Biomimetic-Functionalized, Tannic Acid-Templated Mesoporous Silica as a New Support for Immobilization of NHase
Tannic acid-templated mesoporous silica (TAMS) was synthesized using a simple nonsurfactant template method and dopamine-functionalized TAMS (Dop-TAMS), which was prepared via a biomimetic coating, was developed as a new support for immobilization of NHase (NHase@Dop-TAMS). The Dop-TAMS was thorough...
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MDPI AG
2017-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/22/10/1597 |
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| author | Jun-kai Gao Zi-jun Zhang Yan-jun Jiang Yan Chen Shu-feng Gao |
| author_facet | Jun-kai Gao Zi-jun Zhang Yan-jun Jiang Yan Chen Shu-feng Gao |
| author_sort | Jun-kai Gao |
| collection | DOAJ |
| description | Tannic acid-templated mesoporous silica (TAMS) was synthesized using a simple nonsurfactant template method and dopamine-functionalized TAMS (Dop-TAMS), which was prepared via a biomimetic coating, was developed as a new support for immobilization of NHase (NHase@Dop-TAMS). The Dop-TAMS was thoroughly characterized by the transmission electron microscopy (TEM), scanning electron microscopy (SEM), Brunauer–Emmett–Teller (BET), and Fourier transform infrared (FT-IR) and the results showed that the Dop-TAMS possessed sufficiently large pore size and volume for the accommodation of NHase. Studying the thermal stability, storage, shaking stability, and pH stability of the free and immobilized NHase indicated that the catalytic properties of NHase@Dop-TAMS were significantly enhanced. Moreover, the NHase@Dop-TAMS exhibited good reusability. All the results demonstrated that Dop-TAMS could be used as an excellent matrix for the immobilization of NHase. |
| first_indexed | 2024-12-23T21:11:17Z |
| format | Article |
| id | doaj.art-c65a4e9a9ff946eab4e3fc8e46821d0a |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-23T21:11:17Z |
| publishDate | 2017-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c65a4e9a9ff946eab4e3fc8e46821d0a2022-12-21T17:31:03ZengMDPI AGMolecules1420-30492017-09-012210159710.3390/molecules22101597molecules22101597Biomimetic-Functionalized, Tannic Acid-Templated Mesoporous Silica as a New Support for Immobilization of NHaseJun-kai Gao0Zi-jun Zhang1Yan-jun Jiang2Yan Chen3Shu-feng Gao4School of Port and Transportation Engineering, Zhejiang Ocean University, Zhoushan 316022, ChinaSchool of Port and Transportation Engineering, Zhejiang Ocean University, Zhoushan 316022, ChinaSchool of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, ChinaSchool of Port and Transportation Engineering, Zhejiang Ocean University, Zhoushan 316022, ChinaYinzhouKefeng New Material of Polymer Co. Ltd., Ningbo 315100, ChinaTannic acid-templated mesoporous silica (TAMS) was synthesized using a simple nonsurfactant template method and dopamine-functionalized TAMS (Dop-TAMS), which was prepared via a biomimetic coating, was developed as a new support for immobilization of NHase (NHase@Dop-TAMS). The Dop-TAMS was thoroughly characterized by the transmission electron microscopy (TEM), scanning electron microscopy (SEM), Brunauer–Emmett–Teller (BET), and Fourier transform infrared (FT-IR) and the results showed that the Dop-TAMS possessed sufficiently large pore size and volume for the accommodation of NHase. Studying the thermal stability, storage, shaking stability, and pH stability of the free and immobilized NHase indicated that the catalytic properties of NHase@Dop-TAMS were significantly enhanced. Moreover, the NHase@Dop-TAMS exhibited good reusability. All the results demonstrated that Dop-TAMS could be used as an excellent matrix for the immobilization of NHase.https://www.mdpi.com/1420-3049/22/10/1597dopaminemesoporous silicatannic acidimmobilizationnitrile hydratase |
| spellingShingle | Jun-kai Gao Zi-jun Zhang Yan-jun Jiang Yan Chen Shu-feng Gao Biomimetic-Functionalized, Tannic Acid-Templated Mesoporous Silica as a New Support for Immobilization of NHase Molecules dopamine mesoporous silica tannic acid immobilization nitrile hydratase |
| title | Biomimetic-Functionalized, Tannic Acid-Templated Mesoporous Silica as a New Support for Immobilization of NHase |
| title_full | Biomimetic-Functionalized, Tannic Acid-Templated Mesoporous Silica as a New Support for Immobilization of NHase |
| title_fullStr | Biomimetic-Functionalized, Tannic Acid-Templated Mesoporous Silica as a New Support for Immobilization of NHase |
| title_full_unstemmed | Biomimetic-Functionalized, Tannic Acid-Templated Mesoporous Silica as a New Support for Immobilization of NHase |
| title_short | Biomimetic-Functionalized, Tannic Acid-Templated Mesoporous Silica as a New Support for Immobilization of NHase |
| title_sort | biomimetic functionalized tannic acid templated mesoporous silica as a new support for immobilization of nhase |
| topic | dopamine mesoporous silica tannic acid immobilization nitrile hydratase |
| url | https://www.mdpi.com/1420-3049/22/10/1597 |
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