DNA polymorphism and relationships among the three different riverine populations of spotted snakehead (Channa punctata)

The current study was aimed to investigate the DNA polymorphism of C. punctata from three different rivers of northern region of India. For this, C. punctata of river Ganga and its tributaries (river Gomti and river Ken) was carried out using RAPD-PCR (Random amplified polymorphic DNA-polymerase cha...

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Main Authors: MINAKSHI SINGH, ANKUR KASHYAP, MOHAMMAD SERAJUDDIN
Format: Article
Language:English
Published: Indian Council of Agricultural Research 2021-11-01
Series:Indian Journal of Animal Sciences
Subjects:
Online Access:https://epubs.icar.org.in/index.php/IJAnS/article/view/118160
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author MINAKSHI SINGH
ANKUR KASHYAP
MOHAMMAD SERAJUDDIN
author_facet MINAKSHI SINGH
ANKUR KASHYAP
MOHAMMAD SERAJUDDIN
author_sort MINAKSHI SINGH
collection DOAJ
description The current study was aimed to investigate the DNA polymorphism of C. punctata from three different rivers of northern region of India. For this, C. punctata of river Ganga and its tributaries (river Gomti and river Ken) was carried out using RAPD-PCR (Random amplified polymorphic DNA-polymerase chain reaction). A total of 54 loci were amplified by five selected primers out of 20 having GC content of 60% to 70%. The fish population of river Ganga showed highest genetic polymorphism with 33 (61.11%) polymorphic loci out of 54 loci whereas a total of 55.56% and 46.30% DNA polymorphism were recorded in the fish populations of Ken and Gomti respectively. Maximum genetic identity and minimum genetic distance was recorded between the fish populations of Ganga and Ken showing that these two populations are more similar to each other than to Gomti population. Dendogram based on genetic distance using UPGMA also confirmed that Ganga and Ken population are closer to each other.
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spelling doaj.art-c6639a2eb2234231a695788c71ef49a92023-02-08T11:30:39ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272021-11-01911110.56093/ijans.v91i11.118160DNA polymorphism and relationships among the three different riverine populations of spotted snakehead (Channa punctata)MINAKSHI SINGH0ANKUR KASHYAP1MOHAMMAD SERAJUDDIN2Department of Zoology, Bareilly College, Bareilly, Uttar PradeshJunior High School, Govindpur, Mohanlalganj, Lucknow, Uttar PradeshDepartment of Zoology, University of Lucknow, Lucknow, Uttar PradeshThe current study was aimed to investigate the DNA polymorphism of C. punctata from three different rivers of northern region of India. For this, C. punctata of river Ganga and its tributaries (river Gomti and river Ken) was carried out using RAPD-PCR (Random amplified polymorphic DNA-polymerase chain reaction). A total of 54 loci were amplified by five selected primers out of 20 having GC content of 60% to 70%. The fish population of river Ganga showed highest genetic polymorphism with 33 (61.11%) polymorphic loci out of 54 loci whereas a total of 55.56% and 46.30% DNA polymorphism were recorded in the fish populations of Ken and Gomti respectively. Maximum genetic identity and minimum genetic distance was recorded between the fish populations of Ganga and Ken showing that these two populations are more similar to each other than to Gomti population. Dendogram based on genetic distance using UPGMA also confirmed that Ganga and Ken population are closer to each other.https://epubs.icar.org.in/index.php/IJAnS/article/view/118160Genetic diversityIntraspecific diversityMurrelsRAPDStock
spellingShingle MINAKSHI SINGH
ANKUR KASHYAP
MOHAMMAD SERAJUDDIN
DNA polymorphism and relationships among the three different riverine populations of spotted snakehead (Channa punctata)
Indian Journal of Animal Sciences
Genetic diversity
Intraspecific diversity
Murrels
RAPD
Stock
title DNA polymorphism and relationships among the three different riverine populations of spotted snakehead (Channa punctata)
title_full DNA polymorphism and relationships among the three different riverine populations of spotted snakehead (Channa punctata)
title_fullStr DNA polymorphism and relationships among the three different riverine populations of spotted snakehead (Channa punctata)
title_full_unstemmed DNA polymorphism and relationships among the three different riverine populations of spotted snakehead (Channa punctata)
title_short DNA polymorphism and relationships among the three different riverine populations of spotted snakehead (Channa punctata)
title_sort dna polymorphism and relationships among the three different riverine populations of spotted snakehead channa punctata
topic Genetic diversity
Intraspecific diversity
Murrels
RAPD
Stock
url https://epubs.icar.org.in/index.php/IJAnS/article/view/118160
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