DNA polymorphism and relationships among the three different riverine populations of spotted snakehead (Channa punctata)
The current study was aimed to investigate the DNA polymorphism of C. punctata from three different rivers of northern region of India. For this, C. punctata of river Ganga and its tributaries (river Gomti and river Ken) was carried out using RAPD-PCR (Random amplified polymorphic DNA-polymerase cha...
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Format: | Article |
Language: | English |
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Indian Council of Agricultural Research
2021-11-01
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Series: | Indian Journal of Animal Sciences |
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Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/118160 |
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author | MINAKSHI SINGH ANKUR KASHYAP MOHAMMAD SERAJUDDIN |
author_facet | MINAKSHI SINGH ANKUR KASHYAP MOHAMMAD SERAJUDDIN |
author_sort | MINAKSHI SINGH |
collection | DOAJ |
description | The current study was aimed to investigate the DNA polymorphism of C. punctata from three different rivers of northern region of India. For this, C. punctata of river Ganga and its tributaries (river Gomti and river Ken) was carried out using RAPD-PCR (Random amplified polymorphic DNA-polymerase chain reaction). A total of 54 loci were amplified by five selected primers out of 20 having GC content of 60% to 70%. The fish population of river Ganga showed highest genetic polymorphism with 33 (61.11%) polymorphic loci out of 54 loci whereas a total of 55.56% and 46.30% DNA polymorphism were recorded in the fish populations of Ken and Gomti respectively. Maximum genetic identity and minimum genetic distance was recorded between the fish populations of Ganga and Ken showing that these two populations are more similar to each other than to Gomti population. Dendogram based on genetic distance using UPGMA also confirmed that Ganga and Ken population are closer to each other. |
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format | Article |
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institution | Directory Open Access Journal |
issn | 0367-8318 2394-3327 |
language | English |
last_indexed | 2024-04-10T16:36:03Z |
publishDate | 2021-11-01 |
publisher | Indian Council of Agricultural Research |
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series | Indian Journal of Animal Sciences |
spelling | doaj.art-c6639a2eb2234231a695788c71ef49a92023-02-08T11:30:39ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272021-11-01911110.56093/ijans.v91i11.118160DNA polymorphism and relationships among the three different riverine populations of spotted snakehead (Channa punctata)MINAKSHI SINGH0ANKUR KASHYAP1MOHAMMAD SERAJUDDIN2Department of Zoology, Bareilly College, Bareilly, Uttar PradeshJunior High School, Govindpur, Mohanlalganj, Lucknow, Uttar PradeshDepartment of Zoology, University of Lucknow, Lucknow, Uttar PradeshThe current study was aimed to investigate the DNA polymorphism of C. punctata from three different rivers of northern region of India. For this, C. punctata of river Ganga and its tributaries (river Gomti and river Ken) was carried out using RAPD-PCR (Random amplified polymorphic DNA-polymerase chain reaction). A total of 54 loci were amplified by five selected primers out of 20 having GC content of 60% to 70%. The fish population of river Ganga showed highest genetic polymorphism with 33 (61.11%) polymorphic loci out of 54 loci whereas a total of 55.56% and 46.30% DNA polymorphism were recorded in the fish populations of Ken and Gomti respectively. Maximum genetic identity and minimum genetic distance was recorded between the fish populations of Ganga and Ken showing that these two populations are more similar to each other than to Gomti population. Dendogram based on genetic distance using UPGMA also confirmed that Ganga and Ken population are closer to each other.https://epubs.icar.org.in/index.php/IJAnS/article/view/118160Genetic diversityIntraspecific diversityMurrelsRAPDStock |
spellingShingle | MINAKSHI SINGH ANKUR KASHYAP MOHAMMAD SERAJUDDIN DNA polymorphism and relationships among the three different riverine populations of spotted snakehead (Channa punctata) Indian Journal of Animal Sciences Genetic diversity Intraspecific diversity Murrels RAPD Stock |
title | DNA polymorphism and relationships among the three different riverine populations of spotted snakehead (Channa punctata) |
title_full | DNA polymorphism and relationships among the three different riverine populations of spotted snakehead (Channa punctata) |
title_fullStr | DNA polymorphism and relationships among the three different riverine populations of spotted snakehead (Channa punctata) |
title_full_unstemmed | DNA polymorphism and relationships among the three different riverine populations of spotted snakehead (Channa punctata) |
title_short | DNA polymorphism and relationships among the three different riverine populations of spotted snakehead (Channa punctata) |
title_sort | dna polymorphism and relationships among the three different riverine populations of spotted snakehead channa punctata |
topic | Genetic diversity Intraspecific diversity Murrels RAPD Stock |
url | https://epubs.icar.org.in/index.php/IJAnS/article/view/118160 |
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