First case of canine visceral leishmaniasis in the midwestern of Santa Catarina State, Brazil
Abstract Canine visceral leishmaniasis (CVL) caused by Leishmania (Leishmania) infantum is transmitted by phlebotomine sandflies and a major zoonotic disease in Brazil. Due to the southward expansion of the disease within the country and the central role of dogs as urban reservoirs of the parasite,...
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| Format: | Article |
| Language: | English |
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Instituto Internacional de Ecologia
2021-06-01
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| Series: | Brazilian Journal of Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100208&tlng=en |
| _version_ | 1818979108126720000 |
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| author | A. O. Pinto D. Carvalho C. Frizzo K. Lopes G. B. Tessari T. Catecati L. C. Dhom-Lemos A. K. S. Pasquali P. F. Quaresma P. H. Stoco E. C. Grisard M. Steindel G. Wagner |
| author_facet | A. O. Pinto D. Carvalho C. Frizzo K. Lopes G. B. Tessari T. Catecati L. C. Dhom-Lemos A. K. S. Pasquali P. F. Quaresma P. H. Stoco E. C. Grisard M. Steindel G. Wagner |
| author_sort | A. O. Pinto |
| collection | DOAJ |
| description | Abstract Canine visceral leishmaniasis (CVL) caused by Leishmania (Leishmania) infantum is transmitted by phlebotomine sandflies and a major zoonotic disease in Brazil. Due to the southward expansion of the disease within the country and the central role of dogs as urban reservoirs of the parasite, we have investigated the occurrence of CVL in two municipalities Erval Velho and Herval d’Oeste in the Midwest region of Santa Catarina state. Peripheral blood samples from 126 dogs were collected in both cities and tested for anti-L. infantum antibodies by indirect enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence reaction (IIF) and for the presence of parasite DNA by polymerase chain reaction (PCR) in peripheral blood. From examined dogs, 35.71% (45/126) were positive for at least one of the three tests and two (1.6%) were positive in all performed tests. Twelve dogs (9.5%) were positive for both ELISA and IIF, while 21 dogs were exclusively positive for ELISA (16.7%), and 15 (11.9%) for IIF. L. infantum k-DNA was detected by PCR in 9 out of 126 dogs (7.1%) and clinical symptoms compatible with CVL were observed for 6 dogs. Taken together, these results indicate the transmission of CVL in this region, highlighting the needs for epidemiological surveillance and implementation of control measures for CVL transmission in this region. |
| first_indexed | 2024-12-20T16:54:17Z |
| format | Article |
| id | doaj.art-c65adba9110a4a53a70780ec9d83f0f4 |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-12-20T16:54:17Z |
| publishDate | 2021-06-01 |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-c65adba9110a4a53a70780ec9d83f0f42022-12-21T19:32:46ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43752021-06-018210.1590/1519-6984.241162First case of canine visceral leishmaniasis in the midwestern of Santa Catarina State, BrazilA. O. Pintohttps://orcid.org/0000-0001-9006-4822D. Carvalhohttps://orcid.org/0000-0002-0059-4350C. Frizzohttps://orcid.org/0000-0001-6434-1312K. Lopeshttps://orcid.org/0000-0002-1217-8805G. B. Tessarihttps://orcid.org/0000-0002-2859-837XT. Catecatihttps://orcid.org/0000-0001-9722-3021L. C. Dhom-Lemoshttps://orcid.org/0000-0002-5162-0045A. K. S. Pasqualihttps://orcid.org/0000-0003-0426-3839P. F. Quaresmahttps://orcid.org/0000-0001-8274-4166P. H. Stocohttps://orcid.org/0000-0003-0879-6173E. C. Grisardhttps://orcid.org/0000-0001-8916-8296M. Steindelhttps://orcid.org/0000-0002-2768-7469G. Wagnerhttps://orcid.org/0000-0001-5003-6595Abstract Canine visceral leishmaniasis (CVL) caused by Leishmania (Leishmania) infantum is transmitted by phlebotomine sandflies and a major zoonotic disease in Brazil. Due to the southward expansion of the disease within the country and the central role of dogs as urban reservoirs of the parasite, we have investigated the occurrence of CVL in two municipalities Erval Velho and Herval d’Oeste in the Midwest region of Santa Catarina state. Peripheral blood samples from 126 dogs were collected in both cities and tested for anti-L. infantum antibodies by indirect enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence reaction (IIF) and for the presence of parasite DNA by polymerase chain reaction (PCR) in peripheral blood. From examined dogs, 35.71% (45/126) were positive for at least one of the three tests and two (1.6%) were positive in all performed tests. Twelve dogs (9.5%) were positive for both ELISA and IIF, while 21 dogs were exclusively positive for ELISA (16.7%), and 15 (11.9%) for IIF. L. infantum k-DNA was detected by PCR in 9 out of 126 dogs (7.1%) and clinical symptoms compatible with CVL were observed for 6 dogs. Taken together, these results indicate the transmission of CVL in this region, highlighting the needs for epidemiological surveillance and implementation of control measures for CVL transmission in this region.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100208&tlng=envisceral leishmaniasispublic healthdogzoonosis |
| spellingShingle | A. O. Pinto D. Carvalho C. Frizzo K. Lopes G. B. Tessari T. Catecati L. C. Dhom-Lemos A. K. S. Pasquali P. F. Quaresma P. H. Stoco E. C. Grisard M. Steindel G. Wagner First case of canine visceral leishmaniasis in the midwestern of Santa Catarina State, Brazil Brazilian Journal of Biology visceral leishmaniasis public health dog zoonosis |
| title | First case of canine visceral leishmaniasis in the midwestern of Santa Catarina State, Brazil |
| title_full | First case of canine visceral leishmaniasis in the midwestern of Santa Catarina State, Brazil |
| title_fullStr | First case of canine visceral leishmaniasis in the midwestern of Santa Catarina State, Brazil |
| title_full_unstemmed | First case of canine visceral leishmaniasis in the midwestern of Santa Catarina State, Brazil |
| title_short | First case of canine visceral leishmaniasis in the midwestern of Santa Catarina State, Brazil |
| title_sort | first case of canine visceral leishmaniasis in the midwestern of santa catarina state brazil |
| topic | visceral leishmaniasis public health dog zoonosis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842022000100208&tlng=en |
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