Effects of US3 protein kinase activity on localization of UL31/UL34 protein and nucleocapsids egress of duck plague virus
ABSTRACT: Duck plague virus (DPV) is a pathogen causing duck plague and has caused huge economic losses in poultry industry. In our previous report, US3 gene deletion from DPV genome seriously impaired virus replication. In this study, we constructed a US3 kinase-inactive mutant (US3K213A) to furthe...
| Main Authors: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-03-01
|
| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S003257912200712X |
| _version_ | 1811162835038765056 |
|---|---|
| author | Liyao Deng Anchun Cheng Mingshu Wang Wei Zhang Bin Tian Ying Wu Qiao Yang Xumin Ou Sai Mao Di Sun Shaqiu Zhang Juan Huang Qun Gao Xinxin Zhao Renyong Jia Shun Chen Mafeng Liu Dekang Zhu |
| author_facet | Liyao Deng Anchun Cheng Mingshu Wang Wei Zhang Bin Tian Ying Wu Qiao Yang Xumin Ou Sai Mao Di Sun Shaqiu Zhang Juan Huang Qun Gao Xinxin Zhao Renyong Jia Shun Chen Mafeng Liu Dekang Zhu |
| author_sort | Liyao Deng |
| collection | DOAJ |
| description | ABSTRACT: Duck plague virus (DPV) is a pathogen causing duck plague and has caused huge economic losses in poultry industry. In our previous report, US3 gene deletion from DPV genome seriously impaired virus replication. In this study, we constructed a US3 kinase-inactive mutant (US3K213A) to further explore the function of US3 protein (pUS3) in DPV. Our results showed that the loss of pUS3 kinase activity caused lower viral titers, smaller plaque sizes and a blockage of capsids nuclear egress including primary enveloped virion (PEV) accumulation compared to the parental virus infection. It indicates that the effects of DPV pUS3 on viral propagation depended on its kinase activity. In addition, we conducted electron microscopy analysis to show the outer nuclear membrane (ONM) evaginations and the nuclear envelope (NE) deep invagination in US3K213A-infected cells. Finally, an irregular distribution of pUL31/pUL34 in the NE in △US3- and US3K213A-infected cells and an interaction of pUS3 and pUL31 were found, which suggests that pUS3 potentially targets pUL31 and regulates the localization of pUL31/pUL34 to promote nucleocapsids egress through its kinase activity. |
| first_indexed | 2024-04-10T06:35:33Z |
| format | Article |
| id | doaj.art-c658e4c0cc8b4279845f4023d103aa22 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-10T06:35:33Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-c658e4c0cc8b4279845f4023d103aa222023-03-01T04:30:15ZengElsevierPoultry Science0032-57912023-03-011023102418Effects of US3 protein kinase activity on localization of UL31/UL34 protein and nucleocapsids egress of duck plague virusLiyao Deng0Anchun Cheng1Mingshu Wang2Wei Zhang3Bin Tian4Ying Wu5Qiao Yang6Xumin Ou7Sai Mao8Di Sun9Shaqiu Zhang10Juan Huang11Qun Gao12Xinxin Zhao13Renyong Jia14Shun Chen15Mafeng Liu16Dekang Zhu17Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Corresponding author:Sinopharm Yangzhou VAC Biological Engineering CO., Ltd., Yangzhou City, Jingshu, 225100, PR ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR ChinaInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR ChinaKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan, 611130, PR ChinaABSTRACT: Duck plague virus (DPV) is a pathogen causing duck plague and has caused huge economic losses in poultry industry. In our previous report, US3 gene deletion from DPV genome seriously impaired virus replication. In this study, we constructed a US3 kinase-inactive mutant (US3K213A) to further explore the function of US3 protein (pUS3) in DPV. Our results showed that the loss of pUS3 kinase activity caused lower viral titers, smaller plaque sizes and a blockage of capsids nuclear egress including primary enveloped virion (PEV) accumulation compared to the parental virus infection. It indicates that the effects of DPV pUS3 on viral propagation depended on its kinase activity. In addition, we conducted electron microscopy analysis to show the outer nuclear membrane (ONM) evaginations and the nuclear envelope (NE) deep invagination in US3K213A-infected cells. Finally, an irregular distribution of pUL31/pUL34 in the NE in △US3- and US3K213A-infected cells and an interaction of pUS3 and pUL31 were found, which suggests that pUS3 potentially targets pUL31 and regulates the localization of pUL31/pUL34 to promote nucleocapsids egress through its kinase activity.http://www.sciencedirect.com/science/article/pii/S003257912200712Xduck plague virusUS3nucleocapsids egressUL31/UL34 |
