Cloning, expression and activity of ATP-binding protein in Bacillus thuringiensis toxicity modulation against Aedes aegypti
Abstract Background Bacillus thuringiensis israelensis (Bti) is a widely used mosquitocidal microbial pesticide due to its high toxicity. ATP-binding proteins (ABP) are prevalently detected in insects and are related to reaction against Bti toxins. However, the function of ABP in mosquito biocontrol...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-06-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-019-3560-2 |
| _version_ | 1819276763134427136 |
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| author | Guo-hui Zhao Jian-nan Liu Xiao-hua Hu Khadija Batool Liang Jin Chen-xu Wu Juan Wu Hong Chen Xiao-yan Jiang Zhao-hui Yang Xian-hui Huang En-jiong Huang Xiao-Qiang Yu Xiong Guan Ling-ling Zhang |
| author_facet | Guo-hui Zhao Jian-nan Liu Xiao-hua Hu Khadija Batool Liang Jin Chen-xu Wu Juan Wu Hong Chen Xiao-yan Jiang Zhao-hui Yang Xian-hui Huang En-jiong Huang Xiao-Qiang Yu Xiong Guan Ling-ling Zhang |
| author_sort | Guo-hui Zhao |
| collection | DOAJ |
| description | Abstract Background Bacillus thuringiensis israelensis (Bti) is a widely used mosquitocidal microbial pesticide due to its high toxicity. ATP-binding proteins (ABP) are prevalently detected in insects and are related to reaction against Bti toxins. However, the function of ABP in mosquito biocontrol is little known, especially in Aedes aegypti. Therefore, this study aimed to clarify the function of ABP in Ae. aegypti against Bti toxin. Results Aedes aegypti ABP (GenBank: XM_001661856.2) was cloned, expressed and purified in this study. Far-western blotting and ELISA were also carried out to confirm the interaction between ABP and Cry11Aa. A bioassay of Cry11Aa was performed both in the presence and absence of ABP, which showed that the mortality of Ae. aegypti is increased with an increase in ABP. Conclusions Our results suggest that ABP in Ae. aegypti can modulate the toxicity of Cry11Aa toxin to mosquitoes by binding to Bti toxin. This could not only enrich the mechanism of Bt toxin, but also provide more data for the biocontrol of this transmission vector. |
| first_indexed | 2024-12-23T23:45:23Z |
| format | Article |
| id | doaj.art-c65a162c6c814222ba358148c92ca94f |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-23T23:45:23Z |
| publishDate | 2019-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-c65a162c6c814222ba358148c92ca94f2022-12-21T17:25:31ZengBMCParasites & Vectors1756-33052019-06-011211910.1186/s13071-019-3560-2Cloning, expression and activity of ATP-binding protein in Bacillus thuringiensis toxicity modulation against Aedes aegyptiGuo-hui Zhao0Jian-nan Liu1Xiao-hua Hu2Khadija Batool3Liang Jin4Chen-xu Wu5Juan Wu6Hong Chen7Xiao-yan Jiang8Zhao-hui Yang9Xian-hui Huang10En-jiong Huang11Xiao-Qiang Yu12Xiong Guan13Ling-ling Zhang14State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & School of Life Science, Fujian Agriculture and Forestry UniversityState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & School of Life Science, Fujian Agriculture and Forestry UniversityState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & School of Life Science, Fujian Agriculture and Forestry UniversityState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & School of Life Science, Fujian Agriculture and Forestry UniversityState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & School of Life Science, Fujian Agriculture and Forestry UniversityState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & School of Life Science, Fujian Agriculture and Forestry UniversityState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & School of Life Science, Fujian Agriculture and Forestry UniversityState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & School of Life Science, Fujian Agriculture and Forestry UniversityState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & School of Life Science, Fujian Agriculture and Forestry UniversityState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & School of Life Science, Fujian Agriculture and Forestry UniversityState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & School of Life Science, Fujian Agriculture and Forestry UniversityFujian International Travel HealthCare CenterGuangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology & School of Life Sciences, South China Normal UniversityState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & School of Life Science, Fujian Agriculture and Forestry UniversityState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & School of Life Science, Fujian Agriculture and Forestry UniversityAbstract Background Bacillus thuringiensis israelensis (Bti) is a widely used mosquitocidal microbial pesticide due to its high toxicity. ATP-binding proteins (ABP) are prevalently detected in insects and are related to reaction against Bti toxins. However, the function of ABP in mosquito biocontrol is little known, especially in Aedes aegypti. Therefore, this study aimed to clarify the function of ABP in Ae. aegypti against Bti toxin. Results Aedes aegypti ABP (GenBank: XM_001661856.2) was cloned, expressed and purified in this study. Far-western blotting and ELISA were also carried out to confirm the interaction between ABP and Cry11Aa. A bioassay of Cry11Aa was performed both in the presence and absence of ABP, which showed that the mortality of Ae. aegypti is increased with an increase in ABP. Conclusions Our results suggest that ABP in Ae. aegypti can modulate the toxicity of Cry11Aa toxin to mosquitoes by binding to Bti toxin. This could not only enrich the mechanism of Bt toxin, but also provide more data for the biocontrol of this transmission vector.http://link.springer.com/article/10.1186/s13071-019-3560-2Aedes aegyptiATP-binding proteinBiocontrolBtiInteraction |
| spellingShingle | Guo-hui Zhao Jian-nan Liu Xiao-hua Hu Khadija Batool Liang Jin Chen-xu Wu Juan Wu Hong Chen Xiao-yan Jiang Zhao-hui Yang Xian-hui Huang En-jiong Huang Xiao-Qiang Yu Xiong Guan Ling-ling Zhang Cloning, expression and activity of ATP-binding protein in Bacillus thuringiensis toxicity modulation against Aedes aegypti Parasites & Vectors Aedes aegypti ATP-binding protein Biocontrol Bti Interaction |
| title | Cloning, expression and activity of ATP-binding protein in Bacillus thuringiensis toxicity modulation against Aedes aegypti |
| title_full | Cloning, expression and activity of ATP-binding protein in Bacillus thuringiensis toxicity modulation against Aedes aegypti |
| title_fullStr | Cloning, expression and activity of ATP-binding protein in Bacillus thuringiensis toxicity modulation against Aedes aegypti |
| title_full_unstemmed | Cloning, expression and activity of ATP-binding protein in Bacillus thuringiensis toxicity modulation against Aedes aegypti |
| title_short | Cloning, expression and activity of ATP-binding protein in Bacillus thuringiensis toxicity modulation against Aedes aegypti |
| title_sort | cloning expression and activity of atp binding protein in bacillus thuringiensis toxicity modulation against aedes aegypti |
| topic | Aedes aegypti ATP-binding protein Biocontrol Bti Interaction |
| url | http://link.springer.com/article/10.1186/s13071-019-3560-2 |
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