Effect of Rhizopus oryzae Fermentation on Kenaf-Based Polylactic Acid’s Monomer
Kenaf biomass is the potential as raw materials used to produce polylactic acid's monomer which is lactic acid via fermentation by Rhizopus oryzae. Kenaf biomass' structure is complex due to its lignin and cellulose content. This matter had encouraged it to undergo pre- treatment process a...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
IIUM Press, International Islamic University Malaysia
2011-12-01
|
| Series: | International Islamic University Malaysia Engineering Journal |
| Online Access: | http://journals.iium.edu.my/ejournal/index.php/iiumej/article/view/209 |
| _version_ | 1828888355616587776 |
|---|---|
| author | Nur Aimi Mohd Nasir Mohd Adlan Mustafa Kamalbahrin Nurhafizah Mohamad Hazleen Anuar Maizirwan Mel and Rashidi Othman |
| author_facet | Nur Aimi Mohd Nasir Mohd Adlan Mustafa Kamalbahrin Nurhafizah Mohamad Hazleen Anuar Maizirwan Mel and Rashidi Othman |
| author_sort | Nur Aimi Mohd Nasir |
| collection | DOAJ |
| description | Kenaf biomass is the potential as raw materials used to produce polylactic acid's monomer which is lactic acid via fermentation by Rhizopus oryzae. Kenaf biomass' structure is complex due to its lignin and cellulose content. This matter had encouraged it to undergo pre- treatment process as the initial step before fermentation process can be done. In this paper, kenaf biomass was treated with dilute sulphuric acid (H2SO4) to hydrolyze the cellulose content in it as well as to convert the cellulose into glucose- a carbon source for Rhizopus to grow. Then, the fermentation process was carried out in shake flask for 3 days at pH 6. Several conditions for fermentation process had been chosen which were 25oC at 150 rpm, 25 oC at 200 rpm, 37 oC at 150 rpm and 37oC at 200 rpm. In this fermentation process, 0.471 g/L, 0.428 g/L, 0.444 g/L and 0.38 g/L of lactic acid was produced respectively. Sample at 25oC at 200 rpm produced maximum amount of lactic acid compared to others.
ABSTRAK: Biojisim kenaf berpotensi sebagai bahan mentah dalam penghasilan monomer asid polylactic (poliester alifatik termoplastik diterbitkan daripada sumber boleh diperbaharu seperti kanji jagung) yang merupakan asid laktik menerusi penapaian oleh Rhizopus oryzae (sejenis fungus yang hidup dalam jirim organik yang telah mati). Struktur biojisim kenaf adalah kompleks disebabkan kandungan lignin dan selulosanya. Hal ini menyebabkan ia perlu melalui proses pra-rawatan sebagai langkah awal sebelum proses penapaian dijalankan. Dalam kertas ini, biojirim kenaf dirawat dengan asid sulfurik (H2SO4) yang dicairkan untuk menghidrolisis kandungan selulosa di dalamnya di samping menukar selulosa menjadi glukosa - sumber karbon bagi tumbesaran Rhizopus. Kemudian, proses penapaian dijalankan di dalam kelalang goncang selama 3 hari pada pH 6. Beberapa ciri proses penapaian telah dipilih iaitu 25 oC pada 150 rpm, 25 oC pada 200 rpm, 37 oC pada 150 rpm dan 37 oC pada 200 rpm. Dalam proses penapaian ini, 0.471 g/L, 0.428 g/L, 0.444 g/L dan 0.38 g/L asid laktik dihasilkan secara berturut. Sampel pada 25oC pada 200 rpm menghasilkan kadar asid laktik yang maksimum dibandingkan dengan yang lainnya.
