Biocompatibility and Connectivity of Semiconductor Nanostructures for Cardiac Tissue Engineering Applications
Nano- or microdevices, enabling simultaneous, long-term, multisite, cellular recording and stimulation from many excitable cells, are expected to make a strategic turn in basic and applied cardiology (particularly tissue engineering) and neuroscience. We propose an innovative approach aiming to elic...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/9/11/621 |
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| author | Roberto Gaetani Yuriy Derevyanchuk Andrea Notargiacomo Marialilia Pea Massimiliano Renzi Elisa Messina Fabrizio Palma |
| author_facet | Roberto Gaetani Yuriy Derevyanchuk Andrea Notargiacomo Marialilia Pea Massimiliano Renzi Elisa Messina Fabrizio Palma |
| author_sort | Roberto Gaetani |
| collection | DOAJ |
| description | Nano- or microdevices, enabling simultaneous, long-term, multisite, cellular recording and stimulation from many excitable cells, are expected to make a strategic turn in basic and applied cardiology (particularly tissue engineering) and neuroscience. We propose an innovative approach aiming to elicit bioelectrical information from the cell membrane using an integrated circuit (IC) bearing a coating of nanowires on the chip surface. Nanowires grow directly on the backend of the ICs, thus allowing on-site amplification of bioelectric signals with uniform and controlled morphology and growth of the NWs on templates. To implement this technology, we evaluated the biocompatibility of silicon and zinc oxide nanowires (NWs), used as a seeding substrate for cells in culture, on two different primary cell lines. Human cardiac stromal cells were used to evaluate the effects of ZnO NWs of different lengths on cell behavior, morphology and growth, while BV-2 microglial-like cells and GH4-C1 neuroendocrine-like cell lines were used to evaluate cell membrane–NW interaction and contact when cultured on Si NWs. As the optimization of the contact between integrated microelectronics circuits and cellular membranes represents a long-standing issue, our technological approach may lay the basis for a new era of devices exploiting the microelectronics’ sensitivity and “smartness” to both improve investigation of biological systems and to develop suitable NW-based systems available for tissue engineering and regenerative medicine. |
| first_indexed | 2024-03-09T19:16:30Z |
| format | Article |
| id | doaj.art-c65dc08173c6407b9a8e5de93074ae0a |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-09T19:16:30Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-c65dc08173c6407b9a8e5de93074ae0a2023-11-24T03:46:12ZengMDPI AGBioengineering2306-53542022-10-0191162110.3390/bioengineering9110621Biocompatibility and Connectivity of Semiconductor Nanostructures for Cardiac Tissue Engineering ApplicationsRoberto Gaetani0Yuriy Derevyanchuk1Andrea Notargiacomo2Marialilia Pea3Massimiliano Renzi4Elisa Messina5Fabrizio Palma6Department of Molecular Medicine, “Sapienza” University of Rome, 00176 Rome, ItalyDepartment of Molecular Medicine, “Sapienza” University of Rome, 00176 Rome, ItalyInstitute for Photonics and Nanotechnologies (IFN), National Research Council of Italy, 00133 Rome, ItalyInstitute for Photonics and Nanotechnologies (IFN), National Research Council of Italy, 00133 Rome, ItalyDepartment of Physiology and Pharmacology, “Sapienza” University of Rome, 00176 Rome, ItalyPoliclinico Umberto I, “Sapienza” University of Rome, 00176 Rome, ItalyDepartment Ingegneria dell’ Informazione, Elettronica e Telecomunicazioni, “Sapienza” University of Rome, 00176 Rome, ItalyNano- or microdevices, enabling simultaneous, long-term, multisite, cellular recording and stimulation from many excitable cells, are expected to make a strategic turn in basic and applied cardiology (particularly tissue engineering) and neuroscience. We propose an innovative approach aiming to elicit bioelectrical information from the cell membrane using an integrated circuit (IC) bearing a coating of nanowires on the chip surface. Nanowires grow directly on the backend of the ICs, thus allowing on-site amplification of bioelectric signals with uniform and controlled morphology and growth of the NWs on templates. To implement this technology, we evaluated the biocompatibility of silicon and zinc oxide nanowires (NWs), used as a seeding substrate for cells in culture, on two different primary cell lines. Human cardiac stromal cells were used to evaluate the effects of ZnO NWs of different lengths on cell behavior, morphology and growth, while BV-2 microglial-like cells and GH4-C1 neuroendocrine-like cell lines were used to evaluate cell membrane–NW interaction and contact when cultured on Si NWs. As the optimization of the contact between integrated microelectronics circuits and cellular membranes represents a long-standing issue, our technological approach may lay the basis for a new era of devices exploiting the microelectronics’ sensitivity and “smartness” to both improve investigation of biological systems and to develop suitable NW-based systems available for tissue engineering and regenerative medicine.https://www.mdpi.com/2306-5354/9/11/621biocompatibilityelectronic sensingzinc oxide nanowiressilicon nanowirescardiac tissue |
| spellingShingle | Roberto Gaetani Yuriy Derevyanchuk Andrea Notargiacomo Marialilia Pea Massimiliano Renzi Elisa Messina Fabrizio Palma Biocompatibility and Connectivity of Semiconductor Nanostructures for Cardiac Tissue Engineering Applications Bioengineering biocompatibility electronic sensing zinc oxide nanowires silicon nanowires cardiac tissue |
| title | Biocompatibility and Connectivity of Semiconductor Nanostructures for Cardiac Tissue Engineering Applications |
| title_full | Biocompatibility and Connectivity of Semiconductor Nanostructures for Cardiac Tissue Engineering Applications |
| title_fullStr | Biocompatibility and Connectivity of Semiconductor Nanostructures for Cardiac Tissue Engineering Applications |
| title_full_unstemmed | Biocompatibility and Connectivity of Semiconductor Nanostructures for Cardiac Tissue Engineering Applications |
| title_short | Biocompatibility and Connectivity of Semiconductor Nanostructures for Cardiac Tissue Engineering Applications |
| title_sort | biocompatibility and connectivity of semiconductor nanostructures for cardiac tissue engineering applications |
| topic | biocompatibility electronic sensing zinc oxide nanowires silicon nanowires cardiac tissue |
| url | https://www.mdpi.com/2306-5354/9/11/621 |
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