Nuclear conditions of basidiospores and hyphal cells in the edible mushroom Oudemansiella aparlosarca
Abstract Oudemansiella aparlosarca is an edible mushroom possessing medicinal and health benefits. Although there are studies on the cultivation of O. aparlosarca, only a few studies have focused on its genetics and life cycle. Therefore, the main objective of this study was to identify the nuclear...
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| Format: | Article |
| Language: | English |
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Wiley
2021-10-01
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| Series: | MicrobiologyOpen |
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| Online Access: | https://doi.org/10.1002/mbo3.1233 |
| _version_ | 1818925919431032832 |
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| author | Roy Rebecca Qi Gao Yujin Cui Chengbo Rong Yu Liu Wensheng Zhao Wasantha Kumara Shouxian Wang |
| author_facet | Roy Rebecca Qi Gao Yujin Cui Chengbo Rong Yu Liu Wensheng Zhao Wasantha Kumara Shouxian Wang |
| author_sort | Roy Rebecca |
| collection | DOAJ |
| description | Abstract Oudemansiella aparlosarca is an edible mushroom possessing medicinal and health benefits. Although there are studies on the cultivation of O. aparlosarca, only a few studies have focused on its genetics and life cycle. Therefore, the main objective of this study was to identify the nuclear conditions of basidiospores and homokaryotic and heterokaryotic hyphal cells and to determine the influence of different nuclear conditions on basidiospore diameter in O. aparlosarca. Two parental strains: strain‐55 and strain‐81 were used. Staining of basidiospores and hyphal cells in the apical region was performed. We observed the following nuclear conditions: non‐nucleate, mononucleate, binucleate, and multinucleate. In both parental strains, binucleate spores were predominant, while the number of non‐nucleate spores was the lowest. The diameter of non‐nucleate spores was the smallest, being 11.52 µm and 12.15 µm in parental strain‐81 and strain‐55, respectively, while multinucleate spores had the largest diameter, being 14.78 µm in both parental strains. Both homokaryotic and heterokaryotic strains were identified in isolated single spores from parental strains. Binucleate cells were majorly present in heterokaryotic hyphal cells, and multinucleate cells were predominant in homokaryotic hyphal cells. We conclude that O. aparlosarca contains homokaryotic and heterokaryotic basidiospores, which indicates an amphithallic life cycle. The observed binucleate spores might be the result of post‐meiotic mitosis. |
| first_indexed | 2024-12-20T02:48:52Z |
| format | Article |
| id | doaj.art-c6546bcad4ac4d54afd711c177925c6d |
| institution | Directory Open Access Journal |
| issn | 2045-8827 |
| language | English |
| last_indexed | 2024-12-20T02:48:52Z |
| publishDate | 2021-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | MicrobiologyOpen |
| spelling | doaj.art-c6546bcad4ac4d54afd711c177925c6d2022-12-21T19:56:06ZengWileyMicrobiologyOpen2045-88272021-10-01105n/an/a10.1002/mbo3.1233Nuclear conditions of basidiospores and hyphal cells in the edible mushroom Oudemansiella aparlosarcaRoy Rebecca0Qi Gao1Yujin Cui2Chengbo Rong3Yu Liu4Wensheng Zhao5Wasantha Kumara6Shouxian Wang7Key Laboratory of Urban Agriculture (North) Institute of Plant Protection Beijing Academy of Agriculture and Forestry Sciences Beijing Engineering Research Center for Edible Mushroom Ministry of Agriculture Beijing ChinaKey Laboratory of Urban Agriculture (North) Institute of Plant Protection Beijing Academy of Agriculture and Forestry Sciences Beijing Engineering Research Center for Edible Mushroom Ministry of Agriculture Beijing ChinaKey Laboratory of Urban Agriculture (North) Institute of Plant Protection Beijing Academy of Agriculture and Forestry Sciences Beijing Engineering Research Center for Edible Mushroom Ministry of Agriculture Beijing ChinaKey Laboratory of Urban Agriculture (North) Institute of Plant Protection Beijing Academy of Agriculture and Forestry Sciences Beijing Engineering Research Center for Edible Mushroom Ministry of Agriculture Beijing ChinaKey Laboratory of Urban Agriculture (North) Institute of Plant Protection Beijing Academy of Agriculture and Forestry Sciences Beijing Engineering Research Center for Edible Mushroom Ministry of Agriculture Beijing ChinaChina Agricultural University Beijing ChinaDepartment of Agricultural Biology Faculty of Agriculture University of Ruhuna Kamburupitiya Sri LankaKey Laboratory of Urban Agriculture (North) Institute of Plant Protection Beijing Academy of Agriculture and Forestry Sciences Beijing Engineering Research Center for Edible Mushroom Ministry of Agriculture Beijing ChinaAbstract Oudemansiella aparlosarca is an edible mushroom possessing medicinal and health benefits. Although there are studies on the cultivation of O. aparlosarca, only a few studies have focused on its genetics and life cycle. Therefore, the main objective of this study was to identify the nuclear conditions of basidiospores and homokaryotic and heterokaryotic hyphal cells and to determine the influence of different nuclear conditions on basidiospore diameter in O. aparlosarca. Two parental strains: strain‐55 and strain‐81 were used. Staining of basidiospores and hyphal cells in the apical region was performed. We observed the following nuclear conditions: non‐nucleate, mononucleate, binucleate, and multinucleate. In both parental strains, binucleate spores were predominant, while the number of non‐nucleate spores was the lowest. The diameter of non‐nucleate spores was the smallest, being 11.52 µm and 12.15 µm in parental strain‐81 and strain‐55, respectively, while multinucleate spores had the largest diameter, being 14.78 µm in both parental strains. Both homokaryotic and heterokaryotic strains were identified in isolated single spores from parental strains. Binucleate cells were majorly present in heterokaryotic hyphal cells, and multinucleate cells were predominant in homokaryotic hyphal cells. We conclude that O. aparlosarca contains homokaryotic and heterokaryotic basidiospores, which indicates an amphithallic life cycle. The observed binucleate spores might be the result of post‐meiotic mitosis.https://doi.org/10.1002/mbo3.1233amphithallic life cyclebinucleatemultinucleateOudemansiella aparlosarcapost‐meiotic mitosisspore size |
| spellingShingle | Roy Rebecca Qi Gao Yujin Cui Chengbo Rong Yu Liu Wensheng Zhao Wasantha Kumara Shouxian Wang Nuclear conditions of basidiospores and hyphal cells in the edible mushroom Oudemansiella aparlosarca MicrobiologyOpen amphithallic life cycle binucleate multinucleate Oudemansiella aparlosarca post‐meiotic mitosis spore size |
| title | Nuclear conditions of basidiospores and hyphal cells in the edible mushroom Oudemansiella aparlosarca |
| title_full | Nuclear conditions of basidiospores and hyphal cells in the edible mushroom Oudemansiella aparlosarca |
| title_fullStr | Nuclear conditions of basidiospores and hyphal cells in the edible mushroom Oudemansiella aparlosarca |
| title_full_unstemmed | Nuclear conditions of basidiospores and hyphal cells in the edible mushroom Oudemansiella aparlosarca |
| title_short | Nuclear conditions of basidiospores and hyphal cells in the edible mushroom Oudemansiella aparlosarca |
| title_sort | nuclear conditions of basidiospores and hyphal cells in the edible mushroom oudemansiella aparlosarca |
| topic | amphithallic life cycle binucleate multinucleate Oudemansiella aparlosarca post‐meiotic mitosis spore size |
| url | https://doi.org/10.1002/mbo3.1233 |
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