KEY WORDS :Kenaf biomass, pre-treatment, lactic acid, Rhizopus oryzae, fermentation |
| first_indexed | 2024-12-13T12:21:11Z |
| format | Article |
| id | doaj.art-c65a5e1e759c43278f44eb9965f08af8 |
| institution | Directory Open Access Journal |
| issn | 1511-788X 2289-7860 |
| language | English |
| last_indexed | 2024-12-13T12:21:11Z |
| publishDate | 2011-12-01 |
| publisher | IIUM Press, International Islamic University Malaysia |
| record_format | Article |
| series | International Islamic University Malaysia Engineering Journal |
| spelling | doaj.art-c65a5e1e759c43278f44eb9965f08af82022-12-21T23:46:34ZengIIUM Press, International Islamic University MalaysiaInternational Islamic University Malaysia Engineering Journal1511-788X2289-78602011-12-0112410.31436/iiumej.v12i4.209Effect of Rhizopus oryzae Fermentation on Kenaf-Based Polylactic Acid’s MonomerNur Aimi Mohd Nasir0Mohd Adlan Mustafa KamalbahrinNurhafizah MohamadHazleen AnuarMaizirwan Meland Rashidi OthmanDepartment of Manufacturing and Materials Engineering, Faculty of Engineering, International Islamic University MalaysiaKenaf biomass is the potential as raw materials used to produce polylactic acid's monomer which is lactic acid via fermentation by Rhizopus oryzae. Kenaf biomass' structure is complex due to its lignin and cellulose content. This matter had encouraged it to undergo pre- treatment process as the initial step before fermentation process can be done. In this paper, kenaf biomass was treated with dilute sulphuric acid (H2SO4) to hydrolyze the cellulose content in it as well as to convert the cellulose into glucose- a carbon source for Rhizopus to grow. Then, the fermentation process was carried out in shake flask for 3 days at pH 6. Several conditions for fermentation process had been chosen which were 25oC at 150 rpm, 25 oC at 200 rpm, 37 oC at 150 rpm and 37oC at 200 rpm. In this fermentation process, 0.471 g/L, 0.428 g/L, 0.444 g/L and 0.38 g/L of lactic acid was produced respectively. Sample at 25oC at 200 rpm produced maximum amount of lactic acid compared to others. ABSTRAK: Biojisim kenaf berpotensi sebagai bahan mentah dalam penghasilan monomer asid polylactic (poliester alifatik termoplastik diterbitkan daripada sumber boleh diperbaharu seperti kanji jagung) yang merupakan asid laktik menerusi penapaian oleh Rhizopus oryzae (sejenis fungus yang hidup dalam jirim organik yang telah mati). Struktur biojisim kenaf adalah kompleks disebabkan kandungan lignin dan selulosanya. Hal ini menyebabkan ia perlu melalui proses pra-rawatan sebagai langkah awal sebelum proses penapaian dijalankan. Dalam kertas ini, biojirim kenaf dirawat dengan asid sulfurik (H2SO4) yang dicairkan untuk menghidrolisis kandungan selulosa di dalamnya di samping menukar selulosa menjadi glukosa - sumber karbon bagi tumbesaran Rhizopus. Kemudian, proses penapaian dijalankan di dalam kelalang goncang selama 3 hari pada pH 6. Beberapa ciri proses penapaian telah dipilih iaitu 25 oC pada 150 rpm, 25 oC pada 200 rpm, 37 oC pada 150 rpm dan 37 oC pada 200 rpm. Dalam proses penapaian ini, 0.471 g/L, 0.428 g/L, 0.444 g/L dan 0.38 g/L asid laktik dihasilkan secara berturut. Sampel pada 25oC pada 200 rpm menghasilkan kadar asid laktik yang maksimum dibandingkan dengan yang lainnya. KEY WORDS :Kenaf biomass, pre-treatment, lactic acid, Rhizopus oryzae, fermentationhttp://journals.iium.edu.my/ejournal/index.php/iiumej/article/view/209 |
| spellingShingle | Nur Aimi Mohd Nasir Mohd Adlan Mustafa Kamalbahrin Nurhafizah Mohamad Hazleen Anuar Maizirwan Mel and Rashidi Othman Effect of Rhizopus oryzae Fermentation on Kenaf-Based Polylactic Acid’s Monomer International Islamic University Malaysia Engineering Journal |
| title | Effect of Rhizopus oryzae Fermentation on Kenaf-Based Polylactic Acid’s Monomer |
| title_full | Effect of Rhizopus oryzae Fermentation on Kenaf-Based Polylactic Acid’s Monomer |
| title_fullStr | Effect of Rhizopus oryzae Fermentation on Kenaf-Based Polylactic Acid’s Monomer |
| title_full_unstemmed | Effect of Rhizopus oryzae Fermentation on Kenaf-Based Polylactic Acid’s Monomer |
| title_short | Effect of Rhizopus oryzae Fermentation on Kenaf-Based Polylactic Acid’s Monomer |
| title_sort | effect of rhizopus oryzae fermentation on kenaf based polylactic acid s monomer |
| url | http://journals.iium.edu.my/ejournal/index.php/iiumej/article/view/209 |
| work_keys_str_mv | AT nuraimimohdnasir effectofrhizopusoryzaefermentationonkenafbasedpolylacticacidsmonomer AT mohdadlanmustafakamalbahrin effectofrhizopusoryzaefermentationonkenafbasedpolylacticacidsmonomer AT nurhafizahmohamad effectofrhizopusoryzaefermentationonkenafbasedpolylacticacidsmonomer AT hazleenanuar effectofrhizopusoryzaefermentationonkenafbasedpolylacticacidsmonomer AT maizirwanmel effectofrhizopusoryzaefermentationonkenafbasedpolylacticacidsmonomer AT andrashidiothman effectofrhizopusoryzaefermentationonkenafbasedpolylacticacidsmonomer |