Effect of Alpha-Lipoic Acid on Rat Ventricles and Atria under LPS-Induced Oxidative Stress
Alpha-lipoic acid (α-LA) is a disulfide compound and one of the most effective antioxidants. Many studies have indicated positive effects of α-LA in the prevention of pathologic conditions mediated by oxidative stress, such as cardiovascular diseases. However, the therapeutic potential of α-LA for t...
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MDPI AG
2022-04-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/4/734 |
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| author | Beata Skibska Anna Goraca Agnieszka Skibska Andrzej Stanczak |
| author_facet | Beata Skibska Anna Goraca Agnieszka Skibska Andrzej Stanczak |
| author_sort | Beata Skibska |
| collection | DOAJ |
| description | Alpha-lipoic acid (α-LA) is a disulfide compound and one of the most effective antioxidants. Many studies have indicated positive effects of α-LA in the prevention of pathologic conditions mediated by oxidative stress, such as cardiovascular diseases. However, the therapeutic potential of α-LA for the heart has not been explored with regards to the ventricles and atria. The aim of our study was to evaluate the effects of α-LA on oxidative stress parameters and inflammation in the ventricles and atria of the heart in rats under LPS-induced oxidative stress. Wistar rats were divided into 4 groups: I—control (received 2 doses of 0.2 mL of 0.9% NaCl i.v., 0.5 h apart); II—α-LA (received 0.2 mL of 0.9% NaCl and 0.5 h later received α-LA 60 mg/kg b.w. i.v.); III—lipopolysaccharide (LPS) (received 0.2 mL of 0.9% NaCl and 0.5 h later received LPS 30 mg/kg b.w. i.v.); and IV—LPS + LA (received LPS 30 mg/kg b.w. i.v. and 0.5 h later received α-LA 60 mg/kg b.w. i.v.). Five hours later, the rats were euthanized. The hearts were surgically removed and weighed to estimate heart edema. The ventricular and atrium tissue was isolated to measure levels of TNF-α, IL-6, superoxide dismutase (SOD), thiobarbituric acid reactive substances (TBARS), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), total sulfhydryl groups (-SH), total glutathione (tGSH), reduced glutathione (GSH), glutathione disulfide (GSSG), and the GSH/GSSG ratio. LPS significantly increased TNF-α, IL-6, TBARS, and H<sub>2</sub>O<sub>2</sub> levels and decreased SOD, -SH groups, tGSH, the GSH/GSSG ratio, and GSH levels in rat ventricles and atria while α-LA administered after the injection of LPS significantly decreased TNF-α, IL-6, TBARS, and H<sub>2</sub>O<sub>2</sub> levels. α-LA also increased SOD and -SH group levels and ameliorated the glutathione redox status when compared to the LPS group. Our data suggest that α-LA administration 30 min after LPS infusion may effectively prevent inflammation and oxidative stress in the ventricles and atria. |
| first_indexed | 2024-03-09T11:14:19Z |
| format | Article |
| id | doaj.art-c65b2ecbb0ab44c0b30869d4bfadd722 |
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| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T11:14:19Z |
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| spelling | doaj.art-c65b2ecbb0ab44c0b30869d4bfadd7222023-12-01T00:36:00ZengMDPI AGAntioxidants2076-39212022-04-0111473410.3390/antiox11040734Effect of Alpha-Lipoic Acid on Rat Ventricles and Atria under LPS-Induced Oxidative StressBeata Skibska0Anna Goraca1Agnieszka Skibska2Andrzej Stanczak3Department of Applied Pharmacy, Faculty of Pharmacy, Medical University of Lodz, 90-151 Lodz, PolandSchool of Cosmetology and Health Sciences, 94-016 Lodz, PolandDepartment of Biomolecular Chemistry, Medical University of Lodz, 92-215 Lodz, PolandDepartment of Applied Pharmacy, Faculty of Pharmacy, Medical University of Lodz, 90-151 Lodz, PolandAlpha-lipoic acid (α-LA) is a disulfide compound and one of the most effective antioxidants. Many studies have indicated positive effects of α-LA in the prevention of pathologic conditions mediated by oxidative stress, such as cardiovascular diseases. However, the therapeutic potential of α-LA for the heart has not been explored with regards to the ventricles and atria. The aim of our study was to evaluate the effects of α-LA on oxidative stress parameters and inflammation in the ventricles and atria of the heart in rats under LPS-induced oxidative stress. Wistar rats were divided into 4 groups: I—control (received 2 doses of 0.2 mL of 0.9% NaCl i.v., 0.5 h apart); II—α-LA (received 0.2 mL of 0.9% NaCl and 0.5 h later received α-LA 60 mg/kg b.w. i.v.); III—lipopolysaccharide (LPS) (received 0.2 mL of 0.9% NaCl and 0.5 h later received LPS 30 mg/kg b.w. i.v.); and IV—LPS + LA (received LPS 30 mg/kg b.w. i.v. and 0.5 h later received α-LA 60 mg/kg b.w. i.v.). Five hours later, the rats were euthanized. The hearts were surgically removed and weighed to estimate heart edema. The ventricular and atrium tissue was isolated to measure levels of TNF-α, IL-6, superoxide dismutase (SOD), thiobarbituric acid reactive substances (TBARS), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), total sulfhydryl groups (-SH), total glutathione (tGSH), reduced glutathione (GSH), glutathione disulfide (GSSG), and the GSH/GSSG ratio. LPS significantly increased TNF-α, IL-6, TBARS, and H<sub>2</sub>O<sub>2</sub> levels and decreased SOD, -SH groups, tGSH, the GSH/GSSG ratio, and GSH levels in rat ventricles and atria while α-LA administered after the injection of LPS significantly decreased TNF-α, IL-6, TBARS, and H<sub>2</sub>O<sub>2</sub> levels. α-LA also increased SOD and -SH group levels and ameliorated the glutathione redox status when compared to the LPS group. Our data suggest that α-LA administration 30 min after LPS infusion may effectively prevent inflammation and oxidative stress in the ventricles and atria.https://www.mdpi.com/2076-3921/11/4/734alpha-lipoic acidoxidative stressventriclesatriacardiac damage |
| spellingShingle | Beata Skibska Anna Goraca Agnieszka Skibska Andrzej Stanczak Effect of Alpha-Lipoic Acid on Rat Ventricles and Atria under LPS-Induced Oxidative Stress Antioxidants alpha-lipoic acid oxidative stress ventricles atria cardiac damage |
| title | Effect of Alpha-Lipoic Acid on Rat Ventricles and Atria under LPS-Induced Oxidative Stress |
| title_full | Effect of Alpha-Lipoic Acid on Rat Ventricles and Atria under LPS-Induced Oxidative Stress |
| title_fullStr | Effect of Alpha-Lipoic Acid on Rat Ventricles and Atria under LPS-Induced Oxidative Stress |
| title_full_unstemmed | Effect of Alpha-Lipoic Acid on Rat Ventricles and Atria under LPS-Induced Oxidative Stress |
| title_short | Effect of Alpha-Lipoic Acid on Rat Ventricles and Atria under LPS-Induced Oxidative Stress |
| title_sort | effect of alpha lipoic acid on rat ventricles and atria under lps induced oxidative stress |
| topic | alpha-lipoic acid oxidative stress ventricles atria cardiac damage |
| url | https://www.mdpi.com/2076-3921/11/4/734 |
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