution Light Microscopy |
| title_full | Quantitative Analysis of Nuclear Lamins Imaged by Super-Resolution Light Microscopy |
| title_fullStr | Quantitative Analysis of Nuclear Lamins Imaged by Super-Resolution Light Microscopy |
| title_full_unstemmed | Quantitative Analysis of Nuclear Lamins Imaged by Super-Resolution Light Microscopy |
| title_short | Quantitative Analysis of Nuclear Lamins Imaged by Super-Resolution Light Microscopy |
| title_sort | quantitative analysis of nuclear lamins imaged by super resolution light microscopy |
| topic | lamins structured illumination microscopy single molecule localization microscopy steerable filters computational geometry delaunay triangulation voronoi tessellation |
| url | https://www.mdpi.com/2073-4409/8/4/361 |
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