Immune response and biochemistry of calves immunized with rMSP1a ( Anaplasma marginale) using carbon nanotubes as carrier molecules
Abstract Vaccination against Anaplasma marginale has been considered an important control strategy for bovine anaplasmosis. Recently, mice immunized with rMSP1 a linked to carbon nanotubes (MWNT) showed significant immune responses, generating a new possibility for use of an inactivated vaccine. Th...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Colégio Brasileiro de Parasitologia Veterinaria
2018-05-01
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| Series: | Revista Brasileira de Parasitologia Veterinária |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018005005115&lng=en&tlng=en |
| _version_ | 1818491240347336704 |
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| author | Bruna Torres Silvestre Júlia Angélica Gonçalves da Silveira Elias Jorge Facury-Filho Antônio Último de Carvalho Alice Freitas Versiani Letícia Gracielle Tôrres de Miranda Estevam Márcio Sobreira Silva Araújo Olindo Assis Martins-Filho Deborah Aparecida Negrão-Corrêa Múcio Flávio Barbosa Ribeiro |
| author_facet | Bruna Torres Silvestre Júlia Angélica Gonçalves da Silveira Elias Jorge Facury-Filho Antônio Último de Carvalho Alice Freitas Versiani Letícia Gracielle Tôrres de Miranda Estevam Márcio Sobreira Silva Araújo Olindo Assis Martins-Filho Deborah Aparecida Negrão-Corrêa Múcio Flávio Barbosa Ribeiro |
| author_sort | Bruna Torres Silvestre |
| collection | DOAJ |
| description | Abstract Vaccination against Anaplasma marginale has been considered an important control strategy for bovine anaplasmosis. Recently, mice immunized with rMSP1 a linked to carbon nanotubes (MWNT) showed significant immune responses, generating a new possibility for use of an inactivated vaccine. The objective of this study was to investigate the cellular and humoral responses in calves immunized with MWNT+rMSP1a , associated with inactivated vaccine of A. marginale produced in vitro, and evaluate the toxic effects of the MWNT on renal and hepatic function. rMSP1a was covalently linked to MWNT. Inactivated vaccine (AmUFMG2) was produced by cultivating A. marginale in IDE8 cells. Twenty-four Holstein calves were divided (four groups) and immunized subcutaneously with PBS and non-carboxylated MWNT (control, G1), AmUFMG2 (G2), MWNT+rMSP1a (G3), and AmUFMG2 with MWNT+rMSP1a (G4). Blood samples were collected for total leukocyte counts, biochemical profiling and evaluation of the cellular and humoral response. Immunization with MWNT+rMSP1a induced increase in the total number of leukocytes, NK cells, in the lymphocyte populations and higher levels of antibodies compared to calves immunized only with AmUFMG2. Furthermore, MWNT did not induce changes in the biochemical profile. These data indicate that MWNT+rMSP1a were able to induce the immune responses more efficiently than AmUFMG2 alone, without generating toxicity. |
| first_indexed | 2024-12-10T17:28:09Z |
| format | Article |
| id | doaj.art-c6620ff0896b498ea91ed6cdc029bf09 |
| institution | Directory Open Access Journal |
| issn | 1984-2961 |
| language | English |
| last_indexed | 2024-12-10T17:28:09Z |
| publishDate | 2018-05-01 |
| publisher | Colégio Brasileiro de Parasitologia Veterinaria |
| record_format | Article |
| series | Revista Brasileira de Parasitologia Veterinária |
| spelling | doaj.art-c6620ff0896b498ea91ed6cdc029bf092022-12-22T01:39:46ZengColégio Brasileiro de Parasitologia VeterinariaRevista Brasileira de Parasitologia Veterinária1984-29612018-05-01010.1590/s1984-296120180029S1984-29612018005005115Immune response and biochemistry of calves immunized with rMSP1a ( Anaplasma marginale) using carbon nanotubes as carrier moleculesBruna Torres SilvestreJúlia Angélica Gonçalves da SilveiraElias Jorge Facury-FilhoAntônio Último de CarvalhoAlice Freitas VersianiLetícia Gracielle Tôrres de Miranda EstevamMárcio Sobreira Silva AraújoOlindo Assis Martins-FilhoDeborah Aparecida Negrão-CorrêaMúcio Flávio Barbosa RibeiroAbstract Vaccination against Anaplasma marginale has been considered an important control strategy for bovine anaplasmosis. Recently, mice immunized with rMSP1 a linked to carbon nanotubes (MWNT) showed significant immune responses, generating a new possibility for use of an inactivated vaccine. The objective of this study was to investigate the cellular and humoral responses in calves immunized with MWNT+rMSP1a , associated with inactivated vaccine of A. marginale produced in vitro, and evaluate the toxic effects of the MWNT on renal and hepatic function. rMSP1a was covalently linked to MWNT. Inactivated vaccine (AmUFMG2) was produced by cultivating A. marginale in IDE8 cells. Twenty-four Holstein calves were divided (four groups) and immunized subcutaneously with PBS and non-carboxylated MWNT (control, G1), AmUFMG2 (G2), MWNT+rMSP1a (G3), and AmUFMG2 with MWNT+rMSP1a (G4). Blood samples were collected for total leukocyte counts, biochemical profiling and evaluation of the cellular and humoral response. Immunization with MWNT+rMSP1a induced increase in the total number of leukocytes, NK cells, in the lymphocyte populations and higher levels of antibodies compared to calves immunized only with AmUFMG2. Furthermore, MWNT did not induce changes in the biochemical profile. These data indicate that MWNT+rMSP1a were able to induce the immune responses more efficiently than AmUFMG2 alone, without generating toxicity.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018005005115&lng=en&tlng=enAnaplasma marginaleMSP1avacina inativadananotubos de carbono |
| spellingShingle | Bruna Torres Silvestre Júlia Angélica Gonçalves da Silveira Elias Jorge Facury-Filho Antônio Último de Carvalho Alice Freitas Versiani Letícia Gracielle Tôrres de Miranda Estevam Márcio Sobreira Silva Araújo Olindo Assis Martins-Filho Deborah Aparecida Negrão-Corrêa Múcio Flávio Barbosa Ribeiro Immune response and biochemistry of calves immunized with rMSP1a ( Anaplasma marginale) using carbon nanotubes as carrier molecules Revista Brasileira de Parasitologia Veterinária Anaplasma marginale MSP1a vacina inativada nanotubos de carbono |
| title | Immune response and biochemistry of calves immunized with rMSP1a ( Anaplasma marginale) using carbon nanotubes as carrier molecules |
| title_full | Immune response and biochemistry of calves immunized with rMSP1a ( Anaplasma marginale) using carbon nanotubes as carrier molecules |
| title_fullStr | Immune response and biochemistry of calves immunized with rMSP1a ( Anaplasma marginale) using carbon nanotubes as carrier molecules |
| title_full_unstemmed | Immune response and biochemistry of calves immunized with rMSP1a ( Anaplasma marginale) using carbon nanotubes as carrier molecules |
| title_short | Immune response and biochemistry of calves immunized with rMSP1a ( Anaplasma marginale) using carbon nanotubes as carrier molecules |
| title_sort | immune response and biochemistry of calves immunized with rmsp1a anaplasma marginale using carbon nanotubes as carrier molecules |
| topic | Anaplasma marginale MSP1a vacina inativada nanotubos de carbono |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018005005115&lng=en&tlng=en |
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