Biotin/avidin sandwich enzyme-linked immunosorbent assay for Culicidae mosquito blood meal identification
The knowledge of mosquitoes Culicidae host feeding patterns is basic to understand the roles of different species and to indicate their importance in the epidemiology of arthropod-borne diseases. A laboratory assay was developed aiming at standardizing the biotin-avidin sandwich enzyme-linked immuno...
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| Format: | Article |
| Language: | English |
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SciELO
2008-01-01
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| Series: | Journal of Venomous Animals and Toxins including Tropical Diseases |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200008 |
| _version_ | 1818481717503066112 |
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| author | A. M. Marassá M. D. B. Rosa A. C. Gomes C. A. Consales |
| author_facet | A. M. Marassá M. D. B. Rosa A. C. Gomes C. A. Consales |
| author_sort | A. M. Marassá |
| collection | DOAJ |
| description | The knowledge of mosquitoes Culicidae host feeding patterns is basic to understand the roles of different species and to indicate their importance in the epidemiology of arthropod-borne diseases. A laboratory assay was developed aiming at standardizing the biotin-avidin sandwich enzyme-linked immunosorbent assay, which was unprecedented for mosquito blood meal identification. The enzyme-linked immunosorbent assay (ELISA) activity was evaluated by the detection of titers on each sample of the 28 blood-fed Culex quinquefasciatus. In light of the high sensitivity that the technique permits, by means of small quantities of specific antibodies commercially provided and phosphatase substrate which reinforces additional dilutions, human and rat blood meals were readily identified in all laboratory-raised Culex quinquefasciatus tested. The assay was effective to detect human blood meal dilutions up to 1:4,096, which enables the technique to be applied in field studies. Additionally, the present results indicate a significant difference between the detection patterns recorded from human blood meal which corroborate the results of host feeding patterns. |
| first_indexed | 2024-12-10T11:38:49Z |
| format | Article |
| id | doaj.art-c65666ab0f614f8e962de0aefa831d6f |
| institution | Directory Open Access Journal |
| issn | 1678-9199 |
| language | English |
| last_indexed | 2024-12-10T11:38:49Z |
| publishDate | 2008-01-01 |
| publisher | SciELO |
| record_format | Article |
| series | Journal of Venomous Animals and Toxins including Tropical Diseases |
| spelling | doaj.art-c65666ab0f614f8e962de0aefa831d6f2022-12-22T01:50:20ZengSciELOJournal of Venomous Animals and Toxins including Tropical Diseases1678-91992008-01-0114230331210.1590/S1678-91992008000200008Biotin/avidin sandwich enzyme-linked immunosorbent assay for Culicidae mosquito blood meal identificationA. M. MarassáM. D. B. RosaA. C. GomesC. A. ConsalesThe knowledge of mosquitoes Culicidae host feeding patterns is basic to understand the roles of different species and to indicate their importance in the epidemiology of arthropod-borne diseases. A laboratory assay was developed aiming at standardizing the biotin-avidin sandwich enzyme-linked immunosorbent assay, which was unprecedented for mosquito blood meal identification. The enzyme-linked immunosorbent assay (ELISA) activity was evaluated by the detection of titers on each sample of the 28 blood-fed Culex quinquefasciatus. In light of the high sensitivity that the technique permits, by means of small quantities of specific antibodies commercially provided and phosphatase substrate which reinforces additional dilutions, human and rat blood meals were readily identified in all laboratory-raised Culex quinquefasciatus tested. The assay was effective to detect human blood meal dilutions up to 1:4,096, which enables the technique to be applied in field studies. Additionally, the present results indicate a significant difference between the detection patterns recorded from human blood meal which corroborate the results of host feeding patterns.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200008biotin/avidin sandwich enzyme-linked immunosorbent assayCulicidaeblood meal identification |
| spellingShingle | A. M. Marassá M. D. B. Rosa A. C. Gomes C. A. Consales Biotin/avidin sandwich enzyme-linked immunosorbent assay for Culicidae mosquito blood meal identification Journal of Venomous Animals and Toxins including Tropical Diseases biotin/avidin sandwich enzyme-linked immunosorbent assay Culicidae blood meal identification |
| title | Biotin/avidin sandwich enzyme-linked immunosorbent assay for Culicidae mosquito blood meal identification |
| title_full | Biotin/avidin sandwich enzyme-linked immunosorbent assay for Culicidae mosquito blood meal identification |
| title_fullStr | Biotin/avidin sandwich enzyme-linked immunosorbent assay for Culicidae mosquito blood meal identification |
| title_full_unstemmed | Biotin/avidin sandwich enzyme-linked immunosorbent assay for Culicidae mosquito blood meal identification |
| title_short | Biotin/avidin sandwich enzyme-linked immunosorbent assay for Culicidae mosquito blood meal identification |
| title_sort | biotin avidin sandwich enzyme linked immunosorbent assay for culicidae mosquito blood meal identification |
| topic | biotin/avidin sandwich enzyme-linked immunosorbent assay Culicidae blood meal identification |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000200008 |
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