Circulating exosomal lncRNA contributes to the pathogenesis of spinal cord injury in rats
Exosome-derived long non-coding RNAs (lncRNAs) are extensively engaged in recovery and repair of the injured spinal cord, through different mechanisms. However, to date no study has systematically evaluated the differentially expressed lncRNAs involved in the development of spinal cord injury. Thus,...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Neural Regeneration Research |
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| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2023;volume=18;issue=4;spage=889;epage=894;aulast=Li |
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| author | Jian-An Li Ming-Peng Shi Lin Cong Ming-Yu Gu Yi-Heng Chen Si-Yi Wang Zhen-Hua Li Chun-Fang Zan Wan-Fu Wei |
| author_facet | Jian-An Li Ming-Peng Shi Lin Cong Ming-Yu Gu Yi-Heng Chen Si-Yi Wang Zhen-Hua Li Chun-Fang Zan Wan-Fu Wei |
| author_sort | Jian-An Li |
| collection | DOAJ |
| description | Exosome-derived long non-coding RNAs (lncRNAs) are extensively engaged in recovery and repair of the injured spinal cord, through different mechanisms. However, to date no study has systematically evaluated the differentially expressed lncRNAs involved in the development of spinal cord injury. Thus, the aim of this study was to identify key circulating exosome-derived lncRNAs in a rat model of spinal cord injury and investigate their potential actions. To this end, we established a rat model of spinal cord hemisection. Circulating exosomes were extracted from blood samples from spinal cord injury and control (sham) rats and further identified through Western blotting and electron microscopy. RNA was isolated from the exosomes and sequenced. The enrichment analysis demonstrated that there were distinctively different lncRNA and mRNA expression patterns between the two groups. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and Gene Ontology (GO) functional analysis were performed to determine the possible involvements of upregulated and downregulated lncRNAs in various pathways and different biological processes, as well as their cellular locations and molecular functions. Furthermore, quantitative reverse transcription-polymerase chain reaction showed that the expression of five lncRNAs––ENSRN0T00000067908, XR_590093, XR_591455, XR_360081, and XR_346933––was increased, whereas the expression of XR_351404, XR_591426, XR_353833, XR_590076, and XR_590719 was decreased. Of note, these 10 lncRNAs were at the center of the lncRNA-miRNA-mRNA coexpression network, which also included 198 mRNAs and 41 miRNAs. Taken together, our findings show that several circulating exosomal lncRNAs are differentially expressed after spinal cord injury, suggesting that they may be involved in spinal cord injury pathology and pathogenesis. These lncRNAs could potentially serve as targets for the clinical diagnosis and treatment of spinal cord injury. |
| first_indexed | 2024-04-13T19:10:37Z |
| format | Article |
| id | doaj.art-c662688c63004aa8804adde45d2239f0 |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-04-13T19:10:37Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-c662688c63004aa8804adde45d2239f02022-12-22T02:33:51ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742023-01-0118488989410.4103/1673-5374.353504Circulating exosomal lncRNA contributes to the pathogenesis of spinal cord injury in ratsJian-An LiMing-Peng ShiLin CongMing-Yu GuYi-Heng ChenSi-Yi WangZhen-Hua LiChun-Fang ZanWan-Fu WeiExosome-derived long non-coding RNAs (lncRNAs) are extensively engaged in recovery and repair of the injured spinal cord, through different mechanisms. However, to date no study has systematically evaluated the differentially expressed lncRNAs involved in the development of spinal cord injury. Thus, the aim of this study was to identify key circulating exosome-derived lncRNAs in a rat model of spinal cord injury and investigate their potential actions. To this end, we established a rat model of spinal cord hemisection. Circulating exosomes were extracted from blood samples from spinal cord injury and control (sham) rats and further identified through Western blotting and electron microscopy. RNA was isolated from the exosomes and sequenced. The enrichment analysis demonstrated that there were distinctively different lncRNA and mRNA expression patterns between the two groups. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and Gene Ontology (GO) functional analysis were performed to determine the possible involvements of upregulated and downregulated lncRNAs in various pathways and different biological processes, as well as their cellular locations and molecular functions. Furthermore, quantitative reverse transcription-polymerase chain reaction showed that the expression of five lncRNAs––ENSRN0T00000067908, XR_590093, XR_591455, XR_360081, and XR_346933––was increased, whereas the expression of XR_351404, XR_591426, XR_353833, XR_590076, and XR_590719 was decreased. Of note, these 10 lncRNAs were at the center of the lncRNA-miRNA-mRNA coexpression network, which also included 198 mRNAs and 41 miRNAs. Taken together, our findings show that several circulating exosomal lncRNAs are differentially expressed after spinal cord injury, suggesting that they may be involved in spinal cord injury pathology and pathogenesis. These lncRNAs could potentially serve as targets for the clinical diagnosis and treatment of spinal cord injury.http://www.nrronline.org/article.asp?issn=1673-5374;year=2023;volume=18;issue=4;spage=889;epage=894;aulast=Liexosome; inflammation; lncrna; microenvironment; mirna; mrna; spinal cord injury; spinal cord repair |
| spellingShingle | Jian-An Li Ming-Peng Shi Lin Cong Ming-Yu Gu Yi-Heng Chen Si-Yi Wang Zhen-Hua Li Chun-Fang Zan Wan-Fu Wei Circulating exosomal lncRNA contributes to the pathogenesis of spinal cord injury in rats Neural Regeneration Research exosome; inflammation; lncrna; microenvironment; mirna; mrna; spinal cord injury; spinal cord repair |
| title | Circulating exosomal lncRNA contributes to the pathogenesis of spinal cord injury in rats |
| title_full | Circulating exosomal lncRNA contributes to the pathogenesis of spinal cord injury in rats |
| title_fullStr | Circulating exosomal lncRNA contributes to the pathogenesis of spinal cord injury in rats |
| title_full_unstemmed | Circulating exosomal lncRNA contributes to the pathogenesis of spinal cord injury in rats |
| title_short | Circulating exosomal lncRNA contributes to the pathogenesis of spinal cord injury in rats |
| title_sort | circulating exosomal lncrna contributes to the pathogenesis of spinal cord injury in rats |
| topic | exosome; inflammation; lncrna; microenvironment; mirna; mrna; spinal cord injury; spinal cord repair |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2023;volume=18;issue=4;spage=889;epage=894;aulast=Li |
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