| spellingShingle | Liyao Deng Anchun Cheng Mingshu Wang Wei Zhang Bin Tian Ying Wu Qiao Yang Xumin Ou Sai Mao Di Sun Shaqiu Zhang Juan Huang Qun Gao Xinxin Zhao Renyong Jia Shun Chen Mafeng Liu Dekang Zhu Effects of US3 protein kinase activity on localization of UL31/UL34 protein and nucleocapsids egress of duck plague virus Poultry Science duck plague virus US3 nucleocapsids egress UL31/UL34 |
| title | Effects of US3 protein kinase activity on localization of UL31/UL34 protein and nucleocapsids egress of duck plague virus |
| title_full | Effects of US3 protein kinase activity on localization of UL31/UL34 protein and nucleocapsids egress of duck plague virus |
| title_fullStr | Effects of US3 protein kinase activity on localization of UL31/UL34 protein and nucleocapsids egress of duck plague virus |
| title_full_unstemmed | Effects of US3 protein kinase activity on localization of UL31/UL34 protein and nucleocapsids egress of duck plague virus |
| title_short | Effects of US3 protein kinase activity on localization of UL31/UL34 protein and nucleocapsids egress of duck plague virus |
| title_sort | effects of us3 protein kinase activity on localization of ul31 ul34 protein and nucleocapsids egress of duck plague virus |
| topic | duck plague virus US3 nucleocapsids egress UL31/UL34 |
| url | http://www.sciencedirect.com/science/article/pii/S003257912200712X |
| work_keys_str_mv | AT liyaodeng effectsofus3proteinkinaseactivityonlocalizationoful31ul34proteinandnucleocapsidsegressofduckplaguevirus AT anchuncheng effectsofus3proteinkinaseactivityonlocalizationoful31ul34proteinandnucleocapsidsegressofduckplaguevirus AT mingshuwang effectsofus3proteinkinaseactivityonlocalizationoful31ul34proteinandnucleocapsidsegressofduckplaguevirus AT weizhang effectsofus3proteinkinaseactivityonlocalizationoful31ul34proteinandnucleocapsidsegressofduckplaguevirus AT bintian effectsofus3proteinkinaseactivityonlocalizationoful31ul34proteinandnucleocapsidsegressofduckplaguevirus AT yingwu effectsofus3proteinkinaseactivityonlocalizationoful31ul34proteinandnucleocapsidsegressofduckplaguevirus AT qiaoyang effectsofus3proteinkinaseactivityonlocalizationoful31ul34proteinandnucleocapsidsegressofduckplaguevirus AT xuminou effectsofus3proteinkinaseactivityonlocalizationoful31ul34proteinandnucleocapsidsegressofduckplaguevirus AT saimao effectsofus3proteinkinaseactivityonlocalizationoful31ul34proteinandnucleocapsidsegressofduckplaguevirus AT disun effectsofus3proteinkinaseactivityonlocalizationoful31ul34proteinandnucleocapsidsegressofduckplaguevirus AT shaqiuzhang effectsofus3proteinkinaseactivityonlocalizationoful31ul34proteinandnucleocapsidsegressofduckplaguevirus AT juanhuang effectsofus3proteinkinaseactivityonlocalizationoful31ul34proteinandnucleocapsidsegressofduckplaguevirus AT qungao effectsofus3proteinkinaseactivityonlocalizationoful31ul34proteinandnucleocapsidsegressofduckplaguevirus AT xinxinzhao effectsofus3proteinkinaseactivityonlocalizationoful31ul34proteinandnucleocapsidsegressofduckplaguevirus AT renyongjia effectsofus3proteinkinaseactivityonlocalizationoful31ul34proteinandnucleocapsidsegressofduckplaguevirus AT shunchen effectsofus3proteinkinaseactivityonlocalizationoful31ul34proteinandnucleocapsidsegressofduckplaguevirus AT mafengliu effectsofus3proteinkinaseactivityonlocalizationoful31ul34proteinandnucleocapsidsegressofduckplaguevirus AT dekangzhu effectsofus3proteinkinaseactivityonlocalizationoful31ul34proteinandnucleocapsidsegressofduckplaguevirus |