The relationship between the intensity of Gasterophilus intestinalis larvae infection and the serum and salivary humoral immune response in horses
Abstract Infection with Gasterophilus intestinalis (botfly) larvae often occurs in horses. The aim of the study was to isolate the larvae of G. intestinalis and evaluate the serum and salivary humoral immune response using self-developed ELISA in G. intestinalis infected horses. Blood serum or saliv...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-21482-z |
| _version_ | 1811336350019878912 |
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| author | Monika Pawlas-Opiela Paulina Jawor Józef Galli Agnieszka Zak-Bochenek Michał Gorczykowski Joanna Galli Zenon Sołtysiak Tadeusz Stefaniak |
| author_facet | Monika Pawlas-Opiela Paulina Jawor Józef Galli Agnieszka Zak-Bochenek Michał Gorczykowski Joanna Galli Zenon Sołtysiak Tadeusz Stefaniak |
| author_sort | Monika Pawlas-Opiela |
| collection | DOAJ |
| description | Abstract Infection with Gasterophilus intestinalis (botfly) larvae often occurs in horses. The aim of the study was to isolate the larvae of G. intestinalis and evaluate the serum and salivary humoral immune response using self-developed ELISA in G. intestinalis infected horses. Blood serum or saliva samples were taken from 125 infected horses and 54 uninfected slaughtered horses. The antigens from G. intestinalis larvae were used for development of ELISA in order to evaluate the intensity of G. intestinalis IgG, IgM, and IgA antibody reactivity in the serum or saliva of naturally infected horses and horses without larvae in the gastrointestinal tract (control group). Serum antibodies against second and third larvae’s stadium antigens reacted significantly more intensively in infected than in healthy horses in IgG (p ≤ 0.001; p ≤ 0.05, respectively) and IgA (p ≤ 0.05;p ≤ 0.001, respectively) classes. Salivary IgG and IgA specific’s antibody reactivity was significantly higher in horses with moderate (p ≤ 0.01) and severe infection (p ≤ 0.001) compared to the healthy horses. The determination of the G. intestinalis IgG and IgA antibody activity in saliva and serum may be used for detecting horses moderately and severely infested with larvae. |
| first_indexed | 2024-04-13T17:38:09Z |
| format | Article |
| id | doaj.art-c65b2a934bb24d72ad8db0cef8fd38e0 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T17:38:09Z |
| publishDate | 2022-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c65b2a934bb24d72ad8db0cef8fd38e02022-12-22T02:37:17ZengNature PortfolioScientific Reports2045-23222022-10-011211710.1038/s41598-022-21482-zThe relationship between the intensity of Gasterophilus intestinalis larvae infection and the serum and salivary humoral immune response in horsesMonika Pawlas-Opiela0Paulina Jawor1Józef Galli2Agnieszka Zak-Bochenek3Michał Gorczykowski4Joanna Galli5Zenon Sołtysiak6Tadeusz Stefaniak7Department of Immunology, Pathophysiology and Veterinary Preventive, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life SciencesDepartment of Immunology, Pathophysiology and Veterinary Preventive, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life SciencesDepartment of Immunology, Pathophysiology and Veterinary Preventive, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life SciencesDepartment of Immunology, Pathophysiology and Veterinary Preventive, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life SciencesDepartment of Internal Diseases and Clinic of Diseases of Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life SciencesDepartment of Immunology, Pathophysiology and Veterinary Preventive, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life SciencesDepartment of Internal Diseases and Clinic of Diseases of Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life SciencesDepartment of Immunology, Pathophysiology and Veterinary Preventive, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life SciencesAbstract Infection with Gasterophilus intestinalis (botfly) larvae often occurs in horses. The aim of the study was to isolate the larvae of G. intestinalis and evaluate the serum and salivary humoral immune response using self-developed ELISA in G. intestinalis infected horses. Blood serum or saliva samples were taken from 125 infected horses and 54 uninfected slaughtered horses. The antigens from G. intestinalis larvae were used for development of ELISA in order to evaluate the intensity of G. intestinalis IgG, IgM, and IgA antibody reactivity in the serum or saliva of naturally infected horses and horses without larvae in the gastrointestinal tract (control group). Serum antibodies against second and third larvae’s stadium antigens reacted significantly more intensively in infected than in healthy horses in IgG (p ≤ 0.001; p ≤ 0.05, respectively) and IgA (p ≤ 0.05;p ≤ 0.001, respectively) classes. Salivary IgG and IgA specific’s antibody reactivity was significantly higher in horses with moderate (p ≤ 0.01) and severe infection (p ≤ 0.001) compared to the healthy horses. The determination of the G. intestinalis IgG and IgA antibody activity in saliva and serum may be used for detecting horses moderately and severely infested with larvae.https://doi.org/10.1038/s41598-022-21482-z |
| spellingShingle | Monika Pawlas-Opiela Paulina Jawor Józef Galli Agnieszka Zak-Bochenek Michał Gorczykowski Joanna Galli Zenon Sołtysiak Tadeusz Stefaniak The relationship between the intensity of Gasterophilus intestinalis larvae infection and the serum and salivary humoral immune response in horses Scientific Reports |
| title | The relationship between the intensity of Gasterophilus intestinalis larvae infection and the serum and salivary humoral immune response in horses |
| title_full | The relationship between the intensity of Gasterophilus intestinalis larvae infection and the serum and salivary humoral immune response in horses |
| title_fullStr | The relationship between the intensity of Gasterophilus intestinalis larvae infection and the serum and salivary humoral immune response in horses |
| title_full_unstemmed | The relationship between the intensity of Gasterophilus intestinalis larvae infection and the serum and salivary humoral immune response in horses |
| title_short | The relationship between the intensity of Gasterophilus intestinalis larvae infection and the serum and salivary humoral immune response in horses |
| title_sort | relationship between the intensity of gasterophilus intestinalis larvae infection and the serum and salivary humoral immune response in horses |
| url | https://doi.org/10.1038/s41598-022-21482-z |
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